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| Lima-Verde,I.B.; Matos,M.H.T.; Bruno,J.B.; Martins,F.S.; Santos,R.R.; Báo,S.N.; Luque,M.C.A.; Vieira,G.A.B.; Silveira,E.R.; Rodrigues,A.P.R.; Figueiredo,J.R.; Oliveira,M.A.L.; Lima,P.F.. |
| The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Caprine; Preantral follicle; Α-tocopherol; Ternatin. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009 |
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| Domingues,S.F.S.; Ferreira,H.S.; Muniz,J.A.P.C.; Lima,A.K.F.; Ohashi,O.M.; Figueiredo,J.R.; Silva,L.D.M.. |
| The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monkey; Preantral follicle; Isolation; Ovary; Cebus apella. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009 |
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| Rodrigues,G.Q.; Lima,I.M.T.; Chaves,R.N.; Rossetto,R.; Costa,S.L.; Castro,S.V.; Barros,V.R.P.; Matos,M.H.T.; Lopes,C.A.P.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.. |
| The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preantral follicle; In vitro development; TGF-β. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200013 |
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