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| Kumar,Namrata; Moreno,Natália C.; Feltes,Bruno C.; Menck,Carlos FM; Houten,Bennett Van. |
| Abstract Base and nucleotide excision repair (BER and NER) pathways are normally associated with removal of specific types of DNA damage: small base modifications (such as those induced by DNA oxidation) and bulky DNA lesions (such as those induced by ultraviolet or chemical carcinogens), respectively. However, growing evidence indicates that this scenario is much more complex and these pathways exchange proteins and cooperate with each other in the repair of specific lesions. In this review, we highlight studies discussing the involvement of NER in the repair of DNA damage induced by oxidative stress, and BER participating in the removal of bulky adducts on DNA. Adding to this complexity, UVA light experiments revealed that oxidative stress also causes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Base excision repair; Nucleotide excision repair; DNA damage; Protein oxidation; UVA light. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200309 |
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| Bouriche,Hamama; Kada,Seoussen; Senator,Abderrahmane; demirtas,Ibrahim; Ozen,Tevfik; Toptanci,Bircan Çeken; Kizil,Goksel; Kizil,Murat. |
| ABSTRACT The protective activity of methanolic (Met E) and aqueous (Aq E) extracts of Globularia alypum L. (G. alypum) against DNA, lipid and protein oxidative damage was investigated. Moreover, the scavenging, chelating, and reducing power activities of the extracts were also evaluated. Phytochemical analysis was performed to determine phenolic compounds. Results showed that Met E and Aq E were rich in phenolic compounds, and were able to scavenge DPPH˙ with IC50 values of 48.61 µg/mL and 51.97 µg/mL, respectively. In addition, both extracts were able to chelate ferrous ions. At 300 μg/mL, the chelating activity was 97.53% and 91.02%, respectively. The reducing power of these extracts was also remarkable and concentration dependent. At 100 µg/mL, both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; DNA damage; Globularia alypum; Lipid peroxidation; Protein oxidation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100422 |
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| Souza,Marta A. A.; Visentainer,Jesui V.; Carvalho,Rafael H.; Garcia,Fabianne; Ida,Elza I.; Shimokomaki,Massami. |
| In this study, the changes in the lipid (Lox) and protein oxidation (Pox) were measured quantitatively by TBARS and carbonyl methods, respectively, throughout the salting and drying steps of charqui meat (CH) and jerked beef (JB) preparation and their storage up to 60 days. The experiment was carried out on CH samples treated with brine (20.0%) and JB with same brine solution added with sodium nitrite (0.02%). After 60 days of storage, the carbonyl substances in CH were 2.77nmol mg-1 while in the JB samples, there was 61.0% oxidation inhibition. The TBARS determination revealed a Lox inhibition by approximately 5-fold in the latter samples. These results indicated that in the metmyoglobin molecule, the nitrite kept the Fe in the Fe2+ state in JB samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Protein oxidation; Charqui; Jerked beef. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100014 |
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| Mendanha,S.A.; Anjos,J.L.V.; Silva,A.H.M.; Alonso,A.. |
| Electron paramagnetic resonance (EPR) spectroscopy of spin labels was used to monitor membrane dynamic changes in erythrocytes subjected to oxidative stress with hydrogen peroxide (H2O2). The lipid spin label, 5-doxyl stearic acid, responded to dramatic reductions in membrane fluidity, which was correlated with increases in the protein content of the membrane. Membrane rigidity, associated with the binding of hemoglobin (Hb) to the erythrocyte membrane, was also indicated by a spin-labeled maleimide, 5-MSL, covalently bound to the sulfhydryl groups of membrane proteins. At 2% hematocrit, these alterations in membrane occurred at very low concentrations of H2O2 (50 µM) after only 5 min of incubation at 37°C in azide phosphate buffer, pH 7.4. Lipid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electron paramagnetic resonance; Spin label; Erythrocyte; Membrane fluidity; Membrane oxidation; Protein oxidation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600001 |
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| Gonçalves,Jamile F.; Becker,Alexssandro G.; Cargnelutti,Denise; Tabaldi,Luciane A.; Pereira,Luciane B.; Battisti,Vanessa; Spanevello,Rosélia M.; Morsch,Vera M.; Nicoloso,Fernando T.; Schetinger,Maria R.C.. |
