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Registros recuperados: 19
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Crecimiento de cuatro cepas del género Rhizobium en presencia de hidrocarburos policíclicos aromáticos (HPA). Colegio de Postgraduados
González Paredes, Yessica.
Este estudio evaluó el crecimiento de cuatro cepas de Rhizobium en medio contaminado con fenantreno (PHE) o benzo[a]pireno (BaP) en concentraciones de 0, 10, 20, 40, 60, 80 y 100 µg mL-1. En la primera fase experimental, R. tropici CIAT899, R. tropici PRF81, R. leucaena CFN299 y R. etli CFN42 crecieron en condiciones in vitro o en simbiosis con Phaseolus vulgaris L. en presencia de PHE y BaP. El crecimiento in vitro de las cuatro cepas a las 24 h, disminuyó al aumentar las concentraciones de ambos HPA; sin embargo, el BaP fue más tóxico para su crecimiento. Las cuatro cepas ante 80 y 100 µg BaP mL-1 presentaron una fase de adaptación; para CIAT899 y PRF81 esta fase fue de 48 h, y para CFN299 y CFN42 esta fase fue de 72 y 96 h, respectivamente. La simbiosis...
Palavras-chave: Rizobios; Tolerancia; Adaptación; Simbiosis; Degradación; Fenantreno; Benzol [a] pireno; Rhizobia; Tolerance; Adaptation; Symbiosis; Degradation; Phenanthrene; Benzol [a] pyrene; Edafología; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/1704
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Aislamiento y evaluación del potencial biofertilizante de bacterias aplicables en el desarrollo agrícola sostenible en Chihuahua. Colegio de Postgraduados
Hernández Flores, Lina.
El impacto ecológico es importante en los sistemas de producción y el uso de productos amigables con el medio ambiente es elemental para preservar y mantener los recursos naturales. Así, la aplicación de biotecnologías, como el uso de microorganismos benéficos, permite sustituir de manera parcial o total la fertilización nitrogenada. Para ello, es importante la adecuada selección de microorganismos aplicables a diferentes cultivos y condiciones edafoclimáticas como fertilizantes biológicos. El interés de desarrollar una investigación acerca de estas bacterias es el de poder utilizarlas eficientemente en la agricultura. Por ello, se aislaron cepas bacterianas de nódulos de leguminosas y de suelo con los cultivos económicamente más importantes en comunidades...
Palavras-chave: Agricultura sustentable; Biofertilizantes; Nódulos; Rhizobia; Suelo; Sustainable agriculture; Biofertilizante; Nodules; Rhizobia; Soil; EDAR; Estrategias para el Desarrollo Agrícola Regional; Doctorado.
Ano: 2011 URL: http://hdl.handle.net/10521/619
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Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants Genet. Mol. Biol.
Granada,Camille E; Strochein,Marcos; Vargas,Luciano K; Bruxel,Manuela; Sá,Enilson Luiz Saccol de; Passaglia,Luciane M.P.
This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizobia; Nodulation; Nitrogen; Specificity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300012
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Isolamento e caracterização fenotípica de rizóbios obtidos de substrato de cupinzeiro, utilizando-se soja como planta-isca. Infoteca-e
SILVA, A. T.; PORTILHO, I. I. R.; COSTA, M. R.; MERCANTE, F. M..
bitstream/item/69052/1/063-silva-isolamento.pdf
Tipo: Outras publicações técnicas (INFOTECA-E) Palavras-chave: Phenotipic; Rhizobia; Trap-host.
Ano: 2012 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/938542
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Role of Rhizobia in Suppressing the Root Diseases of Soybean Under Soil Amendment Planta Daninha
PARVEEN,G.; NOREEN,R.; SHAFIQUE,H.A.; SULTANA,V.; EHTESHAMUL-HAQUE,S.; ATHAR,M..
ABSTRACT: Rhizobia are soil bacteria, characterized by their unique ability to colonize the roots of leguminous crops, where they form nitrogen fixing nodules. Considerable evidence has been accumulated to identify the benefits associated with use of rhizobia as biocontrol agents against soil-borne pathogens, in addition to biological nitrogen fixation. In this study, out of four rhizobial isolates tested, Bradyrhizobium sp. inhibited the radial growth of all the test fungi viz: Macrophomina phaseolina, Rhizoctonia solani, Fusarium solani and F. oxysporum, while other 3 rhizobial isolates inhibited the growth of at least three fungi. Cell free culture filtrates of rhizobia also showed nematicidal activity by killing second-stage juveniles of Meloidogyne...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mustard cake; Rhizobia; Soybeans.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100237
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Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans Rev. Bras. Ciênc. Solo
Barbosa,Leonardo de Paiva; Costa,Patrícia Freitas; Ribeiro,Paula Rose Almeida; Rufini,Márcia; Guimarães,Amanda Azarias; Moreira,Fatima Maria de Souza.
