SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Miossec, Laurence; Le Guyader, Soizick; Haugarreau, Larissa; Comps, Marie-annick; Pommepuy, Monique.

Several outbreaks of gastroenteritis related to the consumption of shellfish (frequently eaten raw) have been reported in different parts of the world. In Europe, hum an calicivirus infections may have been involved in winter outbreaks in recent years, although there is little evidence confirming such viral contamination in shellfish. This study presents the first results of a field survey on viral contamination in two shelllish harvesting areas along the French Mediterranean coast. The first, consisting mainly of oyster beds, was c1assified in category A, as determined by fecal coliform counts in shellfish (European Community Directive 91/492), and the second, a mussel bed, was c1assified in category C. Shelllish samples were collected monthly between...

Tipo: Text

Palavras-chave: Shellfish; Viral contamination; Enterovirus; Human calicivirus; Rotavirus; Astrovirus; Fecal coliforms; Gastroenteritis.

Ano: 1998

URL: https://archimer.ifremer.fr/doc/00001/11257/7802.pdf

Devenir des virus entériques humains en milieu marin : Apport des VLPs (Virus Like Particles) pour la purification des coquillages

Rotavirus VLP2/6: a new tool for tracking rotavirus in the marine environment

Loisy, Fabienne.

This work was developed following two research axes: the improvement of norovirus (human and animal strain) and rotavirus detection, and the evaluation of VLPs as a viral substitute for studying their comportment in marine environment. The main point concerning the method optimisation was the development of real-time RT-PCR for norovirus detection. The second part of the work has demonstrated the potential of norovirus and rotavirus VLPs as a viral surrogate as proven by the study of their stability in seawater. As VLPs are non-pathogen particles, the long persistence of rotavirus VLPs in shellfish has been shown in natural conditions (oysters on marine estuaries). The studies of Norwalk virus VLPs attachment to oyster tissues have demonstrated a specific...

Tipo: Text

Palavras-chave: Specific binding; Persistence; Shellfish; Real time RT PCR; VLPs; Rotavirus; Norovirus; Fixation spécifique; Persistance; Coquillage; RT PCR en temps réel; VLPs; Rotavirus.

Ano: 2004

URL: http://archimer.ifremer.fr/doc/2004/these-327.pdf

Viral contamination and depuration of oysters under field conditions

Loisy, Fabienne; Atmar, Robert L.; Cohen, Jean; Bosch, Albert; Le Guyader, Soizick.

The potential of rotavirus 2/6-virus-like-particles (VLP2/6) for use as tracers in the marine environment was investigated. The stability of bovine rotavirus (strain RF) and VLP2/6 in natural seawater at 25degreesC for six days was studied. ELISA and western blot methods were used to quantify the particles. The rates of decline of rotavirus particles and VLP2/6 were similar (approximately 0.5 log 10 per day). Western blot analysis showed that the integrity of capsid proteins VP2 and VP6 was conserved during the incubation time. These results demonstrate that VLP2/6 particles have the same stability in seawater as rotavirus particles. Thus, VLP2/6 can be used as a tracer, which should be of particular value for studying the fate of rotavirus particles in...

Tipo: Text

Palavras-chave: Stability; Tracer; Seawater; Virus like particles; Rotavirus.

Ano: 2004

URL: https://archimer.ifremer.fr/doc/2004/publication-330.pdf

Contamination de l'environnement littoral par les rotavirus du groupe A. Détection par amplification enzymatique en chaîne et analyse du polymorphisme de restriction de séquences virales dans les eaux de surfaces et les coquillages

Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L.

Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine...

Tipo: Text

Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish.

Ano: 1992

URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf

Virus Type-Specific Removal in a Full-Scale Membrane Bioreactor Treatment Process

Dubois, Eric.

Les rotavirus font partie des principaux virus entériques humains susceptibles d'être rencontrés dans l'environnement. Leur mise en évidence par culture cellulaire dans des prélèvements est longue et peu sensible, ce qui explique le manque de données sur la contamination du littoral. La RT-seminested PCR a été développée pour pallier ces inconvénients. Après une mise au point méthodologique cette technique a été utilisée lors d'études in vitro et in situ. Les études in vitro sur le devenir des rotavirus en eau de mer, suivi par RT-PCR quantitative et par multiplication en culture cellulaire, montrent que dans des conditions proches de celles du milieu naturel (eau de mer non stérile et à une température relativement basse), la détection d'ARN viral peut...

