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The complete chloroplast genome sequences of three Spondias species reveal close relationship among the species Genet. Mol. Biol.
Santos,Vanessa; Almeida,Cícero.
Abstract This study reports the complete chloroplast sequences of three Spondias species. The genome sequences were obtained for Spondias tuberosa, Spondias bahienses, and Spondias mombin using the Illumina sequencing technology by a combination of de novo methods and a reference-guided assembly using Sapindus mukorossi as reference. The genomes of S. tuberosa, S. bahiensis, and S. mombin had 162,036, 162,218, and 162,302 bp, respectively. The coding regions exhibited 130 genes, including 34–35 tRNAs and 4 rRNAs. The results revealed synteny among the genomes, with high conservation in the gene order and content and CG content. The inverted repeat regions (IRA and IRB) and the large and small single copies were very similar among the three genomes. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plastome; Evolution; Spondias.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100132
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Cloning and sequence analysis of tomato cpDNA fragments: towards developing homologous chloroplast transformation vectors Braz. J. Plant Physiol.
Berger,Irving Joseph; Carraro,Dirce Maria; Bock,Ralph; Azevedo,Ricardo Antunes; Carrer,Helaine.
With the view of developing chloroplast transformation vectors based on homologous targeting regions for tomato (Lycopersicon esculentum L.), plastid DNA fragments of tomato cv. IAC-Santa Clara were cloned and analyzed. Isolation and cloning of PstI/SalI chloroplast fragments into the pBlueScript vectors yielded the plasmids pIJB1 and pIJB2 with cpDNA fragments of 8.6 kb and 6.4 kb, respectively. DNA sequencing and computer analyses revealed that the tomato sequences cloned display from 93 to 100 % of identity to the respective fragments in tobacco, which is more pronounced in coding regions. The intergenic spacers are somewhat less conserved suggesting that evolutionary divergences occurred mainly in these putative non-coding regions. The most remarkable...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Nicotiana tabacum; Chloroplast DNA; Plastid transformation; Plastome; Tobacco; Tomato.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200007
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