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	Provedor de dados BABT (3) Autor Bacci Jr.,Maurício (1) Furlan,Antonio (1) Gunasekaran,P. (1) Hajjari,Mohammadreza (1) Kannan,T.R. (1) Martins,Vanderlei Geraldo (1) Miranda,Vitor Fernandes Oliveira de (1) Raj,K. Chandra (1) Saffar,Behnaz (1) Sangiliyandi,G. (1) Mais... Palavra-chave Polymerase (3) Amplification primer (1) DNA (1) Drosera (1) Efficiency (1) Fungi (1) In vitro mutagenesis (1) Levan (1) Levansucrase (1) Phylogeny (1) Selective modulation (1) Single step assembly PCR (1) Sucrase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2011 (1) 2010 (1) 1999 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification BABT Miranda,Vitor Fernandes Oliveira de; Martins,Vanderlei Geraldo; Furlan,Antonio; Bacci Jr.,Maurício. The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers. The analysis of the products demonstrated most samples were a molecular mixture as a result of unsuccessful and non-specific amplifications. Among the obtained sequences, two were from Basidiomycota fungi. Homologous sequences of Basidiomycota were obtained from GenBank database and added to a data set with sequences from Drosera leaves. Parsimony analysis demonstrated that one sequence was amplified from an Ustilaginomycetes fungus, and another from a Heterobasidiomycetes. Possibly these fungi were associated to leaves of Drosera, and not because of samples contamination. In order to provide optimization and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase; DNA; Drosera; Fungi; Phylogeny. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100018 Separation of levan-formation and sucrose-hydrolysis catalyzed by levansucrase of Zymomonas mobilis using in vitro mutagenesis BABT Sangiliyandi,G.; Kannan,T.R.; Raj,K. Chandra; Gunasekaran,P.. A levansucrase (SacB) of Zymomonas mobilis capable of sucrose hydrolysis but not levan formation was isolated through invitro mutagenesis of cloned sacB gene. When the sacB mutant gene was expressed in Escherichiacoli strains, only 50% of the sucrose-hydrolysing activity (2.0 U/mg) was produced, compared to the wild type levansucrase (4.0 U/mg). Sequencing of the sacB mutant gene revealed changes of two amino acid residues (Phe-102 to Leu and Trp-261 to Lys in the levansucrase). The absence of mutation at the site of Cys of SacB is contradictory to the inhibition kinetics that demonstrated the involvement of Cys in conferring the levan-forming activity to the SacB. The present finding is useful in understanding the mechanism of selective modulation of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Levansucrase; Polymerase; Sucrase; In vitro mutagenesis; Levan; Selective modulation. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400001 The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis BABT Hajjari,Mohammadreza; Saffar,Behnaz. We have constructed a short gene with a single step assembly PCR without any additional amplification primers with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions, we have analyzed various effects on its efficiency. Eventually, we have been able to synthesize the gene in less than 40 minutes by Taq polymerase. Tipo: Info:eu-repo/semantics/article Palavras-chave: Single step assembly PCR; Efficiency; Amplification primer; Polymerase. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100007 Registros recuperados: 3 Primeira ... 1 ... Última

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