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Registros recuperados: 38 | |
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Rocha-e-Silva,R.C.; Cardoso,W.M.; Teixeira,R.S.C.; Albuquerque,Á.H.; Horn,R.V.; Lopes,E.S.; Gomes Filho,V.J.R.; Almeida,C.P.; Santos,I.C.L.; Machado,D.N.; Lima,S.V.G.; Carneiro,I.S.. |
The present study reports the isolation of Salmonellaenterica in organs of free-living domestic pigeons. In the clinic examination, the presence of feces in the peri-cloacal and abdominal regions were observed, as well as symptoms such as cachexy, incoordination and opisthotonos. Before any therapeutic protocol was applied the bird died and a necropsy was then performed for the removal of spleen, liver, kidney and intestine for bacteriological examination and antibiotic sensitivity test. Salmonella enterica subsp.enterica (O:4,5:i-) and Salmonellaenterica subsp. enterica serovar Typhimurium were isolated from the liver and intestine and the sensitivity test demonstrated that these strains are sensitive to several antibiotics. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Necropsy; Bacteriological examination; Sensitivity. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501435 |
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Ma,Min Min; Jiang,Dan; Li,Shuang Ya; Wu,Yuan; Meng,Ju; Huang,Jiang Yao. |
ABSTRACT Adulterant herbal materials are threats to import and export trade and consumer safety. In this study, we established a simple and rapid examination system for the detection of Phellodendron chinense Schneid. Two detection methods, real-time fluorescence quantitative PCR (real-time PCR) and loop-mediated isothermal amplification (LAMP), were developed for traditional Chinese medicine detection, and their specificity and sensitivity were compared. The DNA of P. chinense was extracted and its special periods amplified with designed primers. Real-time PCR and LAMP experiments were conducted to test the specificity of primers in contrast to other similar species. The template concentration was diluted from 101 ng/µL to 10-5 ng/µL in order to contrast... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Traditional Chinese medicine; Real-time PCR; Phellodendron chinense Schneid; LAMP; Specificity; Sensitivity. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100314 |
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Carneiro,Valdirene Leão; Bendicho,Maria Teresita; Santos,Rosalina Guedes; Casela,Marilda; Netto,Eduardo M.; Mota,Scarlet Torres Moraes; Pina,Iza Cristina Araújo; Nascimento,Roberto Meyer; Freire,Songeli Menezes; Barbosa,Theolis. |
ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis infection; Genetic variation; Sensitivity. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300202 |
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Colombo,Tatiana Elias; Terzian,Ana Carolina Bernardes; Araújo Júnior,João Pessoa; Parreira,Ricardo; Cabrera,Eliana Márcia Sotello; Santos,Izalco Nuremberg Penha dos; Reis,Andréia Francesli Negri; Costa,Fabiana Rodrigues; Cruz,Lilian Elisa Arão Antônio; Rombola,Patrícia Lopes; Nogueira,Maurício Lacerda. |
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison;... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zika virus; Detection; Sensitivity; Specificity. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144 |
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Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe. |
Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p > 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p < 0.05). The mono and dual-species biofilms were subjected to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310 |
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Gomes,Bruno Severo; Siqueira,Ana Beatriz Sotero; Maia,Rita de Cássia Carvalho; Giampaoli,Viviana; Teixeira,Edson Holanda; Arruda,Francisco Vassiliepe Sousa; Nascimento,Kyria Santiago do; Lima,Adriana Nunes de; Souza-Motta,Cristina Maria; Cavada,Benildo Sousa; Porto,Ana Lúcia Figueiredo. |
Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Yeast; Sensitivity; Lectins. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200042 |
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Khan,Anwar Sheed; Ali,Sajid; Khan,Muhammad Tahir; Ahmed,Sajjad; Khattak,Yasir; Abduljabbar,; Irfan,Muhammad; Sajjad,Wasim. |
ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GeneXpert; LED-FM microscopy; Sensitivity; Specificity. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400909 |
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Aguilar-Barragan,Alejandra; García-Torres,Ana Elisa; Odriozola-Casas,Olga; Macedo-Raygoza,Gloria; Ogura,Tetsuya; Manzo-Sánchez,Gilberto; James,Andrew C.; Islas-Flores,Ignacio; Beltrán-García,Miguel J.. |