ABSTRACT High yields obtained from soybean [Glycine max (L.) Merrill], and consequent profits, are related to efficient symbiosis of soybean with nitrogen fixing bacteria of the Bradyrhizobium genus. Inoculation with strains belonging to the Bradyrhizobium japonicum, B. elkanii, and B. diazofficiens species, approved by the Brazilian Ministry of Agriculture (MAPA), is a common practice for this crop. However, variations in the composition of inoculants can change the quality of the product. The aim of this study was to genetically identify and to evaluate the symbiotic efficiency of isolates with cultural characteristics different from those of strains approved by MAPA and which are present in two commercial inoculants for soybean. Isolates obtained from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; 16S rRNA gene; Cultural characterization; Rhizobia; Genetic diversity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100432
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Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil Rev. Bras. Ciênc. Solo
Menezes,Kelly Alexsandra Souza; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Martins,Lindete Míria Vieira; Fernandes Júnior,Paulo Ivan.
ABSTRACT Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from “mulungu” (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological nitrogen fixation; “mulungu”; Tree legumes; “Caatinga”; Rhizobia.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100404
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Exopolysaccharides produced by the symbiotic nitrogen-fixing bacteria of leguminosae Rev. Bras. Ciênc. Solo
Bomfeti,Cleide Aparecida; Florentino,Ligiane Aparecida; Guimarães,Ana Paula; Cardoso,Patrícia Gomes; Guerreiro,Mário César; Moreira,Fatima Maria de Souza.
The process of biological nitrogen fixation (BNF), performed by symbiotic nitrogen fixing bacteria with legume species, commonly known as α and β rhizobia, provides high sustainability for the ecosystems. Its management as a biotechnology is well succeeded for improving crop yields. A remarkable example of this success is the inoculation of Brazilian soybeans with Bradyrhizobium strains. Rhizobia produce a wide diversity of chemical structures of exopolysaccharides (EPS). Although the role of EPS is relatively well studied in the process of BNF, their economic and environmental potential is not yet explored. These EPS are mostly species-specific heteropolysaccharides, which can vary according to the composition of sugars, their linkages in a single...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Exopolysaccharides; Nodules; Environmental stress; Soil aggregation; Rhizobia.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000300001
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Land use and vegetation cover on native symbionts and interactions with cowpea AGRIAMBI
Rocha,Beatriz C. F.; Santos,Enésia O. da S.; Santos,José G. D.; Takako,Adriana K.; Castro,Fabio J..
ABSTRACT Arbuscular mycorrhizal fungi and rhizobia are important components of agroecosystems and they respond to human interference. The objective of this study was to investigate native communities of those microorganisms in soil collected under the native forest, four pastures (Brachiaria brizantha, Panicum maximum, Arachis pintoi and Stylosanthes guianensis) and a fallow soil after maize cultivation, in interaction with cowpea (Vigna unguculata). The cowpea grew in a greenhouse until flowering. They were randomly distributed depending on soil, in five replications. The lowest mycorrhizal fungi sporulation and mycorrhizal root colonization occurred under the Panicum and forest soil. In the soils under forest and Stylosanthes, the cowpea did not exhibit...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arbuscular mycorrhiza; Rhizobia; Legal Amazon; Nodulation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662017000200116
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Molybdenum supply and biological fixation of nitrogen by two Brazilian common bean cultivars AGRIAMBI
Silva,Alinne da; Franzini,Vinícius I.; Piccolla,Cristiano D.; Muraoka,Takashi.
ABSTRACT The common bean has been considered to have low biological nitrogen fixation capacity; however, this process can be made more effective with molybdenum (Mo) supplementation. The objective of this study was to evaluate the influence of Mo rates on the growth and biological nitrogen fixation by two Brazilian common bean cultivars using the 15N isotope dilution technique. The experiment was performed in 2014 in a completely randomized design arranged in a 5 x 3 factorial scheme, corresponding to 5 rates of Mo (control, 40, 80, 120 and 240 g ha-1), the common bean cultivars Aporé, Ouro Negro and NORH-54 (a non-nodulating common bean cultivar), and three replicates. The application of Mo and the inoculation with rhizobia strains contributed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isotope dilution; Labeled fertilizer; Rhizobia.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662017000200100
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Quantitative evaluation of acidity tolerance of root nodule bacteria Rev. Microbiol.
Oliveira,Luiz Antonio de; Magalhães,Hélio Paracaima de.
