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Registros recuperados: 18
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Characterization of bacterial endophytes from the roots of native and cultivated Brazil nut trees (Bertholletia excelsa) Acta Amazonica
CHALITA,Patrícia Bombonati; FARIAS,Eliane do Nascimento Cunha; COSTA,Ismaele Breckenfeld da; SOUSA,Brenda Ferreira; SANTOS,Marco Antônio Oliveira dos; ALBUQUERQUE,Teresinha Costa Silveira de; VITAL,Marcos José Salgado; SILVA,Krisle da.
ABSTRACT Brazil nut is a very important nontimber forest product in the Amazon region. Propagation of this tree still represents a challenge due to slow and uneven seed germination. In this context, plant growth-promoting bacteria can facilitate the process of propagation. The aims of this study were to isolate and characterize endophytic bacteria from the roots of Brazil nut trees in native terra firme forest and cultivation areas in northern Brazil, and to identify mechanisms by which bacteria act in plant growth promotion. Overall, 90 bacterial isolates were obtained from the roots of Brazil nut trees in monoculture, agroforestry and native forest areas by using different semisolid media. The isolates were characterized by sequencing the 16S rRNA gene....
Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rRNA; Phosphate solubilization; Indole compounds; Biological nitrogen fixation; Plant growth-promotion bacteria; Amazon.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400257
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Characterization of Pseudomonas bacteria of Piper tuberculatum regarding the production of potentially bio-stimulating compounds for plant growth Acta Amazonica
OLIVEIRA,Danyllo Amaral de; FERREIRA,Solange da Cunha; CARRERA,Daiany Larissa Ribeiro; SERRÃO,Cleyson Pantoja; CALLEGARI,Daihany Moraes; BARROS,Nicolle Louise Ferreira; COELHO,Francinilson Meireles; SOUZA,Cláudia Regina Batista de.
ABSTRACT Despite advances in the identification and characterization of endophytic bacteria in various plant species worldwide, little is known about such microorganisms in plants from the Amazon region. Previous studies reported that Piper tuberculatum endophytic Pseudomonas (isolates Pt12 and Pt13, identified as Pseudomonas putida and Pseudomonas sp., respectively) were able to inhibit the in vitro growth of Fusarium solani f. sp. piperis, which causes root rot in black pepper (Piper nigrum), and that Pt13 promoted the growth of P. nigrum. Therefore, the aim here was to characterize these bacteria regarding their ability to produce plant growth-promoting substances [siderophores, indol acetic acid (IAA) and soluble phosphate]. Chrome azurol S assays were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth-promoting bacterial endophytes; IAA; Siderophores; Phosphate solubilization.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100010
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Enhancement of clover growth by inoculation of P-solubilizing fungi and arbuscular mycorrhizal fungi Anais da ABC (AABC)
Souchie,Edson L.; Azcón,Rosario; Barea,Jose M.; Silva,Eliane M.R.; Saggin-Júnior,Orivaldo J..
This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araxá apatite; Phosphate solubilization; Co-inoculation; Plant growth promotion; Rhizosphere.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300023
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Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil Biota Neotropica
Pontes,Andress P.; Souza,Rocheli de; Granada,Camille E.; Passaglia,Luciane M.P..
The occurrence of associations between bacteria and plant roots may be beneficial, neutral or detrimental. Plant growth promoting (PGP) bacteria form a heterogeneous group of beneficial microorganisms that can be found in the rhizosphere, the root surfaces or in association with host plant. The aim of this study was to isolate and characterize PGP bacteria associated to barley plants (Hordeum vulgare L.) aiming a future application as agricultural inoculant. One hundred and sixty bacterial strains were isolated from roots or rhizospheric soil of barley based on their growth in nitrogen-free selective media. They were evaluated for their ability to produce indolic compounds (ICs) and siderophores, and to solubilize tricalcium phosphate inin vitro assays....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial 16S rRNA gene; Inoculant; Barley; Phosphate solubilization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000200108
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Enhanced solubilization of iron and calcium phosphates by Aspergillus niger by the addition of alcohols BABT
Barroso,Cinthya Babá; Nahas,Ely.
The present study deals with the effect of increased concentrations of alcohols (methanol and ethanol) on the solubilization of FePO4 and CaHPO4 by Aspergillus niger. The efficiency of solubilization (ES) of inorganic phosphates, fungal growth, acid production and pH variation were determined. The concentrations of alcohols that most favored the solubilization of phosphates were 4% methanol and 3% ethanol. The ES of phosphates by the fungus in media containing methanol ranged from 11-96% and 0.4-87% for ethanol. The stimulation or inhibition of solubilization was dependent on greater or lesser fungus growth, acid production and decreased pH. These responses were supported by the correlation between the amount of phosphate dissolved and fungal growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Methanol; Growth; Acid production; Phosphate solubilization.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200003
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Solubilization of CaHPO4 and AlPO4 by Aspergillus niger in culture media with different carbon and nitrogen sources BJM
Barroso,Cinthya Babá; Pereira,Gener Tadeu; Nahas,Ely.
