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	Provedor de dados BABT (15) BJM (12) Electron. J. Biotechnol. (3) AgEcon (1) BJMBR (1) Rev. Bras. Ciênc. Avic. (1) Mais... Autor Soccol,Carlos Ricardo (5) Gomes,Eleni (2) Guimarães,Luis Henrique Souza (2) Jorge,João Atílio (2) Monti,Rubens (2) Pandey,Ashok (2) Szakacs,George (2) Vandenberghe,Luciana Porto de Souza (2) Adnan,Ahmad (1) Aguilera-Barreyro,Araceli (1) Ahmad,Waqar (1) Al-Dhabi,Naif Abdullah (1) Alegre,Ana Cláudia Paiva (1) Almeida,Mareci Mendes de (1) Amaro-Reyes,Aldo (1) Arabi,Mohammed Imad Eddin (1) Araújo,Lúcia de Fátima (1) Ayub,Ricardo Antônio (1) Badiale-Furlong,Eliana (1) Bakri,Yasser (1) Mais... Palavra-chave Solid-state fermentation (33) Aspergillus niger (4) Submerged fermentation (4) Wheat bran (3) Agro-industrial residues (2) Aspergillus (2) Aspergillus terreus (2) Cellulase (2) Citric acid (2) Lipase (2) Polygalacturonase (2) Protease (2) Xylanase (2) "shimeji" culture residue (1) 2S albumin (1) 6-pentyl-α-pyrone (1) Agro-residues (1) Alkaline xylanase (1) Alpha amylase (1) Amylase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (29) Journal article (3) Journal Article (1) Ano 2019 (1) 2016 (2) 2015 (2) 2014 (1) 2012 (4) 2011 (5) 2010 (1) 2009 (2) 2008 (3) 2007 (1) 2006 (3) 2005 (6) 2004 (1) 2001 (1) Mais... País Brazil (29) Chile (3) United States (1) Idioma Inglês (33)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 33 Primeira ... 1 2 ... Última Characterization of a multi-tolerant tannin acyl hydrolase II from Aspergillus carbonarius produced under solid-state fermentation Electron. J. Biotechnol. Valera,Larissa Serrani; Jorge,João Atílio; Guimarães,Luis Henrique Souza. Background Tannases are enzymes with biotechnological potential produced mainly by microorganisms as filamentous fungi. In this context, the production and characterization of a multi-tolerant tannase from Aspergillus carbonarius is described. Results The filamentous fungus A. carbonarius produced high levels of tannase when cultivated under solid-state fermentation using green tea leaves as substrate/carbon source and tap water at a 1:1 ratio as the moisture agent for 72 h at 30°C. Two tannase activity peaks were obtained during the purification step using DEAE-Cellulose. The second peak (peak II) was purified 11-fold with 14% recovery from a Sepharose CL-6B chromatographic column. The tannase from peak II (tannase II) was characterized as a heterodimeric... Tipo: Journal article Palavras-chave: Aspergillus; Microbial enzymes; Solid-state fermentation; Tannase; Tannic acid. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600013 Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility Electron. J. Biotechnol. Regalado,Carlos; Vázquez-Obregón,Immer; García-Almendárez,Blanca Estela; Domínguez-Domínguez,Jorge; Aguilera-Barreyro,Araceli; Amaro-Reyes,Aldo. Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 2(9-5), and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a Draper-Lin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein)-1 was achieved at 48 hrs of SSF, and was validated by confirmatory... Tipo: Journal article Palavras-chave: Aspergillus niger GS1; Corn stover; Digestibility; Solid-state fermentation; Xylanolytic activity. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200009 Bioconversion of agro-industrial wastes for the production of fibrinolytic enzyme from Bacillus halodurans IND18: Purification and biochemical characterization Electron. J. Biotechnol. Vijayaraghavan,Ponnuswamy; Prakash Vincenta,Samuel Gnana; Valan Arasu,Mariadhas; Al-Dhabi,Naif Abdullah. Background: Agro-wastes were used for the production of fibrinolytic enzyme in solid-state fermentation. The process parameters were optimized to enhance the production of fibrinolytic enzyme from Bacillus halodurans IND18 by statistical approach. The fibrinolytic enzyme was purified, and the properties were studied. Results: A two-level full factorial design was used to screen the significant factors. The factors such as moisture, pH, and peptone were significantly affected enzyme production and these three factors were selected for further optimization using central composite design. The optimum medium for fibrinolytic enzyme production was wheat bran medium containing 1% peptone and 80% moisture with pH 8.32. Under these optimized conditions, the... Tipo: Journal article Palavras-chave: Agro-residues; Solid-state fermentation; Statistical approach; Thrombolytic agent. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200001 APPLICATION OF SOLID-STATE FERMENTATION FOR CELLULASE ENZYME PRODUCTION USING TRICHODERMA VIRIDE AgEcon Mojsov, Kiro. The Solid-state fermentation (SSF) is alternative to submerged fermentation for production antibiotics, single cell protein, enzymes, organic acids, biofuel, etc. However, the advantages of SSF in various processes are found to be greater than in submerged fermentation. This technique not only decreases the cost of the process but also makes product cheaper for consumers. The paper describes experimental application of SSF on wheat straw for production of mycelia protein and cellulase enzymes by Trichoderma viride. This actual waste from agriculture industry was used as a nourishing base by Trichoderma viride in SSF for cellulase enzyme production. Growth and enzymes production by Trichoderma viride were evaluated on wheat straw and alkali treatment... Tipo: Journal Article Palavras-chave: Solid-state fermentation; Trichoderma viride; Cellulase enzyme; Wheat straw agro-waste; Single cell protein.; Research and Development/Tech Change/Emerging Technologies; L65. Ano: 2010 URL: http://purl.umn.edu/92392 Immunomodulation Properties of Solid-State Fermented Laetiporussulphureus Ethanol Extracts in Chicken Peripheral Blood Monocytes In Vitro Rev. Bras. Ciênc. Avic. Lin,WC; Lee,MT; Lin,LJ; Chang,SC; Lee,TT. ABSTRACT Laetiporus sp. is recognized as a fungal species traditionally used for medicinal purposes. This study investigated the in-vitro effects of solid-state fermented Laetiporussulphureus ethanol extracts (LSE) for their immunomodulatory potential. Bioactive levels detected in the LSE on different days throughout the fermentation period revealed that the 12th day was the most efficient, with 7.19 ± 0.66 GAE/g DM crude phenolic content, 2.71 ± 0.03 UAE/g DM crude triterpenoid content, 12.93 ± 0.88 GCE/g DM crude polysaccharides, and 96.44 ± 0.2 mg/g DM ergosterol content. In-vitroLSE tests on chPBMC showed no cytotoxicity within a range of 0.05-1 mg/mL, but LPS-inhibited cell viability was improved, as well as LPS-induced nitric oxide (NO) production... Tipo: Info:eu-repo/semantics/article Palavras-chave: Laetiporussulphureus; In vitro; Immunomodulatory; Solid-state fermentation. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400316 Production and characterization of endo-polygalacturonase from Aspergillus niger in solid-state fermentation in double-surface bioreactor BABT Hendges,Diogo Henrique; Montanari,Queli; Malvessi,Eloane; Silveira,Mauricio Moura da. Endo-polygalacturonase (endo-PG) production by Aspergillus niger T0005/007-2 in solid medium with 170 mm of height was evaluated in a cylindrical double surface bioreactor in 96-h experiments. Cell concentration close to 92 mg.g -¹ dm (mg per g of dry medium) in the standard condition (static) was achieved, whereas in tests under forced aeration of 1.4 and 2.8 L.min-1. Kg-1 mm (L of air per minute per Kg of moist medium) and with the central shaft fungal biomass attained approximately 100 mg.g-1 dm. Superior endo-PG activity was obtained with the central-shaft system, 78 U.g-1 dm (units per g of dry medium). Forced aeration and pressure pulse showed no positive effect on the production of endo-PG, 45 U.g-1 dm and 28 U.g-1 dm, respectively. None of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Double-surface bioreactor; Endo-polygalacturonase; Solid-state fermentation; Enzyme stability. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200005 Relation between citric acid production by solid-state fermentation from cassava bagasse and respiration of Aspergillus niger LPB 21 in semi-pilot scale BABT Prado,Flávera Camargo; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo. The aim of this work was to study the relation between citric acid production and respiration of Aspergillus niger LPB 21 in solid-state fermentation of cassava bagasse. The experiments were carried out in horizontal drum bioreactor coupled with a gas chromatography system. Fermentation was conduced for 144 h with initial substrate moisture of 60% using heat-treated cassava bagasse as sole carbon source. The exhausted air from the bioreactor was analyzed for the monitoring of CO2 produced and O2 consumed in order to estimate the biomass biosynthesis by the fungal culture. The metabolic activity of A. niger growth was associated to citric acid production. The system using FERSOL software determined 4.372 g of biomass/g of consumed O2. Estimated and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citric acid; Solid-state fermentation; Cassava bagasse; Horizontal drum bioreactor; Fungus respiration. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400004 Microbial rennet produced by Mucor miehei in solid-state and submerged fermentation BABT Silveira,Guilherme Garcia da; Oliveira,Gustavo Monteiro de; Ribeiro,Eloizio Julio; Monti,Rubens; Contiero,Jonas. The aim of this work was to study the effect of carbon and nitrogen sources in submerged fermentation, and the casein effect on solid-state fermentation on rennin production by Mucor miehei. Biomass peaks reached 6.7; 8.1 and 8 g/L and enzymatic production peaks of 1,066; 857 and 480 Soxhlet Units (S.U.) for glucose concentration of 18; 25 and 35 g/L respectively. Flasks with baffles showed biomass peaks of 6.7; 8.3 and 10 g/L and enzyme activity peaks of 648; 279 and 300 S.U. for the same glucose concentration. The values of 923 and 667 S.U. were obtained when corn steep liquor and Proflo (Cottonseed Nutrients from Traders ®) were used. In SSF system the enzymatic activities were 414, 264 and 167 S.U., when using HCl 0.2 N, 0.3 N and 0.4 N respectively.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mucor miehei; Rennin; Clotting activity; Casein; Solid-state fermentation; Submerged fermentation. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800009 Lipase production by solid-state fermentation in fixed-bed bioreactors BABT Cavalcanti,Elisa d'Avila Costa; Gutarra,Melissa Limoeiro Estrada; Freire,Denise Maria Guimarães; Castilho,Leda dos Reis; Sant'Anna Júnior,Geraldo Lippel. In the present work, packed bed bioreactors were employed with the aim of increasing productivity and scaling up of lipase production using Penicillium simplicissimum in solid-state fermentation. The influence of temperature and air flow rate on enzyme production was evaluated employing statistical experimental design, and an empirical model was adjusted to the experimental data. It was shown that higher lipase activities could be achieved at lower temperatures and higher air flow rates. The maximum lipase activity (26.4 U/g) was obtained at the temperature of 27°C and air flow rate of 0.8 L/min. Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-state fermentation; Fixed-bed bioreactors; Lipase; Penicillium simplicissimum. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400010 Alpha amylase from a fungal culture grown on oil cakes and its properties BABT Ramachandran,Sumitra; Patel,Anil K.; Nampoothiri,Kesavan Madhavan; Chandran,Sandhya; Szakacs,George; Soccol,Carlos Ricardo; Pandey,Ashok. Solid-state fermentation was carried out for the production of alpha-amylase using Aspergillus oryzae. Different oil cakes such as coconut oil cake (COC) sesame oil cake (SOC), groundnut oil cake (GOC), palm kernel cake (PKC) and olive oil cake (OOC) were screened to be used as substrate for the enzyme production and also compared with wheat bran (WB). GOC was found to be the best producer of the enzyme among these. Combination of WB and GOC (1:1) resulted higher enzyme titres than the individual substrates. Maximum amount of enzyme (9196 U/gds) was obtained when SSF was carried out using WB + GOC, having initial moisture of 64% and supplemented with lactose and ammonium nitrate (1% each) at 30ºC for 72h using 2 mL spore suspension (6x10(7)spores/ml).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha amylase; Oil cakes; Solid-state fermentation; Partial purification; Aspergillus oryzae. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200019 Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation BABT Goulart,Antonio José; Carmona,Eleonora Cano; Monti,Rubens. Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45º C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 µmol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL) than at pH 9.0. Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline xylanase; Rhizopus stolonifer; Solid-state fermentation. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300001 Co-Production of Nattokinase and Poly (γ-Glutamic Acid) Under Solid-State Fermentation Using Soybean and Rice Husk BABT Nie,Guangjun; Zhu,Zhubin; Liu,Fang; Nie,Zhijie; Ye,Yuchao; Yue,Wenjin. ABSTRACTThe aim of this work was to study the co-production of nattokinase and poly (γ-glutamic acid) by Bacillus subtilis natto with soybean and rice husk under solid-state fermentation (SSF). The results showed that the size of soybean particle and rice husk significantly improved the co-production of nattokinase and poly (γ-glutamic acid), yielding 2503.4 IU/gs and 320 mg/gs, respectively in the improved culture medium composed of 16.7% soybean flour and 13.3% rice husk with 70% water content. The yields increased by approximate 7- and 2-fold factor relative to their original ones. Thus, the co-production of nattokinase and poly (γ-glutamic acid) under SSF could be considered as an efficient method to exploit agro-residues for economical production of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-production; Nattokinase; Poly (γ-glutamic acid); Rice husk; Solid-state fermentation. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500718 Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation BABT Costa,Jorge Alberto Vieira; Colla,Eliane; Magagnin,Glênio; Santos,Lucielen Oliveria dos; Vendruscolo,Mauricio; Bertolin,Telma Elita. Amyloglucosidase (AMG) and exo-polygalacturonase (exo-PG) were simultaneously produced by two different strains of Aspergillus niger in solid-state fermentation (SSF) using defatted rice-bran as substrate. The effect of Aspergillus niger strain (t0005/007-2 and/or CCT 3312), inoculum type (spore suspension or fermented bran) and addition of inducers (pectin and/or starch) to the culture media was studied using a 3² x 2¹ factorial experimental design. The production of AMG and exo-PG was significantly affected by fungal strain and inoculum type but inducers had no effect. The maximum yields obtained were 1310 U/g dm for AMG using a spore suspension of A. niger CCT 3312 and 50.2 U/g dm for exo-PG production, using A. niger t0005/007-2 and fermented bran as... Tipo: Info:eu-repo/semantics/article Palavras-chave: Amyloglucosidase; Exo-polygalacturonase; Rice-bran; Solid-state fermentation. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500003 Sperathe effects of solid-state fermentation in the functional properties of defatted rice bran and wheat bran BABT Silveira,Cristina Moreira da; Badiale-Furlong,Eliana. Functional properties of fermented bran produced by Aspergillus oryzae and Rhizopus sp. in a solid-state fermentation system were determined, with an aim to evaluate their application in food formulation. The defatted rice bran and wheat bran were inoculated with the spores of the cultures and incubated at 30º C for 72 h. Samples were withdrawn at 0, 24, 48 and 72 h. Protein content, protein solubility, in-vitro digestibility, gelation and water holding capacity were determined in bran with or without fermentation. Rhizopus sp. increased significantly the protein content (69.0 and 56.0%, respectively, for defatted rice bran and wheat bran); protein solubility (28.5 and 36.2) and water holding capacity (11.4% for wheat bran). When A. oryzae was used all... Tipo: Info:eu-repo/semantics/article Palavras-chave: Functional properties; Solid-state fermentation; Defatted rice bran; Wheat bran. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600027 Partial characterization of an inulinase produced by Aspergillus japonicus URM5633 BABT Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Porto,Tatiana Souza; Spier,Michele Rigon; Soccol,Carlos Ricardo; Porto,Ana Lúcia Figueiredo; Souza-Motta,Cristina Maria; Moreira,Keila Aparecida. Enzymes obtained by fermentation processes offer a number of advantages and have been widely researched and used throughout the world. This study aimed to partially characterise an inulinase produced from palm and cassava peel. The enzyme was produced via the solid-state fermentation of Aspergillus japonicus URM5633. The optimal temperatures were 50ºC and 55ºC, and the optimal pH values were 5.2 and 3.4 for inulinase fermentatively produced from palm and cassava peel, respectively. The thermostability measurements for inulinase produced in palm showed that the relative activity remained below 100% until 30 minutes of stability for all temperatures, but reached 106.8% at a temperature of 50ºC after 60 minutes. Inulinase from the crude extract of cassava... Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Characterisation; Solid-state fermentation; Aspergillus japonicus. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500005 Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation BABT Slivinski,Christiane Trevisan; Machado,Alex Vinicius Lopes; Iulek,Jorge; Ayub,Ricardo Antônio; Almeida,Mareci Mendes de. In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucoamylase; Aspergillus niger; Solid-state fermentation; Biochemical characterization; Purification. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018 Peptidase with Keratinolytic Activity Secreted by Aspergillus terreus During Solid-State Fermentation BABT Siqueira,Ana Claudia Rodrigues de; Rosa,Nathalia Gonsales da; Motta,Cristina Maria Souza; Cabral,Hamilton. The aim of this study was to evaluate peptidase production by Aspergillus terreus in solid-state bioprocess and evaluate its parameters. The best conditions were 5.0 g of wheat bran as substrate, incubation temperature 30°C, inoculum 2.0x105spores/g and 75% saline volume, with production reaching 677 U/mL (5400 U/g culture medium) after 72 h of fermentation. Biochemical characterization of the crude enzymatic extract showed the optimum pH and temperature of 6.5 and 55°C, respectively. The stability at different temperatures and pH values showed that the extract could endure different pH. The evaluation of the ions influence and inhibitors proved that the enzyme required an ion for better activity, which was corroborated with the inhibition of EDTA and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Solid-state fermentation; Keratinase; Wheat bran; Aspergillus terreus. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400514 Protein enrichment of cactus pear (Opuntia ficus - indica Mill) using Saccharomyces cerevisiae in solid-state fermentation BABT Araújo,Lúcia de Fátima; Medeiros,Ariosvaldo Nunes; Perazzo Neto,Américo; Oliveira,Líbia de Sousa Conrado; Silva,Flávio Luiz Honorato da. The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protein enrichment; Solid-state fermentation; Cactus pear; Yeast. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400020 Spore production of Beauveria bassiana from agro-industrial residues BABT Santa,Herta Stutz Dalla; Santa,Osmar Roberto Dalla; Brand,Débora; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo. The purpose of this work was to produce Beauveria bassiana by Solid-State Fermentation using agro-industrial residues and optimizing the cultivation conditions. Refused potatoes, coffee husks and sugar-cane bagasse were tested. The blend of refused potatoes and sugar-cane bagasse (60-40%) with particle size in the range of 0.8-2 mm was used in the fermentation experiments. In Erlenmeyer flasks the best spore production was achieved with the following conditions: incubation temperature 26º C; initial pH 6.0; inoculum concentration 10(7) spores.g-1.dw and initial moisture 75%. In the column type reactor using forced aeration under the optimized conditions, the maximum production (1.07x10(10)spores.g-1.dw) was obtained at the 10th day of fermentation. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agro-industrial residues; Beauveria bassiana; Solid-state fermentation. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400007 Rice bran as a substrate for proteolytic enzyme production BABT Sumantha,Alagarsamy; Deepa,Paul; Sandhya,Chandran; Szakacs,George; Soccol,Carlos Ricardo; Pandey,Ashok. Rice bran was used as the substrate for screening nine strains of Rhizopus sp. for neutral protease production by solid-state fermentation. The best producer, Rhizopus microsporus NRRL 3671, was used for optimizing the process parameters for enzyme production. Fermentation carried out with 44.44 % initial moisture content at a temperature of 30 C for 72 h was found to be the optimum for enzyme secretion by the fermenting organism. While most of the carbon supplements favored enzyme production, addition of casein resulted in a marginal increase in protease yield. Fermentation was then carried out under optimized conditions to obtain the crude extract of the enzyme, which was partially purified by precipitation and dialysis. A 3-fold increase in the enzyme... Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-state fermentation; Rice bran; Rhizopus; Neutral protease; Agro-industrial residues. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600019 Registros recuperados: 33 Primeira ... 1 2 ... Última

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