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Validated microbiological and HPLC methods for the determination of moxifloxacin in pharmaceutical preparations and human plasma BJM
Abdelaziz,Ahmed A.; Elbanna,Tarek E.; Gamaleldeen,Noha M..
The article presents a comparison between microbiological and high performance liquid chromatographic (HPLC) assays for quantification of moxifloxacin in tablets, ophthalmic solutions and human plasma. The microbiological method employed a cylinder-plate agar diffusion assay using a strain of Esherichia coli ATCC 25922 as the test organism and phosphate buffer (pH8) as the diluent. The calibration curves were linear (R²&gt; 0.98) over a concentration range of 0.125 to 16 µgml-1. The within day and between days precisions were < 4.47% and < 6.39% respectively. Recovery values were between 89.4 and 110.2%. The HPLC assay used Hypersil® BDS C18 reversed phase column (250×4.6 mm, 5µm) with a mobile phase comprising 20 mM ammonium dihydrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Moxifloxacin; Microbiological assay; HPLC assay; Pharmaceutical analysis; Pharmacokinetics.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400008
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Electrochemical biosensors in pharmaceutical analysis BJPS
Gil,Eric de Souza; Melo,Giselle Rodrigues de.
Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosensors; Pharmaceutical analysis; Electrochemical sensors; Enzymes.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300002
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