| In this study, the effects of cadmium (Cd) on lipid peroxidation, electrolyte leakage, protein oxidation, ascorbate peroxidase (APX; E.C. 1.11.1.11), catalase (CAT; E.C. 1.11.1.6) and superoxide dismutase (SOD; E.C. 1.15.1.1) activities, and ascorbic acid, non-protein thiol groups and total soluble protein contents in cucumber seedlings (Cucumis sativus L.) were investigated. Seedlings were grown in vitro in an agar-solidified substrate containing four Cd levels as CdCl2 (0, 100, 400, and 1000 µmol L-1) for 10 d. The lowest Cd level decreased the malondialdehyde concentration. Electrolyte leakage increased only at 1000 µmol Cd L-1, whereas protein oxidation and total soluble protein content were enhanced at 400 and 1000 µmol Cd L-1. Activity of APX was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant enzymes; Ascorbic acid; Cucumis sativus; Heavy metal; Lipid peroxidation; Protein oxidation. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300006 |
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| Gonçalves,Jamile F.; Becker,Alexssandro G.; Pereira,Luciane B.; Rocha,João B. T. da; Cargnelutti,Denise; Tabaldi,Luciane A.; Battisti,Vanessa; Farias,Júlia G.; Fiorenza,Amanda M.; Flores,Érico M. M.; Nicoloso,Fernando T.; Schetinger,Maria R. C.. |
| In this study, the effects of lead (Pb) on growth, photosynthetic pigments concentration, lipid peroxidation, electrolyte leakage percentage (ELP), protein oxidation, aminolevulinate dehydratase (ALA-D; E.C. 4.2.1.24), ascorbate peroxidase (APX; E.C. 1.11.1.11), catalase (CAT; E.C. 1.11.1.6) and superoxide dismutase (SOD; E.C. 1.15.1.1) activities, and ascorbic acid (AsA), non-protein thiol groups (NPSH) and total soluble protein concentrations in cucumber seedlings (Cucumis sativus L.) were investigated. Seedlings were grown in vitro in an agar-solidified substrate containing three Pb levels as (C2H3O2)Pb.3H2O (0, 100, 400, and 1000 µmol L-1) for 10 d. Increasing Pb concentrations in substrate enhanced Pb concentration in both roots and shoot. Pb... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant enzymes; Cucumber; Lipid peroxidation; Pb; Photosynthetic pigments; Protein oxidation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000300002 |
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| Maiti,Saborni; Ghosh,Nirmalia; Mandal,Chiranjib; Das,Kingsuk; Dey,Narottam; Adak,Malay Kumar. |
| Two maize varieties, viz. Sartaj and Deccan, have been evaluated according to their responses to chromium (Cr) in terms of specific physiological and biochemical attributes. During the exposure to Cr concentration (0, 50, 100, 200 and 300 µM) of potassium dichromate (K2Cr2O7) for seven days, significant changes of oxidative stress in terms of lipid peroxidation and protein oxidation were recorded along with other antioxidative pathways. It is revealed that irrespective of varieties, Sartaj was more prone to oxidative stress since the amount of superoxide due to Cr exposure was found to be significant, unlike the second variety, Deccan. In addition, for Sartaj the activity of NAD(P)H oxidase recorded a significant linear increase in a dose response... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidative enzymes; MDA; Protein oxidation; ROS. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000300007 |
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| Piccolo,Jaqueline; Daniel,Ana Paula; Klein,Bruna; Ferreira,Lauren Fresinghelli; Ruviaro,Amanda Roggia; Emanuelli,Tatiana; Kubota,Ernesto Hashime. |
| This study investigated the effects of hydroethanolic E. japonica seed extracts (EJSE) as inhibitors of lipid and protein oxidation on fish pates subjected to refrigerated storage. Five fish pate formulations were developed. These formulations included two control pates (water-control and ascorbic acid-control) and three pates with added EJSE (0.1, 0.2 and 0.4g of seed 100g-1 product, equivalent to 3.4, 6.8 or 13.6mg phenolic compounds kg-1 product), which were then stored under refrigeration for 35 days. Conjugated dienes (CD) and peroxide (PV) values increased along with the storage time; however, these values decreased and were similar among all samples at the end of 35 days of analysis (P<0.05). However, the thiobarbituric acid reactive substances... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eriobotrya japonica; Rhamdia quelen; Lipid oxidation; Protein oxidation; Wastes. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000901705 |
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