The solubilization of inorganic phosphates by microorganisms supplies phosphates for plant nutrition and increases their growth. The solubilization of CaHPO4 (Ca-P) and AlPO4 (Al-P) by Aspergillus niger using several carbon and nitrogen sources was studied. Solubilization of Ca-P was enhanced when the carbon sources were mannitol, maltose, galactose and glucose (in that order). Galactose, sucrose and maltose were the carbon sources that enhanced the solubilization of Al-P. More extensive growth, acid production, and decrease in pH were obtained in the Al-P medium than in the Ca-P medium, however, the quantity of solubilized phosphate was 12% less. Phosphate solubilization was related to acid production, pH drop and fungal growth in the culture medium. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nutrition; Phosphorus; Phosphate source; Phosphate solubilization; Titratable acidity.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400006
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Characterization of Bacillus isolates of potato rhizosphere from andean soils of Peru and their potential PGPR characteristics BJM
Calvo,Pamela; Ormeño-Orrillo,Ernesto; Martínez-Romero,Esperanza; Zúñiga,Doris.
Bacillus spp. are well known rhizosphere residents of many crops and usually show plant growth promoting (PGP) activities that include biocontrol capacity against some phytopatogenic fungi. Potato crops in the Andean Highlands of Peru face many nutritional and phytophatogenic problems that have a significant impact on production. In this context is important to investigate the natural presence of these microorganisms in the potato rhizosphere and propose a selective screening to find promising PGP strains. In this study, sixty three Bacillus strains isolated from the rhizosphere of native potato varieties growing in the Andean highlands of Peru were screened for in vitro antagonism against Rhizoctonia solani and Fusarium solani. A high prevalence (68%) of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Plant growth-promoting rhizobacteria; Potato; Antagonism; Phosphate solubilization; 16S rRNA gene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400008
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Phosphate solubilization potential and phosphatase activity of rhizospheric Trichoderma spp. BJM
Kapri,Anil; Tewari,Lakshmi.
Trichoderma sp., a well known biological control agent against several phytopathogens, was tested for its phosphate (P) solubilizing potential. Fourteen strains of Trichoderma sp. were isolated from the forest tree rhizospheres of pinus, deodar, bamboo, guava and oak on Trichoderma selective medium. The isolates were tested for their in-vitro P-solubilizing potential using National Botanical Research Institute Phosphate (NBRIP) broth containing tricalcium phosphate (TCP) as the sole P source, and compared with a standard culture of T. harzianum. All the cultures were found to solubilize TCP but with varying potential. The isolate DRT-1 showed maximum amount of soluble phosphate (404.07 µg.ml-1), followed by the standard culture of T. harzianum (386.42...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphate solubilization; Trichoderma; Acid phosphatase; Alkaline phosphatase; Chickpea.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300031
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Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement BJM
Akbar,Shamsa; Sultan,Sikander.
ABSTRACT Background: Since 1960s, the organophosphate pesticide chlorpyrifos has been widely used for the purpose of pest control. However, given its persistence and toxicity towards life forms, the elimination of chlorpyrifos from contaminated sites has become an urgent issue. For this process bioremediation is the method of choice. Results: Two bacterial strains, JCp4 and FCp1, exhibiting chlorpyrifos-degradation potential were isolated from pesticide contaminated agricultural fields. These isolates were able to degrade 84.4% and 78.6% of the initial concentration of chlorpyrifos (100 mg L-1) within a period of only 10 days. Based on 16S rRNA sequence analysis, these strains were identified as Achromobacter xylosoxidans (JCp4) and Ochrobactrum sp....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorpyrifos; Bioremediation; Phosphate solubilization; Achromobacter; IAA.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300563
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Isolation and identification by 16S rRNA sequence analysis of plant growth-promoting azospirilla from the rhizosphere of wheat BJM
Ayyaz,Khadija; Zaheer,Ahmad; Rasul,Ghulam; Mirza,Muhammad Sajjad.
ABSTRACT The main objective of the present study was to isolate phytohormone-producing, phosphate-solubilizing strains of Azospirillum from wheat to be used as inoculants for plant growth promotion. Five Azospirillum strains were isolated from the rhizosphere of field-grown wheat (Triticum aestivum L.), and it was confirmed by BOX-polymerase chain reaction (PCR) that the isolates were different and not re-isolates of the same strain. Sequence analysis of the PCR-amplified 16S rRNA gene indicated that four isolates showed maximum similarity to Azospirillum brasilense and one isolate showed maximum similarity to Azospirillum zeae. This is the first report indicating the presence of an A. zeae like isolate in the wheat rhizosphere in Pakistan. The bacterial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Azospirillum zeae; BOX-PCR; Indole-3-acetic acid (IAA) production; Phosphate solubilization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300542
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Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from rhizosphere of Valeriana jatamansi and its growth promotional effect BJM
Chauhan,Anjali; Guleria,Shiwani; Balgir,Praveen P.; Walia,Abhishek; Mahajan,Rishi; Mehta,Preeti; Shirkot,Chand Karan.
