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Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia ArchiMer
Gueguen, Yannick; Montagnani, Caroline; Joubert, Caroline; Marie, Benjamin; Belliard, Corinne; Tayale, Alexandre; Fievet, Julie; Levy, Peva; Piquemal, David; Marin, Frederic; Le Moullac, Gilles; Ky, Chin-long; Garen, Pierre; Lo, Cedrick; Saulnier, Denis.
Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes...
Tipo: Text Palavras-chave: Pearl oyster; Biomineralization; Pinctada margaritifera.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27840/26049.pdf
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Gonad transcriptome analysis of pearl oyster Pinctada margaritifera: identification of potential sex differentiation and sex determining genes ArchiMer
Teaniniuraitemoana, Vaihiti; Huvet, Arnaud; Levy, Peva; Klopp, Christophe; Lhuillier, Emeline; Gaertner-mazouni, Nabila; Gueguen, Yannick; Le Moullac, Gilles.
Background Black pearl farming is based on culture of the blacklip pearl oyster Pinctada margaritifera (Mollusca, lophotrochozoa), a protandrous hermaphrodite species. At first maturation, all individuals are males. The female sex appears progressively from two years old, which represents a limitation for broodstock conditioning for aquaculture production. In marine mollusks displaying hermaphroditic features, data on sexual determinism and differentiation, including the molecular sex determining cascade, are scarce. To increase genomic resources and identify the molecular mechanisms whereby gene expression may act in the sexual dimorphism of P. margaritifera, we performed gonad transcriptome analysis. Results The gonad transcriptome of P. margaritifera...
Tipo: Text Palavras-chave: Pinctada margaritifera; Gametogenesis; Transcriptome; Differential expression; Sex determinism.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00201/31194/29593.pdf
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Problems and perspectives of the pearl oyster aquaculture in French Polynesia. ArchiMer
Cabral, P.
The first attempt to obtain artificial pearls from the black-lip pearl oyster Pinctada margaritifera in French Polynesia dates back to 1963. The pearl oyster culture has grown quickly especially since the eighties. This industry is now the first exportation in value of French Polynesia. 19 atolls of the Tuamotus island have at this time one or more farms and this activity has more and more attractiveness. The rise of this industry has consequently a lot of socio-ecomic repercussions on these atolls. However, as a young industry, the technics and the development are not always controlled and a lot of problems appeared these last years.
Tipo: Text Palavras-chave: French Polynesia; Pinctada margaritifera; Trade; Economic analysis; Harvesting; Hatcheries; Spat; Lagoons; Mortality; Oyster culture; Pearl oysters.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1494.pdf
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Potential combinations of mabé, keshi and cultured pearl production from colourful hatchery-produced Pinctada margaritifera ArchiMer
Ky, Chin-long; Leclerc, Nicolas; Broustal, Floriane; Nakasai, Seiji; Devaux, Dominique.
Aquaculture of nacreous gems, such as cultured pearls, mabé or keshi, is done mostly using different mollusc species grown in countries of the Indo-Pacific region. To date, no single species has been exploited for the simultaneous generation of more than one of these bioproducts, but all require animals with colourful shells. Historically, Pinctada species have mainly been used for nucleated pearl production, selecting the rarer colourful individuals to be used as graft donors. By contrast, colourful Pteria species have mostly been used for mabé production, as the grafting operation for pearl production is associated with low yield. In this study, we report the potential for cumulating cultured pearl and mabé (MP), or keshi and mabé (MK) production, using...
Tipo: Text Palavras-chave: Pearl oyster; Pinctada margaritifera; Mabe; Keshi; Pearls.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00482/59398/62168.pdf
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Evidence of donor effect on cultured pearl quality from a duplicated grafting experiment on Pinctada margaritifera using wild donors ArchiMer
Tayale, Alexandre; Gueguen, Yannick; Treguier, Cathy; Le Grand, Jacqueline; Cochennec-laureau, Nathalie; Montagnani, Caroline; Ky, Chin-long.
