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| Hamza, Neila; Silvestre, Frederic; Mhetli, Mohamed; Ben Khemis, Ines; Dieu, Marc; Raes, Martine; Cahu, Chantal; Kestemont, Patrick. |
| A comparative proteomic approach was used to assess the protein expression profile in the liver of 34 days old pikeperch larvae fed from day 10 post hatching, with three isoproteic and isolipidic formulated diets varying by their phospholipid (PL) contents (% dry diet weight): 1.4% (PL1), 4.7% (PL5) and 9.5% (PL9). Using 2D-DIGE minimal labelling of liver extracts, we were able to show 56 protein spots with a differential intensity (p<0.05) depending on the dietary PL content. Among these spots, 11 proteins were un-ambiguously identified using nanoLC-MS/MS tandem mass spectrometry. In the PL9 larvae, our results indicate that the glycolytic pathway could be down-regulated due to the under-expression of the fructose biphosphate aldolase B and the... |
| Tipo: Text |
Palavras-chave: 2D-DIGE; Metabolism; Phospholipid; Pikeperch; Proteomics. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00007/11837/8575.pdf |
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| Hamza, N; Mhetli, M; Ben Khemis, I; Cahu, Chantal; Kestemont, P. |
| This study was carried out to evaluate the effects of dietary phospholipid on the development and rearing performance of pikeperch (Sander lucioperca) larvae. From day 10 post-hatching, fish larvae were weaned onto three isoproteic and isolipidic formulated diets with different phospholipid (PL) levels: 1.4 (PL1), 4.7 (PL5) and 9.5% (PL9) of dry matter, as soybean lecithin. Neutral lipid (NL) with inversed gradient was incorporated in diets. Survival, growth and deformities were monitored until day 34 post-hatching, as well as intestinal enzyme activities, leucine alanine peptidase (leu-ala), aminopeptidase N (AN) and alkaline phosphatase (AP), which were used as indicators of digestive tract maturation. This study showed that PL supplementation... |
| Tipo: Text |
Palavras-chave: Pikeperch; Phospholipids; Larval development; Fatty acid; Digestive enzymes. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4149.pdf |
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