Abstract Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260 mg/L), nitrogen fixation (202.91 nmol ethylene mL-1 h-1), indole-3-acetic acid (IAA) (8.1 µg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1.34%) and oxalic acids. However, genetic evidences for nitrogen fixation and phosphate solubilization by organic acid production have been reported first time...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Valeriana jatamansi; Phosphate solubilization; Nitrogen fixation; Glucose dehydrogenase; Aneurinibacillus aneurinilyticus; PGPR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200294
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Aislamiento y caracterización bioquímica de metabolitos producidos por rizobacterias que solubilizan fosfato. Colegio de Postgraduados
Paredes Mendoza, Marianela.
El empleo de bacterias solubilizadoras de fosfato (BSP) para la solubilización de distintas rocas fosfatadas y de otras fuentes de fósforo es una alternativa para incrementar la cantidad de P disponible para las plantas. Se aislaron 147 BSP de la raíz y rizoplano de diferentes plantas cultivadas. El medio de cultivo denominado Medio Mínimo para Solubilización de Fosfato Colegio de Postgraduados con MgCl (MMSFCP-Mg) fue el más eficiente, debido a que en medio sólido se observaron halos de solubilización de hasta 3.8 cm a los catorce días, y en medio líquido se determinaron hasta 380 mg mL-1 de fosfato soluble a los seis días. Veintisiete de las mejores cepas fueron seleccionadas con base en la eficiencia de solubilización de fosfato...
Palavras-chave: Acidos orgánicos; Fosfatasas; Solubilización de fosfato; Inmunodetección; Roca fosfórica; Bacterias solubilizadoras de fosfato; Enfófitas; Organic acids; Phosphatases; Phosphate solubilization; Immunodetection; Rock phosphate; Phosphate solubilizing; Bacteria; Endophyte; Doctorado; Edafología.
Ano: 2010 URL: http://hdl.handle.net/10521/245
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EFFECTIVENESS OF THE APPLICATION OF ARBUSCULAR MYCORRHIZA FUNGI AND ORGANIC AMENDMENTS TO IMPROVE SOIL QUALITY AND PLANT PERFORMANCE UNDER STRESS CONDITIONS J. Soil Sci. Plant Nutr.
Medina,Almudena; Azcón,Rosario.
Plant growth is limited in arid and/or contaminated sites due to the adverse conditions coming from heavy metal (HM) contamination and/or water stress. Moreover, soils from these areas are generally characterised by poor soil structure, low water-holding capacity, lack of organic matter and nutrient deficiency. In order to carry out a successful re-afforestation, it is necessary to improve soil quality and the ability of plants species to resist this harsh environment. The symbiosis with arbuscular mycorrhizal (AM) fungi has been proposed as one of the mechanisms of plant heavy metal tolerance and water stress avoidance. On the other hand, addition of organic amendments to the soil can reverse degradation of soil properties. Agro-waste residues such as dry...
Tipo: Journal article Palavras-chave: Arbuscular mycorrhiza (AM); Fungi; Organic amendment; Heavy metal (HM) contamination; Water stress; Drought; Organic matter; Rock phosphate; Revegetation; Bioremediation; Aspergillus niger; Phosphate solubilization.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000100009
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Symbiotic effectiveness of Bradyrhizobium ingae in promoting growth of Inga edulis Mart. seedlings Rev. Bras. Ciênc. Solo
Porto,Dilacy Sales; Farias,Eliane do Nascimento Cunha; Chaves,Josimar da Silva; Souza,Brenda Ferreira; Medeiros,Roberto Dantas de; Zilli,Jerri Édson; Silva,Krisle da.
ABSTRACT Inga edulis Mart. is a leguminous tree adapted to acidic and low-fertility soils that establishes symbioses with nitrogen (N)-fixing bacteria. The identification of effective bacteria in biological N fixation may bolster the use of I. edulis in degraded or modified areas and agroforestry systems. Therefore, the aims of this study were evaluation of the symbiotic effectiveness of eight strains of the Bradyrhizobium genus native to Roraima in Inga edulis plants, and in vitro evaluation of the ability of the eight strains of Bradyrhizobium to develop plant growth-promoting characteristics. Determination of symbiotic effectiveness was carried out via three experiments: the first in a greenhouse in pots with a sterile substrate; the second in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological nitrogen fixation; Phosphate solubilization; Indolic compounds.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100403
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Occurrence of plant growth-promoting traits in clover-nodulating rhizobia strains isolated from different soils in Rio Grande do Sul state Rev. Bras. Ciênc. Solo
Vargas,Luciano Kayser; Lisboa,Bruno Brito; Schlindwein,Gilson; Granada,Camille Eichelberger; Giongo,Adriana; Beneduzi,Anelise; Passaglia,Luciane Maria Pereira.
