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Registros recuperados: 29 | |
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WISBECK,ELISABETH; FACCHINI,JEAN M.; ALVES,ENDI P.; SILVEIRA,MARCIA L.L.; GERN,REGINA M.M.; NINOW,JORGE L.; FURLAN,SANDRA A.. |
ABSTRACT Fungi of Pleurotus genus have attracted a great interest due to their medicinal properties such as anti-inflammatory, antimicrobial and antitumor. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. This work aimed to study the mycelial growth of P. ostreatus in submerged culture, evaluating the influence of the initial concentration of substrate (20 and 40 g/L of glucose) and the pH (4 and 6) on kinetic parameters of production of biomass. The effectiveness of different doses (10, 30 and 50 mg/kg) of a mycelium polysaccharide fraction extracted from P. ostreatus in reducing Sarcoma 180 development in mice was also verified. In the range of this study, maximum concentration of mycelial biomass (about 12.8 g/L) was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antitumor Activity; Mycelium; Pleurotus ostreatus; Polysaccharides; Sarcoma 180. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502013 |
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Verçosa Júnior,D.; Ferraz,V.P.; Duarte,E.R.; Oliveira,N.J. F.; Soto-Blanco,B.; Cassali,G.D.; Melo,M.M.. |
The aim of this study was to investigate the effect of the mushroom Agaricus blazeii Murril (ABM) extracts on the hematological profile of Swiss mice bearing an Ehrlich solid tumor. Three fractions (total extract, polysaccharides, and supernatant) of ABM extracts obtained by four methods (ultrasonic or water bath, at pH 4 or pH 7) were administered to mice over 21 days. Polysaccharide solutions were analyzed by gas and liquid chromatography that showed both mannose and glucose concentrations. The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. The treatment with ABM supernatant at pH 7 and water bath was associated with reduced concentrations of leukocytes and lymphocytes and altered the percentage... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mouse; Ehrlich solid tumor; Polysaccharides; Hematological profile. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300679 |
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MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
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Breder,J.S.C.; Pires,A.L.R.; Azevedo,F.F.; Apolinário,P.P.; Cantaruti,T.; Jiwani,S.I.; Moraes,Â.M.; Consonni,S.R.; Araújo,E.P.; Adams,G.G.; Saad,M.J.A.; Lima,M.H.M.. |
The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C57BL/6J mice; Wound healing; Wound dressing; Polysaccharides; Diabetes. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100602 |
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Gil Cortiella,Mariona; Peña-Neira,Álvaro. |
Obtaining polysaccharides from grape cell walls is an interesting topic for the wine industry given its implications with regards to wine treatments and potential sensory attributes. In the present work, several conditions (i.e., a chelating agent, pH, titratable acidity, extraction time, number of successive extractions, solid to liquid ratio and alcoholic fermentation) were tested to assess their effects on the yield of soluble polysaccharides obtained from grape skins. Among the four tested solvents (i.e., water, tartaric acid, ammonium oxalate, and ethylenediaminetetraacetic acid), aqueous ammonium oxalate was the most effective for polysaccharide extraction but also produced the highest amount of protein impurities. Increasing the number of successive... |
Tipo: Journal article |
Palavras-chave: Carménère; Extraction; Grape skin polysaccharides; Pectin; Polysaccharides; Wine. |
Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000100009 |
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BUSTILLOS-RODRÍGUEZ,Juan Carlos; TIRADO-GALLEGOS,Juan Manuel; ORDÓÑEZ-GARCÍA,Magali; ZAMUDIO-FLORES,Paul Baruk; ORNELAS-PAZ,José de Jesús; ACOSTA-MUÑIZ,Carlos Horacio; GALLEGOS-MORALES,Gabriel; PÁRAMO-CALDERÓN,Delia Esther; RIOS-VELASCO,Claudio. |
Abstract Native starch was isolated from two corn landraces (blue and white) and a hybrid variety and then characterized for its physicochemical, thermal and rheological properties. The hybrid variety showed the highest starch yield, with its starch showing the highest fat, moisture and protein content but the lowest onset temperature of gelatinization. The hybrid corn starch also showed the highest G’ and G” values. The tested starches showed similar Tp, Tc, and ΔH values. The amylose contents ranged from 21.5 to 22.4%, with starch from blue corn showing the highest amylose content. All starches were white, with L values ranging from 93.6 to 99.5. The light absorbance of the starch gels increased with storage time. The obtained results demonstrated that... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corn type; Maize; Polysaccharides; Functionality; Technological uses; Bromatological composition. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000100149 |
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YAN,Wensheng; JIANG,Lingjun; XU,Jifen. |
