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| Registros recuperados: 16 | |
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| Lourenço,Luciana B.; Garcia,Paulo C.A.; Recco-Pimentel,Shirlei M.. |
| Physical maps of the ribosomal RNA gene 28S of species belonging to the genera Paratelmatobius and Scythrophrys were constructed, using five restriction endonucleases. The restriction sites for Bam HI, Bgl II, Bst EII, and Eco RI had similar positions in all species, although there were interspecific differences in the size of the restriction fragments obtained. An additional Pvu II site was found in Scythrophrys specimens from Piraquara (State of Paraná, Brazil) and from São Bento do Sul (State of Santa Catarina, Brazil), but not in the Scythrophrys specimens from Rancho Queimado (State of Santa Catarina, Brazil). This finding is in agreement with the hypothesis regarding the existence of two species in the genus Scythrophrys. On the other hand, the extra... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ribosomal DNA; 28S rDNA gene; Restriction sites; Physical map; Paratelmatobius; Scythrophrys; Leptodactylidae; Anura. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200006 |
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| Nakayama,Celeste M.; Feldberg,Eliana; Bertollo,Luiz Antonio C.. |
| Karyotypic characteristics of three species of the genus serrasalmus ( S. altispinnis , S. gouldingi and S. Serrulatus ) from the middle and lower Negro River, Amazon Basin, were investigated using different staining techniques and Fluorescent in situ hybridization with 5S and 18S rDNA probes. The diploid number was invariably 2n = 60 and the fundamental number was FN = 110. Nevertheless, the karyotypes differed from each other in composition: 24m, 20sm, 6st, 10a in S. altispinnis ; 22m, 22sm, 6st, 10a in S. gouldingi and 20m, 22sm, 8st, 10a in S. serrulatus. The karyotype of S. altispinnis differed from the one previously described in a population from the Pitinga River. C-positive constitutive heterochromatin was mainly pericentromeric in... |
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Palavras-chave: Chromosome banding; Cytotaxonomy of piranhas; FISH; Karyotype; Ribosomal DNA. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500011 |
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| Pires,Larissa Bettin; Usso,Mariana Campaner; Giuliano-Caetano,Lucia; Dias,Ana Lúcia. |
| Abstract The genera Cichlasoma and Gymnogeophagus belong to the subfamily Cichlinae, the only one in Neotropical cichlids. Cichlasoma dimerus, C. paranaense, C. portalegrense, Gymnogeophagus rhabdotus, and G. lacustris were collected at different points in the Paranapanema and Paraguay basins and the Lagoon of Patos hydrographic system. In addition to conventional analysis, CMA3 fluorochrome staining, and FISH with 18S rDNA probe were performed. All species had a diploid number equal to 48, with interand intraspecific differences in karyotype formulae. All species presented a single AgNOR site, except G. rhabdotus and the C. paranaense population of the Paranapanema River, which revealed more than one pair of nucleolar chromosomes. AgNORs were coincident... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome banding; Fish cytogenetics; Karyotype diversification; Ribosomal DNA. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400203 |
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| Castrillo,M.L; Fonseca,M.I; Bich,G.A; Jerke,G; Horianski,M.A; Zapata,P.D. |
| Members of Aspergillus section Nigri are distributed worldwide and are potentially ochratoxin A producers; some of them are used at industrial level as source of extracellular enzymes and organic acids. The taxonomy of this section comprises one of the most complex and confusing of genus Aspergillus. The objectives of present work were to study the phylogeny of Aspergillus section Nigri and their taxonomy by amplifying ITS1, 5.8S and ITS2 regions. We analyzed 14 strains of Aspergillus section Nigri isolated from different commercial forms of yerba mate, classified according to morphology. Genomic DNA was extracted, the ITS regions were amplified with universal primers ITS1 and ITS4, and the obtained fragments were sequenced. The obtained sequences were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus section Nigri; Taxonomy; Ribosomal DNA. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332012000300003 |
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| Almeida,Raul P. de; Stouthamer,Richard. |
| Discovery of male morphological characters for species identification was a great improvement in Trichogramma systematic. However, not all species could be easily identified. In some cases, the lack of males (thelytokous status of species that carry the Wolbachia symbiont) made Trichogramma identification impossible. This problem was solved via antibiotic and heating treatments for elimination of the bacteria and allowing the production of males. The only Trichogramma species reported in which thelytoky is not induced by bacterial infection is T. cacoeciae, so here another means of species identification is needed. This species was identified based on the ITS2 (Internal transcribed spacer 2) sequence, a modern technique that has been proved useful in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Parasitoid; Thelytoky; Ribosomal DNA. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000200011 |
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| Marajó,Leandro; Viana,Patrik F.; Ferreira,Milena; Py-Daniel,Lúcia H. Rapp; Feldberg,Eliana. |
| ABSTRACT Farlowella is one of the most diverse genera of the Loricariinae, restricted to South America rivers. The taxonomic and phylogenetic relationships among its species are contentious and, while genetic studies would contribute to the understanding of their relationships, the only available datum refer to the karyotype description of only one species. In the present study two Amazonian species, Farlowella cf. amazonum and F. schreitmuelleri, were analyzed using conventional and molecular cytogenetic procedures. Both species had diploid chromosome number 58, but different fundamental numbers (NF) 116 and 112, respectively, indicative of chromosomal rearrangements. C-banding is almost poor, especially in F. cf. amazonum, and occurs predominantly in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon region; Chromosomal markers; Karyotypic characterization; Ribosomal DNA; Twig catfish. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000400213 |
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| Magnani,Marciane; Fernandes,Thiago; Prete,Cássio Egidio Cavenaghi; Homechim,Martin; Ono,Elisabete Yurie Sataque; Vilas-Boas,Laurival Antonio; Sartori,Daniele; Furlaneto,Márcia Cristina; Fungaro,Maria Helena Pelegrinelli. |
| Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ITS; Ribosomal DNA; Ochratoxin; Mycotoxin. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100009 |
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| Chiarini,Franco E; Gauthier,Martha J. |
| The chromosomes of Solanum nelsonii, S. sandwicense (endemic to the Hawaii islands) and S. vespertilio (from the Canary islands) were studied by means of classical staining, CMA/DAPI banding and FISH with probes for the 18-5.8-26S and 5S rDNA genes. The aim of this study was to test for chromosomal changes (chromosome number, karyotype, heterochromatin pattern, rDNA loci) during the evolution of these taxa with respect to their continental relatives. An apparent chromosome stasis was confirmed in all three species, in regards to chromosome number and karyotype morphology. However, there was also evidence of cryptic, cumulative sequence changes. Speciation in these species is not likely associated with large, obvious chromosome rearrangements or polyploidy,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome stasis; Heterochromatin; Ribosomal DNA; Solanum subgen; Leptostemonum. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000200009 |
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| Pinto,Flavia Cristina Jastale; Lima,Daniel Braga de; Agustini,Bruna Carla; Dallagassa,Cibelle Borba; Shimabukuro,Maria Fernanda; Chimelli,Márcio; Brand,Debora; Fadel-Picheth,Cyntia Maria Telles; Bonfim,Tania Maria Bordin. |
| Seven fungi were isolated from 50 samples of cosmetic powders. Morphological analyses and ribosomal DNA Internal Transcribed Spacers sequencing were performed which allowed the discrimination of the isolated fungi as Aspergillus fumigatus, Penicillium sp., and Cladosporium sp. which could have, among their species, potentially pathogenic microorganisms. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial contamination; Ribosomal DNA; Cosmetics. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600013 |
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| Registros recuperados: 16 | |
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