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Cryopreserved sperm for oocyte fertilization of dourado Salminus brasiliensis Ciência Rural
Weingartner,Marcos; Zanandrea,Ana Carolina Volpato; Zaniboni Filho,Evoy.
Salminus brasiliensis is a migratory fish that has attracted considerable interest for aquaculture. Several procedures for induced spawning are known; however, there is a lack of protocol which enables the use of cryopreserved semen. Therefore, this study was conducted to investigate the use of cryopreserved semen using different volumes of cryopreserved semen relative to oocytes, different activators solutions and different maintenance time during the fertilization of dourado to evaluate the impact of these parameters on the fertilization rate. The semen was collected, cryopreserved in 0.5mL straws and stored in a dry shipper. Oocytes samples were fertilized according to each treatment. The different activator solutions and the contact times of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Frozen semen; Gamete ratio; Semen activation; Activator solution.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500892
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