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Registros recuperados: 38
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Isolation of Salmonella enterica subsp. enterica (O:4,5:i) and Salmonella enterica subsp. Typhimurium from free-living domestic pigeons (Columba livia) Arq. Bras. Med. Vet. Zootec.
Rocha-e-Silva,R.C.; Cardoso,W.M.; Teixeira,R.S.C.; Albuquerque,Á.H.; Horn,R.V.; Lopes,E.S.; Gomes Filho,V.J.R.; Almeida,C.P.; Santos,I.C.L.; Machado,D.N.; Lima,S.V.G.; Carneiro,I.S..
The present study reports the isolation of Salmonellaenterica in organs of free-living domestic pigeons. In the clinic examination, the presence of feces in the peri-cloacal and abdominal regions were observed, as well as symptoms such as cachexy, incoordination and opisthotonos. Before any therapeutic protocol was applied the bird died and a necropsy was then performed for the removal of spleen, liver, kidney and intestine for bacteriological examination and antibiotic sensitivity test. Salmonella enterica subsp.enterica (O:4,5:i-) and Salmonellaenterica subsp. enterica serovar Typhimurium were isolated from the liver and intestine and the sensitivity test demonstrated that these strains are sensitive to several antibiotics.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolation; Necropsy; Bacteriological examination; Sensitivity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501435
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Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR BABT
Ma,Min Min; Jiang,Dan; Li,Shuang Ya; Wu,Yuan; Meng,Ju; Huang,Jiang Yao.
ABSTRACT Adulterant herbal materials are threats to import and export trade and consumer safety. In this study, we established a simple and rapid examination system for the detection of Phellodendron chinense Schneid. Two detection methods, real-time fluorescence quantitative PCR (real-time PCR) and loop-mediated isothermal amplification (LAMP), were developed for traditional Chinese medicine detection, and their specificity and sensitivity were compared. The DNA of P. chinense was extracted and its special periods amplified with designed primers. Real-time PCR and LAMP experiments were conducted to test the specificity of primers in contrast to other similar species. The template concentration was diluted from 101 ng/µL to 10-5 ng/µL in order to contrast...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Traditional Chinese medicine; Real-time PCR; Phellodendron chinense Schneid; LAMP; Specificity; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100314
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Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG + 874 A>T polymorphism BJID
Carneiro,Valdirene Leão; Bendicho,Maria Teresita; Santos,Rosalina Guedes; Casela,Marilda; Netto,Eduardo M.; Mota,Scarlet Torres Moraes; Pina,Iza Cristina Araújo; Nascimento,Roberto Meyer; Freire,Songeli Menezes; Barbosa,Theolis.
ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis infection; Genetic variation; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300202
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Evaluation of a 1-h 75-g oral glucose tolerance test in the diagnosis of gestational diabetes BJMBR
Campos,M.A.A.; Reichelt,A.A.J.; Façanha,C.; Forti,A.C.; Schmidt,M.I..
In order to evaluate the performance of a 1-h 75-g oral glucose tolerance test (OGTT) for the diagnosis of gestational diabetes mellitus (GDM), a cohort of 4998 women, 20 years or older, without previous diabetes being treated in prenatal care clinics in Brazil answered a questionnaire and performed a 75-g OGTT including fasting, 1-h and 2-h glucose measurements between their 24th and 28th gestational weeks. Pregnancy outcomes were transcribed from medical registries. GDM was defined according to WHO criteria (fasting: ≥126 mg/dL; 2-h value: ≥140 mg/dL) and macrosomia as a birth weight equal to or higher than 4000 g. Areas under the receiver operator characteristic curve (AUC) were compared and diagnostic properties of various cut-off points were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gestational diabetes; Diagnosis; Macrosomia; 1-h OGTT; Sensitivity; Specificity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800008
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Zika detection: comparison of methodologies BJM
Colombo,Tatiana Elias; Terzian,Ana Carolina Bernardes; Araújo Júnior,João Pessoa; Parreira,Ricardo; Cabrera,Eliana Márcia Sotello; Santos,Izalco Nuremberg Penha dos; Reis,Andréia Francesli Negri; Costa,Fabiana Rodrigues; Cruz,Lilian Elisa Arão Antônio; Rombola,Patrícia Lopes; Nogueira,Maurício Lacerda.
