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| Beretta,A.L.R.Z.; Trabasso,P.; Stucchi,R.B.; Moretti,M.L.. |
| Methicillin-resistant Staphylococcus aureus (MRSA) has been the cause of major outbreaks and epidemics among hospitalized patients, with high mortality and morbidity rates. We studied the genomic diversity of MRSA strains isolated from patients with nosocomial infection in a University Hospital from 1991 to 2001. The study consisted of two periods: period I, from 1991 to 1993 and period II from 1995 to 2001. DNA was typed by pulsed-field gel electrophoresis and the similarity among the MRSA strains was determined by cluster analysis. During period I, 73 strains presented five distinctive DNA profiles: A, B, C, D, and E. Profile A was the most frequent DNA pattern and was identified in 55 (75.3%) strains; three closely related and four possibly related... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular epidemiology; Hospital infection; Pulsed-field gel electrophoresis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900009 |
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| Darini,A.L.C.; Magalhães,V.D.; Levy,C.L.; Barth,A.L.; Coscina,A.L.. |
| In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-serotyping. Plasmid profile analysis, ribotyping, and macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) were used as genotyping methods. Sero- and phage-typing were not useful since the majority of isolates could not be subtyped by these methods. Biotyping, antibiogram and plasmid profile permitted us to classify the samples into different groups... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Enterobacter cloacae; Phage-typing; Serotyping; Ribotyping; Pulsed-field gel electrophoresis; Nosocomial infection. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900004 |
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| Kacmaz,Birgul; Unaldi,Ozlem; Sultan,Nedim; Durmaz,Riza. |
| The aims of this study were to investigate drug resistance rates, types of extended spectrum beta lactamases (ESBLs), and molecular epidemiological characteristics of 43 Shigella sonnei isolates. Ampicillin-sulbactam, amoxicillin-clavulanate, chloramphenicol, and ciprofloxacin were the most active antibiotics. Five isolates harbored blaSHV-12, blaTEM-1 and blaCTX-M-15. More than 90% of the isolates had an indistinguishable pulsotype. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella sonnei; Extended spectrum beta-lactamase; Plasmid profile; Pulsed-field gel electrophoresis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300013 |
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| Braga,Valeria; Vázquez,Sylvia; Vico,Victoria; Pastorino,Valeria; Mota,María Inés; Legnani,Marcela; Schelotto,Felipe; Lancibidad,Gustavo; Varela,Gustavo. |
| ABSTRACT The aim of this work was to study the prevalence of Listeria monocytogenes in foods obtained in retail shops and food industries located in Montevideo-Uruguay, and to identify the serogroups of the obtained isolates. Three-thousand one-hundred and seventy-five food samples (frozen, deli meats, ready-to-eat and cheese) were analyzed. The obtained isolates were serogrouped by multiplex PCR and serotyped by conventional procedure. Genetic comparisons were performed using pulsed-field gel electrophoresis on a sub-set of isolates belonging to the same serotype successively recovered from the same establishment. L. monocytogenes was isolated from 11.2% of samples. The highest prevalence was observed in frozen foods (38%), followed by cheese (10%). 1/2b... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food-borne diseases; Listeriosis; Serotyping; Pulsed-field gel electrophoresis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400689 |
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