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Evaluation of antimicrobial activity of the endophytic actinomycete R18(6) against multiresistant Gram-negative bacteria Anais da ABC (AABC)
Carvalho,Tiele; Van Der Sand,Sueli.
Endophytic actinomycetes are promising sources of antimicrobial substances. This study evaluates the activity of metabolites produced by the endophytic actinomycete R18(6) against Gram-negative bacteria multiresistant to antimicrobials. R18(6) isolate was grown in submerged cultures under different conditions: carbon source, temperature, pH and incubation time to optimize antimicrobials production. The actinomycete grown in base medium supplemented with 1% glucose, pH 6.5 and incubation at 30 ºC for 96 h with shaking at 100 rpm, exhibited the highest activity against the used Gram-negative bacteria. Minimum inhibitory concentration (MIC) of the crude extract produced by the microorganism varied between 1/32 and 1/256. It had bactericide or bacteriostatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic actinomycetes; Streptomyces; Multiresistant Gram-negative bacteria; Extract.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100155
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In vitro Antimicrobial Assay of Actinomycetes in Rice AgainstXanthomonas oryzae pv. oryzicola and as Potential Plant Growth Promoter BABT
Hata,Erneeza Mohd; Sijam,Kamaruzaman; Ahmad,Zainal Abidin Mior; Yusof,Mohd Termizi; Azman,Noor Aisyah.
ABSTRACT The aim of this work was to invitro assay the antimicrobial activity of actinomycetes in rice against Xanthomonas oryzae pv. oryzicola and as potential plant growth promoter. A total of 92 actinomycete strains were isolated from different rice plant components and field locations. Of these, only 21.74% showed antagonistic activity against the Xoc pathogen. Molecular identification via 16s rRNA amplification revealed that 60% of the active antagonistic strains belonged to the genus Streptomyces. Isolates that demonstrated the highest antagonistic activity were also able to produce hydrolytic enzymes and plant growth-promoting hormones. Combination of preliminary screening based on in vitro antagonistic, hydrolytic enzyme and plant growth hormone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonistic activity; Hydrolytic enzyme; Plant growth-promoting agent; Streptomyces.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600821
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Isolation of endophytic actinomycetes from roots and leaves of maize (Zea mays L.) BABT
Araújo,Janete M. de; Silva,Adilson C. da; Azevedo,João L..
Actinomycetes were isolated from surface sterilized leaves and roots of maize. A total of 53 isolates were obtained, 31 of them from leaves and 22 from roots. The genus Microbispora was the most frequently found followed by the genera Streptomyces and Streptosporangium. From the isolated actinomycetes, 43.4% showed antimicrobial activity against one or more tested bacteria and yeast.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytes; Maize; Actinomycetes; Microbispora; Streptomyces; Streptosporangium.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400016
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Biological control of phytopathogenic fungi by endophytic actinomycetes isolated from maize (Zea mays L.) BABT
Costa,Francisco Gheler; Zucchi,Tiago Domingues; Melo,Itamar Soares de.
This work aimed a survey on the biodiversity of maize endophytic actinomycete, and an evaluation of their potential to control the phytopathogenic fungi. From several regions of São Paulo state, 40 strains were isolated from the healthy maize plants. The identification of these strains, based on morphological properties and fatty acid methyl ester (FAME) profile showed that most of them belonged to the Streptomyces genus. These isolates were first screened for the growth inhibition of phytopathogenic fungi and results showed that all the isolate were able to inhibit the development of at least one tested pathogen. Two selected isolates were then evaluated for the control of P. aphanidermatum in cucumber (Cucumis sativa L.) under greenhouse conditions....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytes; Actinomycete; Streptomyces; Pythium aphanidermatum; Dumping-off.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600009
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Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues BABT
Alberton,Luiz Romulo; Vandenberghe,Luciana Porto de Souza; Assmann,Ricardo; Fendrich,Ricardo Cancio; Rodriguéz-León,José; Soccol,Carlos Ricardo.
The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; Xylanase; Submerged fermentation; Solid-state agro-industrial fermentation; Residues.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022
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Biodiversity of Antimicrobial-Producing Actinomycetes Strains Isolated from Dry Dipterocarp Forest Soil in Northeast Thailand BABT
Chanthasena,Panjamaphon; Nantapong,Nawarat.