Abstract This study aimed to investigate the effects of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharides (CPP) on the glucose-lipid metabolism in T2DM rats. T2DM model was established in 40 Wistar rats which were then randomly divided into model, CPP-1, CPP-2 and CPP-3 groups. Ten normal rats were selected as the control group. The CPP-1, CPP-2 and CPP-3 group were intragastrically administrated with 100, 200 and 400 mg/kg CPP for 4 weeks, respectively. Results showed that, during the treatment, compared with model group, in CPP treatment groups the T2DM symptoms were lightened, and the body weight was improved. After treatment, compared with model group, in CPP-3 group the blood fasting plasma glucose, fasting insulin and glycosylated hemoglobin... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyclocarya paliurus (Batal.) Iljinskaja; Polysaccharides; Type 2 diabetes mellitus; Inflammatory response; Oxidative stress. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005028102 |
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García Ruiz, Mayra Teresa. |
Se evaluaron los cambios de acidez titulable a lo largo de 24 h, de las yemas vegetativas con longitud media de 0.5 cm y nopalitos de 6 y 11 cm, de Opuntia ficus-indica de los cultivares Atlixco, Copena V1, Milpa Alta, Moradaza y Solferino, con riego. También se evaluó el efecto de la disminución del potencial de agua (ΨA) del suelo, por suspensión del riego durante 60 días, en condiciones de invernadero, en el potencial osmótico (ΨS ), liberación de electrolitos (o índice de daño membranal, Id) y contenido de polisacáridos estructurales de los nopalitos de esos cinco cultivares y una recolecta silvestre de O. streptacantha. La acidez de las yemas vegetativas y los nopalitos fluctuó entre 0.2 y 0.9 % a lo largo de las 24 horas del día, con las... |
Tipo: Tesis |
Palavras-chave: Acidez titulable; Potencial hídrico; Potencial osmótico; Polisacáridos; Maestría; Fisiología Vegetal; Tritable acidity; Water potential; Osmotic potential; Polysaccharides. |
Ano: 2007 |
URL: http://hdl.handle.net/10521/1654 |
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Chang,Ermei; Zhao,Yuxin; Wei,Qianchun; Shi,Shengqing; Jiang,Zeping. |
Platycladus orientalis has a lifespan of several thousand years in China, making it a good plant in which to study aging at the molecular level, but this requires sufficient quantities of high-quality P. orientalis RNA. However, no appropriate methods have been reported for total RNA isolation from P. orientalis leaves. The TRIzol method did not extract RNA, while cetyltrimethylammonium bromide, sodium dodecyl sulfate-phenol, and plant RNAout kit (Tianz, Inc., China) protocols resulted in low yields of poor quality RNA. Isolating total RNA using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) resulted in a high-quality product but a low yield. However, the two-step removal of polyphenols and polysaccharides in the improved plant... |
Tipo: Journal article |
Palavras-chave: Cupressaceae plants; Polysaccharides; Polyphenols; Total RNA isolation; Two-step removal of polyphenols and polysaccharides. |
Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500004 |
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Rubio-Piña,Jorge A; Zapata-Pérez,Omar. |
RNA extraction from mangrove tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds upon cell disruption. Besides, a successful RNA isolation from mangrove tissues sometimes can be strain and species specific. Two different methods were used to extract RNA from tissues (stems, leaves and roots) of black mangrove (Avicennia germinans) and white mangrove (Laguncularia racemosa), collected from the mangrove area at Progreso, Yucatan, Mexico. An optimized and modified total RNA isolation method was developed for these recalcitrant species. The protocol is based on the CTAB method including β-mercaptoethanol and PVPP with sequential Phenol/Chloroform/isoamyl alcohol extractions to remove protein, and polyphenols,... |
Tipo: Journal article |
Palavras-chave: Mangrove; Plant tissues; Polysaccharides; RNA isolation. |
Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500011 |
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Senni, Karim; Gueniche, Farida; Changotade, Sylvie; Septier, Dominique; Sinquin, Corinne; Ratiskol, Jacqueline; Lutomski, Didier; Godeau, Gaston; Guezennec, Jean; Colliec-jouault, Sylvia. |
Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800... |
Tipo: Text |
Palavras-chave: Marine hydrothermal bacteria; Glycosaminoglycan-mimetic; Collagen; Matrix metalloproteinases; Dermal fibroblast; Polysaccharides; Tissue engineering. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00138/24973/23072.pdf |
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Varenne, A; Gareil, P; Colliec-jouault, Sylvia; Daniel, R. |
The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to... |
Tipo: Text |
Palavras-chave: Antithrombin; Fucoidan; Electrophoresis; Protein interaction; Polysaccharides. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2114.pdf |
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Registros recuperados: 29 | |
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