Producing high quality cultured black pearls from Pinctada margaritifera is one of the major challenges for the "pearl oyster" industry in French Polynesia. In order to assess donor effect on cultured pearl quality, wild Pinctada margaritifera originating from the Tuamotu Archipelago were used in a duplicated grafting experiment. After 12 months of culture, nucleus retention was assessed and seven pearl quality traits recorded on the 454 cultured pearls harvested from the experiment. The traits scored were nacre thickness and pearl weight, surface defects, lustre, grade, and the colour components: 1) darkness of cultured pearl colour, and 2) visual perception of colour class (bodycolor and/or overtone). Our results demonstrate for the first time that...
Tipo: Text Palavras-chave: Cultured pearl oyster; Pearl quality; Nacre; Donor mollusc; Genetic selection; Pteriidae; Blacklip pearl oyster; Pinctada margaritifera; French Polynesia.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00116/22757/20541.pdf
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Tracing key genes associated with the Pinctada margaritifera albino phenotype from juvenile to cultured pearl harvest stages using multiple whole transcriptome sequencing ArchiMer
Auffret, Pauline; Le Luyer, Jeremy; Sham Koua, Manaarii; Quillien, Virgile; Ky, Chin-long.
Background Albino mutations are commonly observed in the animal kingdom, including in bivalves. In the black-lipped pearl oyster Pinctada margaritifera, albino specimens are characterized by total or partial absence of colouration resulting in typical white shell phenotype expression. The relationship of shell colour with resulting cultured pearl colour is of great economic interest in P. margaritifera, on which a pearl industry is based. Hence, the albino phenotype provides a useful way to examine the molecular mechanisms underlying pigmentation. Results Whole transcriptome RNA-sequencing analysis comparing albino and black wild-type phenotypes at three stages over the culture cycle of P. margaritifera revealed a total of 1606, 798 and 187 differentially...
Tipo: Text Palavras-chave: Pinctada margaritifera; Albinism; Pigmentation; RNA-seq; Notch signaling pathway; Tyrosinase; Biomineralization.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00652/76420/77477.pdf
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Development of cultured pearl circles and shape after initial graft and second nucleus insertion in the black-lipped pearl oyster Pinctada margaritifera ArchiMer
Ky, Chin-long; Demmer, Jonathan; Sham-koua, Manaarii; Cabral, Philippe.
Production of larger, rounder, high-quality cultured pearls with fewer circles is one of the main challenges of Pinctada margaritifera aquaculture faced by every pearl farm in French Polynesia. Although bigger pearl sizes can be achieved through surgreffe operations (implantation of a second nucleus following pearl harvest), control of the development of pearl circles and shapes still remains unclear, as illustrated by grafter’s empirical rules, where often the surgreffe process is only performed after production of uncircled and round pearl shapes. The present study was designed with a real pearl by pearl traceability to reveal for the first time the development of circles and shapes from graft and surgreffe, in relation to the size of the pearl sac. This...
Tipo: Text Palavras-chave: Pearl oyster; Pinctada margaritifera; Surgreffe; Circled pearls; Pearl shapes; Pearl sac.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00276/38690/37187.pdf
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Impact of spat shell colour selection in hatchery-produced Pinctada margaritifera on cultured pearl colour ArchiMer
Ky, Chin-long; Sham Koua, Manaarii; Le Moullac, Gilles.
Beaded cultured pearl farming is a lengthy aquaculture process, particularly when the pearl oysters are produced through a hatchery propagation system, and includes the key steps of artificial breeding, larval and spat rearing before graft operations can take place. Within its genus, Pinctada margaritifera has the ability to produce the widest range of pearl colours, thanks to the donor colour polymorphism of the inner shell, which is mainly responsible for colour transmission. As hatchery spat production in P. margaritifera leads to several colour phenotypes (at 3 months old), the aim of this study was to determine whether a relation exists between the colour of the donors as spat and the final pearl colour. In the experiment, which took place over a...