The increasing use of antibiotics has led to the development of drug-resistant microorganisms. The emergence of resistant strains has compromised the treatment and control of infectious diseases. As a result, the search and development of a novel class of antibiotic drugs are required. Actinomycetes have been recognized as a richest source of secondary metabolites including antibiotics. The aim of this study was to investigate the diversity of antibiotic-producing soil Actinomycetes from dry dipterocarp forest in Northeast of Thailand. The soil from this area has been known for its poor in nutrients and highly acidic with pH values around 4.5. In such a harsh condition, soil-inhabiting Actinomycetes elevate their protective mechanisms by inducing the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soil bacteria; Antibiotics; Actinomycetes; Streptomyces.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100417
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Antibiotics produced by Streptomyces BJID
Procópio,Rudi Emerson de Lima; Silva,Ingrid Reis da; Martins,Mayra Kassawara; Azevedo,João Lúcio de; Araújo,Janete Magali de.
Streptomyces is a genus of Gram-positive bacteria that grows in various environments, and its shape resembles filamentous fungi. The morphological differentiation of Streptomyces involves the formation of a layer of hyphae that can differentiate into a chain of spores. The most interesting property of Streptomyces is the ability to produce bioactive secondary metabolites, such as antifungals, antivirals, antitumorals, anti-hypertensives, immunosuppressants, and especially antibiotics. The production of most antibiotics is species specific, and these secondary metabolites are important for Streptomyces species in order to compete with other microorganisms that come in contact, even within the same genre. Despite the success of the discovery of antibiotics,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; Antibiotics; Resistance; Infection.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500011
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Production and characterization of a biosurfactant produced by Streptomyces sp. DPUA 1559 isolated from lichens of the Amazon region BJMBR
Santos,A.P.P.; Silva,M.D.S.; Costa,E.V.L.; Rufino,R.D.; Santos,V.A.; Ramos,C.S.; Sarubbo,L.A.; Porto,A.L.F..
Surfactants are amphipathic compounds containing both hydrophilic and hydrophobic groups, capable to lower the surface or interfacial tension. Considering the advantages of the use of biosurfactants produced by microorganisms, the aim of this paper was to develop and characterize a biosurfactant produced by Streptomyces sp. DPUA1559 isolated from lichens of the Amazon region. The microorganism was cultured in a mineral medium containing 1% residual frying soybean oil as the carbon source. The kinetics of biosurfactant production was accompanied by reducing the surface tension of the culture medium from 60 to values around 27.14 mN/m, and by the emulsification index, which showed the efficiency of the biosurfactant as an emulsifier of hydrophobic compounds....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosurfactant; Surface tension; Emulsification; Streptomyces; Soybean frying oil.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200606
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Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea BJM
Sreevidya,M.; Gopalakrishnan,S.; Kudapa,H.; Varshney,R.K..
Abstract The main objective of the present study was to isolate and characterize actinomycetes for their plant growth-promotion in chickpea. A total of 89 actinomycetes were screened for their antagonism against fungal pathogens of chickpea by dual culture and metabolite production assays. Four most promising actinomycetes were evaluated for their physiological and plant growth-promotion properties under in vitro and in vivo conditions. All the isolates exhibited good growth at temperatures from 20 °C to 40 °C, pH range of 7–11 and NaCl concentrations up to 8%. These were also found highly tolerant to Bavistin, slightly tolerant to Thiram and Captan (except VAI-7 and VAI-40) but susceptible to Benlate and Ridomil at field application levels and were found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chickpea; Actinomycetes; PGP; Streptomyces; Biocontrol; Gene expression.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100085
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Genome sequence of Streptomyces mangrovisoli MUSC 149T isolated from intertidal sediments BJM
Ser,Hooi-Leng; Tan,Wen-Si; Ab Mutalib,Nurul-Syakima; Yin,Wai-Fong; Chan,Kok-Gan; Goh,Bey-Hing; Lee,Learn-Han.
ABSTRACT As the largest genus in Actinobacteria family, Streptomyces species have the ability to synthesize numerous compounds of diverse structures with bioactivities. Streptomyces mangrovisoli MUSC 149T was previously isolated as a novel streptomycete from mangrove forest in east coast of Peninsular Malaysia. The high quality draft genome of MUSC 149T comprises 9,165,825 bp with G + C content of 72.5%. Through bioinformatics analysis, 21 gene clusters identified in the genome were associated with the production of bioactive secondary metabolites. The presence of these biosynthetic gene clusters in MUSC 149T suggests the potential exploitation of the strain for production of medically important compounds.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genome sequence; Streptomyces; Mangrove; AntiSMASH; Bioinformatics analysis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100013
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Glucose(xylose) isomerase production by Streptomyces sp. CH7 grown on agricultural residues BJM
Chanitnun,Kankiya; Pinphanichakarn,Pairoh.