Quantification of acidity tolerance in the laboratory may be the first step in rhizobial strain selection for the Amazon region. The present method evaluated rhizobia in Petri dishes with YMA medium at pH 6.5 (control) and 4.5, using scores of 1.0 (sensitive, "no visible" growth) to 4.0 (tolerant, maximum growth). Growth evaluations were done at 6, 9, 12, 15 and 18 day periods. This method permits preliminary selection of root nodule bacteria from Amazonian soils with statistical precision. Among the 31 rhizobia strains initially tested, the INPA strains 048, 078, and 671 presented scores of 4.0 at both pHs after 9 days of growth. Strain analyses using a less rigorous criterion (growth scores higher than 3.0) included in this highly tolerant group the INPA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizobia; Tolerance to pH; Amazon.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300004
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Growth promotion of common bean and genetic diversity of bacteria from Amazon pastureland Scientia Agricola
Ferreira,Linnajara de Vasconcelos Martins; Carvalho,Fernanda de; Andrade,Júlia Fonseca Colombo; Moreira,Fatima Maria de Souza.
ABSTRACT: A significant number of bacterial species, particularly in the rhizosphere, may benefit plant growth and development. This group of bacteria is known as plant growth-promoting rhizobacteria (PGPR). This study identified genetically isolates of common bean nodules used to trap bacteria from Amazon pastureland and investigated their capacity of nodulating and promoting growth of common bean when inoculated or co-inoculated with CIAT899 strain (Rhizobium tropici). Two experiments were carried out in a greenhouse, in axenic conditions, using the common bean cultivar Talismã. In the first experiment, 56 PGPR strains were evaluated individually regarding growth promotion and nodulation. In the second experiment, inoculation of seven PGPR strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Co-inoculation; Endophytic bacteria; Rhizobia.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000600461
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Symbiotic efficiency and genetic characteristics of Bradyrhizobium sp. strain UFSM LA 1.3 isolated from Lupinus albescens (H. et Arn) Scientia Agricola
Stroschein,Marcos Roberto Dobler; Eltz,Flávio Luiz Foletto; Antoniolli,Zaida Inês; Lupatini,Manoeli; Vargas,Luciano Kaiser; Giongo,Adriana; Pontelli,Mateus Padoin.
Legume species belonging to the genus Lupinus are annual herb plants. The majority of them are indigenous to the Americas. They are known for nitrogen-fixing symbioses with soil bacteria collectively called rhizobia. The aim of this study was to characterize a rhizobium strain isolated from Lupinus albescens using phenotypic, symbiotic and molecular approaches. Strain UFSM LA 1.3 was tested in vitro according to several parameters: colony size, color and growing rate; acid or alkaline reaction in yeast mannitol media supplemented with bromothymol blue; gum production. Molecular characterization was evaluated by PCR technique using primers BOX A1-R and sequence analysis of the 16S-23S rDNA intergenic region (ITS). ITS sequencing fragments showed genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lupin; Rhizobia; Genotypic approaches.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600012
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Agronomic efficiency of Rhizobium strains from the Amazon region in common bean Acta Amazonica
NOGUEIRA,Cláudia de Oliveira Gonçalves; OLIVEIRA,Dâmiany Pádua; FERREIRA,Paulo Ademar Avelar; PEREIRA,João Paulo de Andrade Rezende; VALE,Helson Mário Martins do; ANDRADE,Messias José Bastos de; MOREIRA,Fatima Maria de Souza.
ABSTRACT To maximize the contribution of biological nitrogen fixation in common bean, it is necessary to use bacterial strains that are more adapted, competitive, and efficient in the symbiotic process. In this regard, the aim of this study was to evaluate the agronomic efficiency (AE) of three bacterial strains isolated from acid soils with high Al content from the Amazon region in an Argissolo Vermelho Distrófico típico soil (Typic Rhodustults - USDA Classification) from the municipality of Formiga, MG, Brazil. We compared their AE to that of the reference strain CIAT 899T and of two controls without inoculation (one without and another with 80 kg ha-1 of N-urea). The results indicated that inoculation with the strains UFLA 02-100 and UFLA 02-127...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Phaseolus vulgaris L.; Rhizobia; Biological nitrogen fixation; Seed inoculation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000300273
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Nodulation, arbuscular mycorrhizal colonization and growth of some legumes native from Brazil Acta Botanica
Patreze,Camila M.; Cordeiro,Lázara.
The effects of rhizobial and mycorrhizal inoculation, nitrogen and phosphorus fertilization on nodulation, mycorrhizal colonization and initial growth were examined in Brazilian native plants, Enterolobium contortisiliquum (Vell. Conc.) Morong, Inga laurina (Sw.) Willd., Lonchocarpus muehlbergianus Hassl and Platypodium elegans Vogel. The experiment was carried out in a glasshouse using plastic bags filled with a mixture of sandy soil from riparian forest and vermiculite (2:1) amended with basal nutrients including NP, P and N and infected with rhizobia (r), mycorrhiza (m) or both (rm), amounting seven treatments: NP, P, P+r, P+rm, N, N+m and N+rm, with ten replications each. The plants were analyzed at 120 and 255 days after sowing. P deficiency...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizobia; Dual inoculation; Enterolobium contortisiliquum; Inga laurina; Lonchocarpus muehlbergianus; Platypodium elegans.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062005000300014
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Rhizobial characterization in revegetated areas after bauxite mining BJM
Borges,Wardsson Lustrino; Prin,Yves; Ducousso,Marc; Le Roux,Christine; Faria,Sergio Miana de.