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zika virus; Detection; Sensitivity; Specificity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144
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Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD BJM
Leal,C.A.G.; Carvalho-Castro,G.A.; Cottorello,A.C.; Leite,R.C.; Figueiredo,H.C.P..
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clinical samples; Sensitivity; Real-time PCR; WSSV; WSD.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038
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Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite BJM
Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe.
Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p > 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p < 0.05). The mono and dual-species biofilms were subjected to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310
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Antifungal activity of lectins against yeast of vaginal secretion BJM
Gomes,Bruno Severo; Siqueira,Ana Beatriz Sotero; Maia,Rita de Cássia Carvalho; Giampaoli,Viviana; Teixeira,Edson Holanda; Arruda,Francisco Vassiliepe Sousa; Nascimento,Kyria Santiago do; Lima,Adriana Nunes de; Souza-Motta,Cristina Maria; Cavada,Benildo Sousa; Porto,Ana Lúcia Figueiredo.
Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Sensitivity; Lectins.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200042
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Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan BJM
Khan,Anwar Sheed; Ali,Sajid; Khan,Muhammad Tahir; Ahmed,Sajjad; Khattak,Yasir; Abduljabbar,; Irfan,Muhammad; Sajjad,Wasim.
ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GeneXpert; LED-FM microscopy; Sensitivity; Specificity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400909
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Chemical management in fungicide sensivity of Mycosphaerella fijiensis collected from banana fields in México BJM
Aguilar-Barragan,Alejandra; García-Torres,Ana Elisa; Odriozola-Casas,Olga; Macedo-Raygoza,Gloria; Ogura,Tetsuya; Manzo-Sánchez,Gilberto; James,Andrew C.; Islas-Flores,Ignacio; Beltrán-García,Miguel J..
The chemical management of the black leaf streak disease in banana caused by Mycosphaerella fijiensis (Morelet) requires numerous applications of fungicides per year. However this has led to fungicide resistance in the field. The present study evaluated the activities of six fungicides against the mycelial growth by determination of EC50 values of strains collected from fields with different fungicide management programs: Rustic management (RM) without applications and Intensive management (IM) more than 25 fungicide application/year. Results showed a decreased sensitivity to all fungicides in isolates collected from IM. Means of EC50 values in mg L-1 for RM and IM were: 13.25 ± 18.24 and 51.58 ± 46.14 for azoxystrobin, 81.40 ± 56.50 and 1.8575 ± 2.11 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Banana; Fungicides; Mycosphaerella fijiensis; Sensitivity; Sigatoka.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100051
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Variation of water potential and trunk diameter answer as sensitivity to the water availability in table grapes Chilean J. Agric. Res.
Silva-Contreras,Claudio; Sellés-Von Schouwen,Gabriel; Ferreyra-Espada,Raul; Silva-Robledo,Herman.
Variation in trunk diameter (TDV) has been proposed as an indicator of the water status of grape vines, with apparently contradictory results. In Vitis vinifera L. var. Crimson Seedless we evaluated the water potential (&#936;), an indicator normally used to determine the water status of grapes, and TDV, comparing two irrigation treatments which restored 100% or 50% of the ETc, to compare the sensitivity of these two indicators to a decrease in soil water. Two evaluation periods of 23 d each were used, the first during the exponential growth phase and the second in the post-veraison period, when the trunk stops growing. In both periods TDV showed coefficients of variation greater than 25%, compared to the 7-10% recorded for water potential. However,...
Tipo: Journal article Palavras-chave: Water potential; Variable displacement sensor; Sensitivity; Itis vinifera.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000400001
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Evaluation of the performance of different plastics used to seal nylon cDNA arrays Ciência e Agrotecnologia
Costa Netto,Antônio Paulino da; Drummond,Rodrigo Duarte; Felix,Juliana de Maria; Jorge,Renato Atílio; Menossi,Marcelo.
cDNA arrays are a powerful tool for discovering gene expression patterns. Nylon arrays have the advantage that they can be re-used several times. A key issue in high throughput gene expression analysis is sensitivity. In the case of nylon arrays, signal detection can be affected by the plastic bags used to keep membranes humid. In this study, we evaluated the effect of five types of plastics on the radioactive transmittance, number of genes with a signal above the background, and data variability. A polyethylene plastic bag 69 μm thick had a strong shielding effect that blocked 68.7% of the radioactive signal. The shielding effect on transmittance decreased the number of detected genes and increased the data variability. Other plastics which were thinner...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Signal detection; Nylon arrays; Sensitivity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700031
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Uso de los modelos credit scoring en microfinanzas. Colegio de Postgraduados
Escalona Cortés, Arturo.