In the last decades, the use of plant growth-promoting rhizobacteria has become an alternative to improve crop production. Rhizobium leguminosarum biovar trifolii is one of the most promising rhizobacteria and is even used with non-legume plants. This study investigated in vitro the occurrence of plant growth-promoting characteristics in several indigenous R. leguminosarum biovar trifolii isolated from soils in the State of Rio Grande do Sul, Brazil. Isolates were obtained at 11 locations and evaluated for indoleacetic acid and siderophore production and inorganic phosphate solubilization. Ten isolates were also molecularly characterized and tested for antagonism against a phytopathogenic fungus and for plant growth promotion of rice seedlings. Of a total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizobacteria; Siderophore; Indoleacetic acid; Phosphate solubilization; Antagonism; Rice.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500016
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ENDOPHYTIC BACTERIA USED AS BIOINOCULANTS IN MICROPROPAGATED BANANA SEEDLINGS Rev. Bras. Frutic.
SOUZA,GLEIKA LARISSE OLIVEIRA DORASIO DE; SILVA,DEIVISSON FERREIRA DA; NIETSCHE,SILVIA; XAVIER,ADELICA APARECIDA; PEREIRA,MARLON CRISTIAN TOLEDO.
ABSTRACT The use of bio-fertilizers and microbial inoculants that promote plant growth and increased yield has been accepted as an alternative to reduce the use of chemical fertilizers. The objective of the present study was to evaluate the effect of plant growth promoting bacteria inoculation on growth and quality of micropropagated banana seedlings ‘Prata Anã’. The experimental design was completely randomized, with four replications and the treatments consisted of 24 isolates of endophytic bacteria. The isolates EB-50 (Bacillus sp.) and EB-133 (Bacillus amyloliquefaciens) characterized as diazotrophic, the EB-51 (Bacillus pumilus) indicate for inorganic phosphate solubilization and EB-55(Bacillus subtilis) and EB-40 (Bacillus sp.) indole-3-acetic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Musa sp; Nitrogen fixation; Phosphate solubilization; IAA; Plant growth promoting bacteria.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000200702
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Isolation and screening for plant growth-promoting (PGP) actinobacteria from Araucaria angustifolia rhizosphere soil Scientia Agricola
Vasconcellos,Rafael Leandro Figueiredo de; Silva,Mylenne Calciolari Pinheiro da; Ribeiro,Carlos Marcelo; Cardoso,Elke Jurandy Bran Nogueira.
Actinobacteria are capable of playing several different roles in soil ecosystems. These microorganisms affect other organisms by producing secondary metabolites and are responsible for the degradation of different complex and relatively recalcitrant organic compounds. In our survey of actinobacteria isolated from the rhizosphere of Araucaria angustifolia, five culture media (AI, WYE, YCED, MSSC and LNMS) were compared for their effectiveness in isolating these microorganisms. When summing up all the isolates randomly obtained, we got 103 isolates. After isolation, the phosphate-solubilizing ability and the "in vitro" production of indole-acetic acid and chitinases were evaluated. The AI medium was ineffective for actinobacteria isolation, when it was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil Pine; Selective culture media; Indole-acetic acid; Chitinase; Phosphate solubilization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600019
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Bacterial strains from floodplain soils perform different plant-growth promoting processes and enhance cowpea growth Scientia Agricola
Costa,Elaine Martins da; Carvalho,Fernanda de; Nóbrega,Rafaela Simão Abrahão; Silva,Jacqueline Savana; Moreira,Fatima Maria de Souza.
ABSTRACT Certain nodulating nitrogen-fixing bacteria in legumes and other nodule endophytes perform different plant-growth promoting processes. The objective of this study was to evaluate 26 bacterial strains isolated from cowpea nodules grown in floodplain soils in the Brazilian savannas, regarding performance of plant-growth promoting processes and ability to enhance cowpea growth. We also identified these strains by 16S rRNA sequencing. The following processes were evaluated: free-living biological nitrogen fixation (BNF), solubilization of calcium, aluminum and iron phosphates and production of indole-3-acetic acid (IAA). The abilities to nodulate and promote cowpea growth were evaluated in Leonard jars. Partial sequencing of the 16S rRNA gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paenibacillus; Biotechnological potential; Biological nitrogen fixation; Phosphate solubilization; Indole-3-acetic acid.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000400301
Registros recuperados: 18
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