Streptomyces sp. CH7 was found to efficiently produce glucose(xylose) isomerase when grown on either xylan or agricultural residues. This strain produced a glucose(xylose) isomerase activity of roughly 1.8 U/mg of protein when it was grown in medium containing 1% xylose as a carbon source. Maximal enzymatic activities of about 5 and 3 U/mg were obtained when 1% xylan and 2.5% corn husks were used, respectively. The enzyme was purified from a mycelial extract to 16-fold purity with only two consecutive column chromatography steps using Macro-prep DEAE and Sephacryl-300, respectively. The approximate molecular weight of the purified enzyme is 170 kDa, and it has four identical subunits of 43.6 kDa as estimated by SDS-PAGE. Its Km values for glucose and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural residues; Glucose(xylose) isomerase; Production; Purification; Streptomyces.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300035
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Draft genome sequence of Streptomyces sp. strain F1, a potential source for glycoside hydrolases isolated from Brazilian soil BJM
Melo,Ricardo Rodrigues de; Persinoti,Gabriela Felix; Paixão,Douglas Antonio Alvaredo; Squina,Fábio Márcio; Ruller,Roberto; Sato,Helia Harumi.
ABSTRACT Here, we show the draft genome sequence of Streptomyces sp. F1, a strain isolated from soil with great potential for secretion of hydrolytic enzymes used to deconstruct cellulosic biomass. The draft genome assembly of Streptomyces sp. strain F1 has 69 contigs with a total genome size of 8,142,296 bp and G + C 72.65%. Preliminary genome analysis identified 175 proteins as Carbohydrate-Active Enzymes, being 85 glycoside hydrolases organized in 33 distinct families. This draft genome information provides new insights on the key genes encoding hydrolytic enzymes involved in biomass deconstruction employed by soil bacteria.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinobacteria; Glycoside hydrolases; Streptomyces; Draft genome sequence; Soil bacteria.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400612
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Production of actinomycin-D by the mutant of a new isolate of Streptomyces sindenensis BJM
Praveen,Vandana; Tripathi,C.K.M.; Bihari,Vinod; Srivastava,S.C..
An actinomycin-D producing strain was isolated from soil and characterized as Streptomyces sindenensis. The culture was subjected to UV irradiation and a mutant with 400% higher actinomycin-D production was isolated (400 mg/l-1 as compared to 80 mg/l-1 produced by the parent). Production medium was optimized and antibiotic yield with the mutant was enhanced to 850 mg/l-1 which is 963% higher as compared with the parent.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycin-D; Antibiotics; Streptomyces; Mutation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400017
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Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics BJM
Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Javoreková,Soňa.
ABSTRACT Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolates matched those described for members of the genus Streptomyces. DK-15 strain exhibited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; 16S rRNA; MALDI-TOF MS; Tetrangomycin; Factumycin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400816
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Carbon catabolite repression of retamycin production by Streptomyces olindensis ICB20 BJM
Inoue,Olavo Ossamu; Schmidell Netto,Willibaldo; Padilla,Gabriel; Facciotti,Maria Cândida Reginato.
Retamycin is an anthracyclinic antitumoral complex produced by Streptomyces olindensis ICB20. In this work the influence of different glucose concentrations in the feed medium on the production of retamycin was studied. Chemostat cultures employing glucose concentration varying between 10 g/L and 25 g/L showed that use of high glucose concentration resulted in catabolite repression of the biosynthesis of the antitumoral. The highest specific retamycin production rate, qRTM = 7.8 mg/g.h, was obtained when glucose concentration was 10 g/L. The lowest value of qRTM, 2.5 mg/g.h, was observed when glucose concentration was 20 and 25 g/L. The residual glucose concentration varied from 0 to 13 g/L, as the glucose concentration in the feed was increased from 10 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; Anthracyclines; Carbon catabolite repression.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100012
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Poly R decolorization and APPL production by Streptomyces violaceoruber and Streptomyces spiroverticillatus BJM
Abou-Dobara,M.I.; Omar,N.F..