Abstract Little is known regarding how the increased diversity of nitrogen-fixing bacteria contributes to the productivity and diversity of plants in complex communities. However, some authors have shown that the presence of a diverse group of nodulating bacteria is required for different plant species to coexist. A better understanding of the plant symbiotic organism diversity role in natural ecosystems can be extremely useful to define recovery strategies of environments that were degraded by human activities. This study used ARDRA, BOX-PCR fingerprinting and sequencing of the 16S rDNA gene to assess the diversity of root nodule nitrogen-fixing bacteria in former bauxite mining areas that were replanted in 1981, 1985, 1993, 1998, 2004 and 2006 and in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizobia; Rep-PCR; Cluster analysis; Nodules; Mining.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200314
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Genomic identification and characterization of the elite strains Bradyrhizobium yuanmingense BR 3267 and Bradyrhizobium pachyrhizi BR 3262 recommended for cowpea inoculation in Brazil BJM
Leite,Jakson; Passos,Samuel Ribeiro; Simões-Araújo,Jean Luiz; Rumjanek,Norma Gouvêa; Xavier,Gustavo Ribeiro; Zilli,Jerri Édson.
ABSTRACT The leguminous inoculation with nodule-inducing bacteria that perform biological nitrogen fixation is a good example of an “eco-friendly agricultural practice”. Bradyrhizobium strains BR 3267 and BR 3262 are recommended for cowpea (Vigna unguiculata) inoculation in Brazil and showed remarkable responses; nevertheless neither strain was characterized at species level, which is our goal in the present work using a polyphasic approach. The strains presented the typical phenotype of Bradyrhizobium with a slow growth and a white colony on yeast extract-mannitol medium. Strain BR 3267 was more versatile in its use of carbon sources compared to BR 3262. The fatty acid composition of BR 3267 was similar to the type strain of Bradyrhizobium yuanmingense;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vigna unguiculata; Rhizobia; MLSA; DDH; ANI.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400703
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Symbiotic potential and survival of native rhizobia kept on different carriers BJM
Ruíz-Valdiviezo,Víctor Manuel; Canseco,Lucía María Cristina Ventura; Suárez,Luis Antonio Castillo; Gutiérrez-Miceli,Federico Antonio; Dendooven,Luc; Rincón-Rosales,Reiner.
Native rhizobia are ideal for use as commercial legume inoculants. The characteristics of the carrier used to store the inoculants are important for the survival and symbiotic potential of the rhizobia. The objective of this study was to investigate the effects of peat (PEAT), perlite sugarcane bagasse (PSB), carboxymethyl cellulose plus starch (CMCS), and yeast extract mannitol supplemented with mannitol (YEMM) on the survival, nodulation potential and N2 fixation capacity of the native strains Sinorhizobium mexicanum ITTG R7T and Rhizobium calliandrae LBP2-1T and of the reference strain Rhizobium etli CFN42T. A factorial design (4 × 3) with four repetitions was used to determine the symbiotic potential of the rhizobial strains. The survival of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizobia; Inoculants; Nodulation; Nitrogen fixation; Carrier.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300735
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Triple inoculation with Bradyrhizobium, Glomus and Paenibacillus on cowpea (Vigna unguiculata [L.] walp.) development BJM
Lima,André Suêldo Tavares de; Xavier,Terezinha Ferreira; Lima,Cláudia Elizabete Pereira de; Oliveira,José de Paula; Mergulhão,Adália Cavalcanti do Espírito Santo; Figueiredo,Márcia do Vale Barreto.
The use of microorganisms to improve the availability of nutrients to plants is of great importance to agriculture. This study aimed to evaluate the effect of triple inoculation of cowpea with arbuscular mycorrhizal fungi (AMF), plant growth-promoting bacteria (PGPB) and rhizobia to maximize biological nitrogen fixation (BNF) and promote plant growth. The experiment was conducted in a greenhouse using cowpea plants (Vigna unguiculata L. Walp cv. IPA 206). The treatments included inoculation with strains of Bradyrhizobium sp. (BR 3267 and EI - 6) individually and as a mixture, an absolute control (AC) and mineral nitrogen control (NC), all combined with the presence or absence of native AMF (Glomus etunicatum) and PGPB (Paenibacillus brasilensis - 24) in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arbuscular mycorrhiza; Biological nitrogen fixation; PGPB; Rhizobia.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300010
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