En el presente trabajo se presenta un modelo credit scoring en microfinanzas el cual permita calificar los solicitantes de un microcrédito. El modelo propuesto se basa en el modelo de regresión logística. La evaluación del modelo incluye el ajuste medido por la estadística Hosmer-Lemeshow, poder predictivo medido por la R2, poder discriminatorio medido por la curva ROC y área bajo la curva ROC. Además, se determinó un pundo de corte para validar el modelo. La información de clientes durante el período de mayo de 2008 a abril de 2009 de la empresa microfinanciera MásKapital se utilizó como base para desarrollar y calibrar el modelo. Se obtuvo un buen ajuste de este, reflejado en los resultados de validación que, presentan una clasificación total correcta...
Palavras-chave: Riesgo crediticio; Regresión logística; Microcréditos; Sensibilidad; Especificidad; Credit risk; Logistic regression; Microcredit; Sensitivity; Specificity; Maestría; Estadística.
Ano: 2011 URL: http://hdl.handle.net/10521/414
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Uso de los modelos credit scoring en microfinanzas. Colegio de Postgraduados
Escalona Cortés, Arturo.
En el presente trabajo se presenta un modelo credit scoring en microfinanzas el cual permita calificar los solicitantes de un microcrédito. El modelo propuesto se basa en el modelo de regresión logística. La evaluación del modelo incluye el ajuste medido por la estadística Hosmer-Lemeshow, poder predictivo medido por la R2, poder discriminatorio medido por la curva ROC y área bajo la curva ROC. Además, se determinó un pundo de corte para validar el modelo. La información de clientes durante el período de mayo de 2008 a abril de 2009 de la empresa microfinanciera MásKapital se utilizó como base para desarrollar y calibrar el modelo. Se obtuvo un buen ajuste de este, reflejado en los resultados de validación que, presentan una clasificación total correcta...
Palavras-chave: Riesgo crediticio; Regresión logística; Microcréditos; Sensibilidad; Especificidad; Credit risk; Logistic regression; Microcredit; Sensitivity; Specificity; Maestría; Estadística.
Ano: 2011 URL: http://hdl.handle.net/10521/414
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1-MCP efficiency in quality of ornamental peppers Horticultura Brasileira
Ribeiro,Wellington S; Carneiro,Clebson dos S; França,Christiane de Fátima M; Pinto,Cleide Maria F; Lima,Paula Cristina C; Finger,Fernando L.
ABSTRACT 1-MCP has been effectively used as an antagonist of ethylene deleterious actions in flowers and ornamental plants. The objective was to evaluate the efficiency of 1-MCP (0.0, 0.5, 1.0 and 1.5 g m-3) in maintaining post-production quality of exogenous ethylene sensitive ornamental pepper. Two commercial varieties were evaluated: Pimenta Colorida and Pimentão Ornamental, both Capsicum annuum. The pre-treatment with 1-MCP, regardless of the applied concentration, was effective in blocking the ethylene action reducing the leaves abscission, flowers and fruits of both genotypes by 100%. Plants pretreated with 1-MCP had postproduction life increased by eight and nine days for Pimenta Colorida and Pimentão Ornamental, respectively. The 1-MCP application...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capsicum annuum; Ornamental plants; Sensitivity; Transport.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400510
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Triggering mechanisms of slope instability processes and sediment failures on continental margins: a geotechnical approach ArchiMer
Sultan, Nabil; Cochonat, Pierre; Canals, M; Cattaneo, Antonio; Dennielou, Bernard; Haflidason, H; Laberg, J; Long, D; Mienert, J; Trincardi, F; Urgeles, R; Vorren, T; Wilson, C.
The Costa target areas exhibit the variability of slope instabilities needed to improve our understanding of sediment physical and mechanical properties in areas prone to sliding. That is why in this project, we have analysed the different slope failures events from different parts of the Costa target areas, which reflect diverse triggering mechanisms. The aim of the first part of this study was to identify the geotechnical response of the sediment to different external mechanisms (earthquake, rapid sedimentation and gas hydrate melting). We have focused on the relation between external mechanisms and the consequence change in the in-situ stress state and the physical, mechanical, and elastic properties of the sediment. In the second part of the paper, the...