Two mesophilic streptomycetes (S. violaceoruber and S. spiroverticillatus) were selected to study their Poly R-478 decolorization ability and lignocellulose solubilizing activity. Both strains were able to degrade Poly R-478 dye and ferulic acid during growth on a minimal salts medium. The Poly R-478 decolorizing activities of both strains were induced by adding different carbon sources to the culture media. S. violaceoruber could decolorize 63% of Poly R-478 after 24 h. Both strains could solubilize straw and produce acid-precipitable polymeric lignin (APPL) with different efficiency. From the major extracellular enzymes recovery of both strains on rice and wheat straw, we can predicate that the biodegradation process was partial indicating a possible...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; APPL; Poly-R decolorization; Ferulic acid degradation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400008
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Streptomyces lunalinharesii 235 prevents the formation of a sulfate-reducing bacterial biofilm BJM
Rosa,Juliana Pacheco da; Tibúrcio,Samyra Raquel Gonçalves; Marques,Joana Montezano; Seldin,Lucy; Coelho,Rosalie Reed Rodrigues.
ABSTRACT Streptomyces lunalinharesii strain 235 produces an antimicrobial substance that is active against sulfate reducing bacteria, the major bacterial group responsible for biofilm formation and biocorrosion in petroleum reservoirs. The use of this antimicrobial substance for sulfate reducing bacteria control is therefore a promising alternative to chemical biocides. In this study the antimicrobial substance did not interfere with the biofilm stability, but the sulfate reducing bacteria biofilm formation was six-fold smaller in carbon steel coupons treated with the antimicrobial substance when compared to the untreated control. A reduction in the most probable number counts of planktonic cells of sulfate reducing bacteria was observed after treatments...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; Antimicrobial substance; Biofilm; Biocorrosion; Sulfate reducing bacteria.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300603
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Utilization of Agro-industrial Wastes for the Simultaneous Production of Amylase and Xylanase by Thermophilic Actinomycetes BJM
Singh,Renu; Kapoor,Vishal; Kumar,Vijay.
Agro-industrial wastes such as sugarcane bagasse, wheat bran, rice bran, corn cob and wheat straw are cheapest and abundantly available natural carbon sources. The present study was aimed to production of amylase and xylanase simultaneously using agro-industrial waste as the sole carbon source. Seven thermophilic strains of actinomycete were isolated from the mushroom compost. Among of these, strain designated MSC702 having high potential to utilize agro-industrial wastes for the production of amylase and xylanase. Strain MSC702 was identified as novel species of Streptomyces through morphological characterization and 16S rRNA gene sequence. Enzyme production was determined using 1% (w/v) of various agro-industrial waste in production medium containing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agro-industrial wastes; Thermophilic actinomycetes; Streptomyces; Amylase; Xylanase.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400039
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Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa BJPS
Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Císarová,Miroslava; Javoreková,Soňa.
In the present study we reported the antimicrobial activity of actinomycetes isolated from aridic soil sample collected in Karoo, South Africa. Eighty-six actinomycete strains were isolated and purified, out of them thirty-four morphologically different strains were tested for antimicrobial activity. Among 35 isolates, 10 (28.57%) showed both antibacterial and antifungal activity. The ethyl acetate extract of strain KRG-1 showed the strongest antimicrobial activity and therefore was selected for further investigation. The almost complete nucleotide sequence of the 16S rRNA gene as well as distinctive matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry (MALDI-TOF/MS) profile of whole-cell proteins acquired for strain KRG-1 led to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycin/antimicrobial activity; Streptomyces; Soil; 16S rRNA; MALDI-TOF MS.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100594
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電気分解により生じた塩素水によるジャガイモそうか病菌の殺菌について OAK
山本, 紳朗; 佃, 祥江; 前田, 征之; 金子, 知央; 安井, 篤司; 美濃, 羊輔; YAMAMOTO, Shinro; TSUKUDA, Yoshie; MAEDA, Masayuki; KANEKO, Tomohisa; YASUI, Atsushi; MINO, Yosuke.
http://www.obihiro.ac.jp/~library/kenkyu.html
Palavras-chave: 塩素; そうか病; 殺菌; Streptomyces; 電気分解; バレイショデンプン廃液.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3797
Registros recuperados: 24
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