Tipo: Text Palavras-chave: Pinctada margaritifera; Phenotypes; Spat shell colour; Earlier selection; Pearl colour.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00418/52969/53895.pdf
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Development of an antiseptic procedure to improve retention rates during the grafting process of black-lip oyster, Pinctada margaritifera ArchiMer
Cochennec, Nathalie; Haffner, Philippe; Levy, Peva; Saulnier, Denis; Fougerouse, A.; Langy, Sandra.
In French Polynesia, black pearl cultivation now takes place on about 30 of the 118 atolls or islands. This activity has largely replaced copra, coconut oil and fishing as a source of income. Around 1,000 pearl farms, generally small ones of less than 5 ha, are registered. The pearl farms are mainly owned by families. In 1998, about 7,000 persons were employed in theentire pearl industry, from production to retailing. In value, this activity represents the first exportation of French Polynesian products. From 1988 to date, exports increased exponentially before leveling. Starting with less than two kilograms in 1972, exports have now stabilized at Il tons of "black" pearls.
Tipo: Text Palavras-chave: Vibrio; Microbiology; Grafting process; Pinctada margaritifera.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-4928.PDF
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Etude et modélisation des mécanismes impliqués dans la croissance de l'huître perlière, Pinctada margaritifera, au sein de l'écosystème conchylicole du lagon de l'atoll de Takapoto (Polynésie Française) ArchiMer
Pouvreau, Stephane.
L'huître perlière, Pinctada margaritifera (Linné, 1758) var. cumingi (Reeve), productrice de perles noires, fait l' objet d'une aquaculture florissante en Polynésie Française. Afin de pérenniser cette activité, un programme de recherche (le PGRN) a été lancé, en 1990, sur l' atoll de Takapoto. L' un des thèmes de ce programme réside dans l'étude de la capacité trophique d'un lagon soumis à une activité de perliculture. A cet égard, un modèle déterministe expliquant la croissance et la reproduction de P. margaritifera à partir des paramètres du milieu (notamment la nourriture disponible) a été mis au point. La construction de ce modèle a impliqué : ( 1) l'analyse de l'hydrobiologie du lagon; (2) l'étude de la physiologie de P. margaritifera; (3) le sui vi...
Tipo: Text Palavras-chave: Bivalves; Huîtres perlières; Pinctada margaritifera; Physiologie; Croissance; Reproduction; Hydrobiologie; Atoll; Lagon; Écosystème; Réseau trophique; Modélisation; Capacité trophique; Polynésie française; Bivalves; Pearl oyster; Pinctada ma rgaritifera; Physiology; Growth; Reproduction; Hydrobiology; Atoll; Lagoon; Ecosystem; Trophic web; Modelling; Carrying capacity; French Polynesia.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00034/14558/11856.pdf
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Some aspects of the abnormal mortalities of the pearl oysters, Pinctada margaritifera in the Tuamotu Archipelago, (French Polynesia) ArchiMer
Cabral, P.
The black-lip pearl oyster Pinctada margaritifera lives and reproduces with high performance in most of Tuamotu Lagoons. Technics to collect spats, to culture juveniles and to operate oysters were quickly found and applicated. Nevertheless, the sudden rise of this activity has been correlated with an anarchic development. For instance, large occupation of areas in the lagoons, cultivation of numerous oysters in confined areas, lack of knowledge on the basis culture technics in many farms. In 1985, mass mortalities have appeared in some lagoons and 50 to 80% of cultured stock died. Some investigations have been done but no explanation was found. In 1988, the mortalities are still very high. An analysis of cooperative production results from 1979 to 1988...
Tipo: Text Palavras-chave: Pinctada margaritifera; Bivalvia; Spat; Juveniles; Oyster culture; Reproduction; Pearl culture; Pearl oysters; Mortality.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1484.pdf
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Abnormal periostracum secretion during the mineralization process of the pearl in the blacklip pearl oyster Pinctada margaritifera ArchiMer
Comps, Marie-annick; Herbaut, Christian; Fougerouse, Angélique.