Tipo: Text

Palavras-chave: Rotavirus; Contamination; Coquillages; Eaux de stations d'épuration; Echantillons cliniques; RT-PCR; RFLP; Epidémiologie moléculaire.

Ano: 1992

URL: http://archimer.ifremer.fr/doc/00442/55376/56894.pdf

Miura, Takayuki; Schaeffer, Julien; Le Saux, Jean-claude; Le Mehaute, Philippe; Le Guyader, Soizick.

We investigated removal of noroviruses, sapoviruses, and rotaviruses in a full-scale membrane bioreactor (MBR) plant by monitoring virus concentrations in wastewater samples during two gastroenteritis seasons and evaluating the adsorption of viruses to mixed liquor suspended solids (MLSS). Sapoviruses and rotaviruses were detected in 25% of MBR effluent samples with log reduction values of 3- and 2-logs in geometric mean concentrations, respectively, while noroviruses were detected in only 6% of the samples. We found that norovirus and sapovirus concentrations in the solid phase of mixed liquor samples were significantly higher than in the liquid phase (P < 0.01, t test), while the concentration of rotaviruses was similar in both phases. The efficiency...

Tipo: Text

Palavras-chave: Norovirus; Sapovirus; Rotavirus; MBR; Mixed liquor suspended solids; Adsorption.

Ano: 2018

URL: https://archimer.ifremer.fr/doc/00414/52548/53441.pdf

A large multi-pathogen waterborne community outbreak linked to faecal contamination of a groundwater system, France, 2000

Rotavirus gastroenteritis in a children's hospital specialized in craniofacial malformations

Gallay, A; De Valk, H; Cournot, M; Ladeuil, B; Hemery, C; Castor, C; Bon, F; Megraud, F; Le Cann, Pierre; Desenclos, Jc.

A large waterborne outbreak of infection that occurred during August 2000 in a local community in France was investigated initially via a rapid survey of visits to local physicians. A retrospective cohort study was then conducted on a random cluster sample of residents. Of 709 residents interviewed, 202 (28.5%) were definite cases (at least three liquid stools/day or vomiting) and 62 (8.7%) were probable cases (less than three liquid stools/day or abdominal pain). Those who had drunk tap water had a three-fold increased risk for illness (95% CI 2.4-4.0). The risk increased with the amount of water consumed (chi-square trend: p < 0.0001). Bacteriological analyses of stools were performed for 35 patients and virological analyses for 24 patients....

Tipo: Text

Palavras-chave: Waterborne outbreak; Rotavirus; Norovirus; Gastroenteritis; Epidemiology; Campylobacter coli.

Ano: 2006

URL: http://archimer.ifremer.fr/doc/2006/publication-1696.pdf

J. Venom. Anim. Toxins incl. Trop. Dis.

Vieira,NA; Pereira,PCM; Carvalho,LR.

This study aimed to verify the relationship between acute diarrhea provoked by rotavirus and different indicators of craniofacial malformations. In the Hospital for Rehabilitation of Craniofacial Anomalies, 8,724 children with cleft lip and cleft palate were divided into the following groups: acute diarrhea and infection due to rotavirus (C1, n = 62), acute diarrhea (C2, n = 153) and without acute diarrhea (C3, n = 8,509). In C1, 29.03% of the cases consisted of hospital infections associated with the hospitalization period while 38.71% of the patients were aged less than six months. The percentage of children not having breastfed was significantly higher in acute diarrhea groups. Additionally, there was a seasonal prevalence of rotavirus infection between...

Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil

Palavras-chave: Rotavirus; Gastroenteritis; Diarrhea; Child.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200015

Rev. Bras. Ciênc. Avic.

Guerreiro,AN; Moraes,CCG; Marinho,ANR; Barros,BCV; Bezerra,DAM; Bandeira,RS; Silva,RR; Rocha,DCC; Meneses,AMC; Luz,MA; Paz,GS; Mascarenhas,JDP.

ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal...

Rotavirus detection and isolation from chickens with or without symptoms

Palavras-chave: Animals; Amazônia; Picobirnavirus; Reovirus; Rotavirus; RT-PCR..

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100161

Rev. Bras. Ciênc. Avic.

Villarreal,LYB; Uliana,G; Valenzuela,C; Chacón,JLV; Saidenberg,ABS; Sanches,AA; Brandão,PE; Jerez,JA; Ferreira,AJP.

Rotaviruses have been identified as one of the main etiological agents of diarrhea and enteritis in mammals, including humans, and in avian species. Few studies have been published about enteric viruses in Brazilian poultry, including those related to rotavirus infection. Such studies demonstrate significant occurrence and the importance of enteric viruses in poultry presenting intestinal problems. Enteric viruses are the primary cause of injuries to the gut, allowing other agents, especially bacteria, to attach, to penetrate, and to replicate in the enteric tissue, leading to further damage. The aim of the present study was to detect rotavirus in the intestinal contents of layers and broilers by polyacrylamide gel electrophoresis (PAGE) and virus...

Genotipificación de Rotavirus en niños menores de cinco años en la región sur del Ecuador

Palavras-chave: Enteric viruses; Rotavirus; Isolation; MA104 cells; Chicken.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000300009

ABCL

Simaluiza-Masabanda,Rosa Janneth; Cevallos-Solórzano,Gabriela; Bourdett-Stanziola,Lurys; Arévalo-Jaramillo,Paulina; Ruiz-Ramón,Jeimmy; Villacis-Villa,Diana; Torres-Bailón,Paula C; Fernández Jaramillo,Heriberto.

Fueron estudiadas, para la búsqueda de rotavirus, 341 muestras fecales obtenidas de sendos niños menores de cinco años con diarrea aguda, atendidos en dos centros de salud de la ciudad de Loja - Ecuador. De ellas, 56 (16%) fueron positivas para rotavirus, siendo genotipificadas 33 muestras para la determinación de genotipos G y P. Los genotipos más frecuentes fueron G4 (42%), P[6] (36%) y la combinación mixta G4+9P[6] (21%). Este es el primer estudio de carácter molecular en rotavirus realizado en el sur de Ecuador y los datos obtenidos ratifican la variabilidad de cepas de rotavirus circulantes en este país.

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572016000400015

Palavras-chave: Rotavirus; Genotipos; Ecuador.

Ano: 2016

The in vitro comparative cytopathology of a porcine rotavirus and the simian prototype (SA-11)

Arq. Bras. Med. Vet. Zootec.

Lonien,S.C.H.; Bolognini,A.M.; Linhares,R.E.C.; Nozawa,C.M..

A citopatologia in vitro de uma cepa de rotavírus porcino adaptado em cultura de células foi comparada à estirpe-protótipo símia (SA-11). O efeito citopático (ECP) produzido pelos vírus foi semelhante embora a estirpe porcina tivesse apresentado algumas alterações diferentes, como o acentuado estreitamento do citoplasma, com grande perda do volume citoplasmático. O vírus porcino apresentou menor número de plaques de ECP porém com diâmetro maior em relação ao vírus símio, demonstrando maior capacidade de disseminação célula-célula, quase oito vezes mais, a julgar pelo diâmetro dos plaques de ECP. Os elementos do citoesqueleto das células infectadas revelaram uma reorganização semelhante para ambas as estirpes, não sendo possível observar nenhuma diferença,...

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Rotavirus; Cytopathology; Cell culture; Plaque; Cytoskeleton.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400002

Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein

Biol. Res.

VÁSQUEZ-DEL CARPIÓ,RODRIGO; MORALES,JAIME L; BARRO,MARIO; RICARDO,ALBA; SPENCER,EUGENIO.