The chemical management of the black leaf streak disease in banana caused by Mycosphaerella fijiensis (Morelet) requires numerous applications of fungicides per year. However this has led to fungicide resistance in the field. The present study evaluated the activities of six fungicides against the mycelial growth by determination of EC50 values of strains collected from fields with different fungicide management programs: Rustic management (RM) without applications and Intensive management (IM) more than 25 fungicide application/year. Results showed a decreased sensitivity to all fungicides in isolates collected from IM. Means of EC50 values in mg L-1 for RM and IM were: 13.25 ± 18.24 and 51.58 ± 46.14 for azoxystrobin, 81.40 ± 56.50 and 1.8575 ± 2.11 for... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Banana; Fungicides; Mycosphaerella fijiensis; Sensitivity; Sigatoka. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100051 |
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Silva-Contreras,Claudio; Sellés-Von Schouwen,Gabriel; Ferreyra-Espada,Raul; Silva-Robledo,Herman. |
Variation in trunk diameter (TDV) has been proposed as an indicator of the water status of grape vines, with apparently contradictory results. In Vitis vinifera L. var. Crimson Seedless we evaluated the water potential (Ψ), an indicator normally used to determine the water status of grapes, and TDV, comparing two irrigation treatments which restored 100% or 50% of the ETc, to compare the sensitivity of these two indicators to a decrease in soil water. Two evaluation periods of 23 d each were used, the first during the exponential growth phase and the second in the post-veraison period, when the trunk stops growing. In both periods TDV showed coefficients of variation greater than 25%, compared to the 7-10% recorded for water potential. However,... |
Tipo: Journal article |
Palavras-chave: Water potential; Variable displacement sensor; Sensitivity; Itis vinifera. |
Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000400001 |
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Ribeiro,Wellington S; Carneiro,Clebson dos S; França,Christiane de Fátima M; Pinto,Cleide Maria F; Lima,Paula Cristina C; Finger,Fernando L. |
ABSTRACT 1-MCP has been effectively used as an antagonist of ethylene deleterious actions in flowers and ornamental plants. The objective was to evaluate the efficiency of 1-MCP (0.0, 0.5, 1.0 and 1.5 g m-3) in maintaining post-production quality of exogenous ethylene sensitive ornamental pepper. Two commercial varieties were evaluated: Pimenta Colorida and Pimentão Ornamental, both Capsicum annuum. The pre-treatment with 1-MCP, regardless of the applied concentration, was effective in blocking the ethylene action reducing the leaves abscission, flowers and fruits of both genotypes by 100%. Plants pretreated with 1-MCP had postproduction life increased by eight and nine days for Pimenta Colorida and Pimentão Ornamental, respectively. The 1-MCP application... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum annuum; Ornamental plants; Sensitivity; Transport. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400510 |
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Sultan, Nabil; Cochonat, Pierre; Canals, M; Cattaneo, Antonio; Dennielou, Bernard; Haflidason, H; Laberg, J; Long, D; Mienert, J; Trincardi, F; Urgeles, R; Vorren, T; Wilson, C. |
The Costa target areas exhibit the variability of slope instabilities needed to improve our understanding of sediment physical and mechanical properties in areas prone to sliding. That is why in this project, we have analysed the different slope failures events from different parts of the Costa target areas, which reflect diverse triggering mechanisms. The aim of the first part of this study was to identify the geotechnical response of the sediment to different external mechanisms (earthquake, rapid sedimentation and gas hydrate melting). We have focused on the relation between external mechanisms and the consequence change in the in-situ stress state and the physical, mechanical, and elastic properties of the sediment. In the second part of the paper, the... |
Tipo: Text |
Palavras-chave: Slope instability; Sensitivity; Sedimentation; Hydrate; Earthquake. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-700.pdf |
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Mahevas, Stephanie. |
Fisheries are defined by a set of fishermen and of marine populations impacting each other in a spatial-limited marine ecosystem. Within the over-exploitation context and according to ratified sustainable management objectives, my research has been focused on the development and a caution use of a mechanistic model describing fisheries dynamics. This model allows for integrating existing knowledge of fisheries. It aims at improving the understanding of fisheries functioning and at investigating the impact of fishing regulations on fisheries dynamics. This modelling exercise has supported many methodological developments for its parametrisation and its use in uncertainty context. Concerned with promoting a correct use of a complex model (that ambiguity link... |
Tipo: Text |
Palavras-chave: Statistics; Spatial; Management; Optimal complexity; Sensitivity; ISIS Fish; Statistiques; Spatial; Gestion; Complexité optimale; Sensibilité; ISIS Fish. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/these-7302.pdf |
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Registros recuperados: 38 | |
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