Whitish parasite-like bodies (PLBs), 0.5-2 mu m in size, were detected within the pearl bag of some grafted pearl oysters, Pinctada margaritifera, from Takapoto island (French Polynesia). Histological and ultrastructural investigations have revealed that these bodies consisted of lamellar organic material locally secreted by some epithelial cells of the pearl bag. The fine structure and the formation of these bodies are described. The causes of this abnormal secretion of organic mater, which actually corresponds to periostracum, are not presently known. No parasite nor infectious micro-organism was found associated with the PLBs.
Tipo: Text Palavras-chave: Periostracum; Pearl bag; Pinctada margaritifera.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-545.pdf
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Virus-like particles in pearl oyster Pinctada margaritifera ArchiMer
Comps, Michel; Herbaut, Ch.; Fougerouse, A..
Virus-like particles were detected in granulomas associated with focal necrosis within the adductor muscle of the pearl oyster Pinctada margaritifera from French Polynesia. About 40 nm in size, the se particles were generally found within cytoplasmic vesic1es inside heavily degenerated granulocytes. More studies are required to further characterize them.
Tipo: Text Palavras-chave: Pinctada margaritifera; Pathology; Pearl oyster; Virus like.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-6079.PDF
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Growth Performance Comparison of Pinctada margaritifera Juveniles Produced by Thermal Shock or Gonad Scarification Spawning Procedures ArchiMer
Ky, Chin-long; Lau, Christophe; Sham Koua, Manaarii; Lo, Cedrik.
The black-lipped pearl oyster Pinctada margaritifera is of high economic importance in French Polynesia. Arecent decline in pearl value led to the initiation of several genetic breeding programs aiming to improve production traits, including oyster shell growth, which dictates the time of grafting, size of the implanted nuclei, and biomineralization capacities. We assessed shell diameters on hatchery-produced spat to analyze juvenile growth performance of four half-sib families derived from polyandry (one dam crossed with two sires) and polygyny (two dams crossed with one sire) using gametes obtained by thermal shock or gonad scarification. Spat growth was monitored over 3 mo, with shell diameter measured weekly. Results revealed that the spawning method...
Tipo: Text Palavras-chave: Pearl oysters; Pinctada margaritifera; Juvenile; Growth performance; Spawning procedure.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00310/42167/41470.pdf
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Gestion des ressources génétiques de l'huître perlière Pinctada margaritifera de Polynésie française : caractérisation génétique des populations et optimisation du recrutement pour l'exploitation perlière ArchiMer
Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre.
Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different...
Tipo: Text Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF
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Colour or no colour in the juvenile shell of the black lip pearl oyster, Pinctada margaritifera? ArchiMer
Trinkler, Nolwenn; Le Moullac, Gilles; Cuif, Jean-pierre; Cochennec-laureau, Nathalie; Dauphin, Yannicke.
Pinctada margaritifera mollusc is cultivated in French Polynesia for the production of black pearls. For this study, the colour of juvenile samples (48 days old) was investigated in the visible range spectra (430-700 nm) using spectrophotometry. A first measurement was done with the soft parts still inside the shell (entire animal). Then, the soft parts were removed in order to do a second measurement on the growing edge of the shell. Comparison of the two measurements shows that the estimation of the living animal colour with unaided eye is strongly influenced by the colour of the soft parts. The use of the International Commission on Illumination (ISI) chromaticity diagram shows that at this growth stage, the shells are "white"; i.e. present no...
Tipo: Text Palavras-chave: Juvenile shell; Colour-scoring; Mollusc; Pearl oyster; Pinctada margaritifera.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18883/16449.pdf
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Estimation of physical and physiological performances of blacklip pearl oyster larvae in view of DEB modeling and recruitment assessment ArchiMer
Sangare, Nathanael; Lo-yat, Alain; Le Moullac, Gilles; Pecquerie, Laure; Thomas, Yoann; Beliaeff, Benoit; Andrefouet, Serge.