Rotaviruses are the major cause of acute gastroenteritis in infants world-wide. The genome consists of eleven double stranded RNA segments. The major segment encodes the structural protein VP1, the viral RNA-dependent RNA polymerase (RdRp), which is a minor component of the viral inner core. This study is a detailed bioinformatic assessment of the VP1 sequence. Using various methods we have identified canonical motifs within the VP1 sequence which correspond to motifs previously identified within RdRps of other positive strand, double-strand RNA viruses. The study also predicts an overall structural conservation in the middle region that may correspond to the palm subdomain and part of the fingers and thumb subdomains, which comprise the polymerase core of...

Tipo: Journal article

Palavras-chave: Rotavirus; Genome replication; RNA polymerase; Bioinformatic analysis.

Ano: 2006

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500008

The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture

Hasenack,Beatriz S.; Botelho,Maria Valéria J.; Lauretti,Flávio; Melo,Fernando L. de; Orlandi,Janaina M.; Linhares,Rosa Elisa C.; Ueda,Marli; Nozawa,Carlos M..

Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.

Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus

Palavras-chave: Rotavirus; Concanavalin A; Cell culture.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200003

Barry,Aline Fernandes; Alfieri,Alice Fernandes; Stipp,Danilo Tancler; Alfieri,Amauri Alcindo.

Neonatal diarrhea is an important cause of economic losses for cattle farmers. The main viral etiologies of enteric diseases are group A rotaviruses (GARV) and the bovine coronavirus (BCoV). Although both viruses infect calves of the same age, the occurrence of mixed infections is still under studied. The present study describes the co-infection of BCoV and GARV in stool samples. Forty-four diarrheic fecal samples from calves up to 60 days old that had previously tested positive for GARV by SS-PAGE were analyzed using semi-nested PCR for BCoV. A product with 251 bp of the BCoV nucleoprotein gene was amplified in 15.9% (7/44) of the samples, demonstrating that co-infection is not an unusual event. These results reinforce the need for testing for both GARV...

Incidence of group A rotavirus in urban and rural areas of the city of Londrina-Brazil, from 1995 to 1997

Palavras-chave: Calf; Diarrhea; BCoV; Rotavirus; SN-PCR; Co-infection.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700006

Meneghetti,Ângelo Cesar; Bolognini,Andrea Maria; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Santos,Norma; Nozawa,Carlos Mitihiko.

Rotaviruses are common pathogens and the causal agents of acute diarrhea among children and young animals. The involvement of rotavirus in human diarrheal disease among population of urban and rural areas of the city of Londrina, Parana was evaluated. Nine hundred and five fecal specimens from persons with diarrhea were studied, being 686 and 219 from urban and rural areas, respectively. Thirty-eight samples (4,2%) were positive for rotavirus by polyacrylamide gel electrophoresis of viral RNA and latex agglutination test of which 36 were from urban and two from rural areas. Out of the positive specimens, 17 strains were further characterized by RT-PCR typing assay, resulting in 16 strains of G1 genotype while one sample was found to be a mixture of G1 and...

Molecular epidemiology of the human group A rotavirus in the Paraná State, Brazil

Palavras-chave: Rotavirus; Diarrhea; PAGE; LA; PCR.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300006

Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo.

From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed...

Seroprevalence of antibodies against group A rotavirus in cattle from a pioneer frontier in Brazilian Amazon

Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008

Braz. J. Vet. Res. Anim. Sci.

Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio.

Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group.

The infectivity of pig rotavirus in stools

Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003

Braz. J. Vet. Res. Anim. Sci.

RAMOS,Ana Paula Dores; STEFANELLI,Carla Cristina; LINHARES,Rosa Elisa Carvalho; BRITO,Benito Guimarães de; NOZAWA,Carlos Mitihiko.

Rotaviruses are the major pathogen for both human and animal. They affect young animals in intensive rearing and cause great economic losses. This study intended to evaluate the infectivity of porcine rotavirus maintained for 32 months at approximately 10ºC. in the original stools specimens. Thirty stools specimens of 1-4-week-old piglets originating from breeding farms located in the southwest region of the Paraná State were selected for this study. They were randomly chosen from stools samples positive for rotavirus by polyacrylamide gel electrophoresis (PAGE) at the time of collection. The thirty stools samples maintained for 32 months at approximately 10ºC were re-tested by PAGE and 11 out of 30 were still positive showing the integrity of the eleven...