Tipo: Text Palavras-chave: Slope instability; Sensitivity; Sedimentation; Hydrate; Earthquake.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-700.pdf
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Simulation de la dynamique de pêcherie : modélisation, complexité et incertitude ArchiMer
Mahevas, Stephanie.
Fisheries are defined by a set of fishermen and of marine populations impacting each other in a spatial-limited marine ecosystem. Within the over-exploitation context and according to ratified sustainable management objectives, my research has been focused on the development and a caution use of a mechanistic model describing fisheries dynamics. This model allows for integrating existing knowledge of fisheries. It aims at improving the understanding of fisheries functioning and at investigating the impact of fishing regulations on fisheries dynamics. This modelling exercise has supported many methodological developments for its parametrisation and its use in uncertainty context. Concerned with promoting a correct use of a complex model (that ambiguity link...
Tipo: Text Palavras-chave: Statistics; Spatial; Management; Optimal complexity; Sensitivity; ISIS Fish; Statistiques; Spatial; Gestion; Complexité optimale; Sensibilité; ISIS Fish.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/these-7302.pdf
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Sensitivity and variance estimators for virtual population analysis and the equilibrium yield per recruit model ArchiMer
Pelletier, Dominique.
Fish stock assessment makes Widespread use of Virtual Population Analysis (VPA) and long-term equilibrium prediction models. Although there have been some statistical investigations about the properties of VPA results, apparently there are no complete analyse about predictions statistical and numerical behaviour. However, management decisions generally rely upon production forecasts. This paper compares stability properties of VPA and the yield per recruit model with regard to various errors concerning all the input parameters. Robustness fis assessed by means of first-ordo sensitivities whereas variances are inferred from delta-method estimators. Sensitivity of yield per recruit appears to be mainly due to terminal mortality rate and age-specific weights,...
Tipo: Text Palavras-chave: Analyse de population virtuelle; Rendement par recrue; Sensibilité; Estimation de la variance; Méthode Delta; Virtual Population Analysis; Yield per recruit; Sensitivity; Variance estimation; Delta-method.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00189/30068/28556.pdf
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Intégrité des habitats benthiques du plateau continental face aux perturbations physiques naturelles et aux arts trainants : quelles méthodes d'observations ? Comment suivre le retour vers un bon état écologique ? ArchiMer
Jac, Cyrielle.
In 2008, the European Union drew up the Marine Strategy Framework Directive (MSFD) with the aim of achieving Good Environmental Status of marine waters. By linking the changes observed to the pressures experienced, this directive is defined as a tool designed to better control environmental degradation factors and to assess their implications. Several descriptors are defined within the framework of MSFD, in particular descriptors 1 relating to the biological diversity of the seabed and 6 relating to the integrity of the seabed (i.e. the quality of its structures and functions). As dragging (trawling and dredging) is the main source of disturbance of continental shelf seabed, it is essential to study its impact. However, its effects depend on several...
Tipo: Text Palavras-chave: Mégafaune benthique; Méthode d'échantillonnage; Impact de la pêche; Sensibilité; Facteurs environnementaux; Bon état écologique; Benthic megafauna; Sampling methods; Fishing impact; Sensitivity; Natural drivers; Good environmental status.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00679/79149/81655.pdf
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Validation of in situ hybridisation and histology assays for the detection of the oyster parasite Marteilia refringens ArchiMer
Thebault, Anne; Bergman, S; Pouillot, R; Le Roux, Frédérique; Berthe, Franck.
An in situ hybridisation technique has been developed for the detection of infection in oysters with Marteilia refringens with particular emphasis on light infections or confirmation of suspected cases by means of histology. Although validation of new diagnostic methods is usually achieved by comparison with standard techniques, in our case the sensitivity and specificity of the standard (histology) had not previously been established. Another point to consider is that surveillance and monitoring frequently target populations displaying different levels of prevalence under different field conditions. The objective of our study was to evaluate the sensitivity and specificity values of in situ hybridisation and histology for the detection of M refringens,...
Tipo: Text Palavras-chave: Ostrea edulis; Marteilia refringens; Latent models; Specificity; Sensitivity; Validation; Diagnostic.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3624.pdf
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