In French Polynesia black pearl farming represents one of the dominant business sectors. However, it still entirely relies on unpredictable Pinctada margaritifera spat collection success, which is itself conditioned by larval development completion. To assess the relationship between larval development and recruitment success, we studied under controlled conditions the effect of food concentration on development, growth, ingestion rate, survival and metabolic rate at the larval stage. Larvae were exposed to four different phytoplankton densities (2,5; 7,5; 15 and 30 cell.μL−1). Larvae survived equally all over the range of phytoplankton concentration with an average survival rate of 16% at the end of experiments. Food concentration significantly affected...
Tipo: Text Palavras-chave: Bivalve larvae; Pinctada margaritifera; Physiology; Energetics; Dynamic energy budget theory; Pearl farming; Aquaculture.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00476/58795/61446.pdf
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Influence of preoperative food and temperature conditions on pearl biogenesis in Pinctada margaritifera ArchiMer
Latchere, Oihana; Le Moullac, Gilles; Gaertner-mazouni, Nabila; Fievet, Julie; Magre, Kevin; Saulnier, Denis.
Trophic conditions and water temperature strongly influence bivalve physiological processes and metabolism. In black-lip pearl oyster Pinctada margaritifera, these parameters have been shown to affect shell biomineralization. The present study investigated the effect of preoperative food level (i.e., microalgal concentration) and temperature on pearl biomineralization. Donor and recipient oysters were conditioned at different levels of food and temperature during the preoperative phase to evaluate the influence of these factors on 1) pearl retention rate (grafting success), 2) expression of genes involved in biomineralization in the mantle and pearl sac and 3) pearl quality traits. Our study confirmed the influence of both microalgal concentration and...
Tipo: Text Palavras-chave: Pinctada margaritifera; Cultured pearl; Biomineralization; Environment; Gene expression; Pearl quality.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00387/49799/50367.pdf
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Etude cytologique de la gamétogenèse, de la sex-ratio et du cycle de reproduction chez l'huître perlière Pinctada margaritifera (L) var. cumingii (Jameson), (mollusques, bivalves). Comparaison avec le cycle de Pinctada maculata (Gould) ArchiMer
Thielley, Maryse.
The population of Pinctada margaritifera and Pinctada maculata lay eggs throughout the year. Temperature variations induce huge but asynchronous laying. Preferential periods of gonad maturity correspond to the season changings in both species. However. some differences between the two specics are noticed. Both species are protandric hermaphrodites. Female condition is unstable: a reversion to the male condition may be observed and is probably linked with stress. The average size for the first sexual maturation as weil as for sexual conversion are specified in P. margaritifera. The breeding conditions induce modifications in some reproductive cycle processes (maturation, sex-ratio). Some conditions suitable for reproduction may become unfavorable to...
Tipo: Text Palavras-chave: Gamétogenèse; Cycle de reproduction; Sex-ratio; Pinctad margaritifera; Mollusque; Bivalve; Huître perlière; Gametogenesis; Reproductive cycle; Sex-ratio; Pinctada margaritifera; Mollusca; Bivalvia; Pearl oyster.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00440/55208/56677.pdf
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The pearl oyster (Pinctada margaritifera) aquaculture in French Polynesia and the indirect impact of long-distance transfers and collection-culture site combinations on pearl quality traits ArchiMer
Ky, Chin-long; Broustal, Floriane; Potin, Dan; Lo, Cedrik.
In French Polynesia, the P. margaritifera pearl aquaculture industry is spread over a vast area, as large as Europe. All the oysters for this the highly economically important activity are supplied from just a few collection lagoons, but they are grown in numerous sites across three archipelagos (Gambier, Society and Tuamotu). Many oyster transfers thus indirectly bring about grafting combinations mixing different geographic origins and production sites. This study aims to examine the impact of such graft combinations on cultured pearl quality traits. For this, six homogeneous and standardised experimental graft combinations (N = 6197) were conducted at commercial scale in the two growing locations the most frequently used in French Polynesia: Arutua atoll...
Tipo: Text Palavras-chave: Pearl oyster; Pinctada margaritifera; Graft combinations; Pearl quality traits; Environment.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00477/58888/61444.pdf
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