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	Provedor de dados 58 (5) 52 (2) Autor Badino,Alberto Colli (3) Hokka,Carlos Osamu (2) Lima Filho,José Luiz de (2) Antonio,Tatiana (1) Araujo,Maria Lucia Gonsales da Costa (1) Baptista-Neto,Álvaro (1) Bellão,Carolina (1) Campos-Takaki,Galba Maria de (1) Carneiro-da-Cunha,Maria das Graças (1) Cassiano Filho,Luiz Claudio Macedo (1) Cavalcanti,Maria Taciana Holanda (1) Cavallieri,André Pastrelo (1) Cerri,Marcel Otavio (1) Corrêa,Tatiana (1) Cruz-Hernández,Isara Lourdes (1) Duarte,Helena Simões (1) Goto,Leandro Seiji (1) Lima,Vanderlei Aparecido de (1) Melo,Eduardo Henrique Magalhães de (1) Moreira,Keila Aparecida (1) Mais... Palavra-chave Streptomyces clavuligerus (7) Clavulanic acid (4) Alkaline protease (1) Amino acids supplementation (1) Complex medium (1) Continuous culture (1) Enzymatic potential (1) Extracellular proteases (1) Ferrous sulfate (1) Lipase (1) Lysine metabolism (1) Mutation (1) PH (1) Protease optimization (1) Protoplast (1) Salt-tolerant actinomycetes (1) Thermal stability (1) Β-lactam antibiotic (1) Mais... Tipo do documento Info:eu-repo/semantics/article (7) Ano 2018 (1) 2013 (1) 2012 (1) 2007 (1) 2005 (1) 2002 (1) 2001 (1) Mais... País Brazil (7) Idioma Inglês (7)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 7 Primeira ... 1 ... Última Comparisons between continuous and batch processing to produce clavulanic acid by Streptomyces clavuligerus 52 Baptista-Neto,Álvaro; Teodoro,Juliana Conceição; Cassiano Filho,Luiz Claudio Macedo; Badino,Alberto Colli; Hokka,Carlos Osamu. The aim of the present work was to compare CA production in continuous culture with and without cell recycling and in batch process by Streptomyces clavuligerus. Continuous cultivations with high cell concentration using cell recycling were performed utilizing a hollow fiber ultrafiltration module to separate cells from the filtrate broth. The continuous cultures without cell recycling and the batch cultivations were performed conventionally. The highest productivity was attained in the continuous cultivation with cell recycling (22.2 mg.L-1.h-1). The highest CA concentration was obtained in the batch process (470 mg.L-1.h-1). Tipo: Info:eu-repo/semantics/article Palavras-chave: Clavulanic acid; Streptomyces clavuligerus; Continuous culture. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400012 Evaluation of different media for the production of cephalosporins by Streptomyces clavuligerus ATCC 27064 52 Antonio,Tatiana; Bellão,Carolina; Corrêa,Tatiana; Cavallieri,André Pastrelo; Badino,Alberto Colli; Araujo,Maria Lucia Gonsales da Costa. The aim of this work was to compare the composition of a complex and soluble culture medium to eight other media described in the literature through the batch cultivation in a conventional bench-scale bioreactor for the production of cephamycin C by a wild strain of Streptomyces clavuligerus. The proposed medium resulted in an antibiotic production 1.5 to 7.5 times higher than the other culture media. Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-lactam antibiotic; Streptomyces clavuligerus; Lysine metabolism; Complex medium. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600003 PARTIAL CHARACTERIZATION OF PROTEASES FROM STREPTOMYCES CLAVULIGERUS USING AN INEXPENSIVE MEDIUM 58 Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Duarte,Helena Simões; Tambourgi,Elias Basile; Melo,Eduardo Henrique Magalhães de; Silva,Valdinete Lins; Porto,Ana Lúcia Figueiredo; Lima Filho,José Luiz de. The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium sulphate precipitation. Enzymes from the two strains retained 37% of their initial activities at pH 8.0 after 2 h incubation at 25ºC. Enzyme half-life at pH 8.0 and 60ºC was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and 8.4 and 8.0 for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Extracellular proteases; Thermal stability; PH. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300010 Screening of medium constituents for clavulanic acid production by Streptomyces clavuligerus 58 Rodrigues,Kaio César da Silva; Souza,Arianne Tairyne de; Badino,Alberto Colli; Pedrolli,Danielle Biscaro; Cerri,Marcel Otavio. ABSTRACT Clavulanic acid is a β-lactam compound with potent inhibitory activity against β-lactamases. Studies have shown that certain amino acids play essential roles in CA biosynthesis. However, quantitative evaluations of the effects of these amino acids are still needed in order to improve CA production. Here, we report a study of the nutritional requirements of Streptomyces clavuligerus for CA production. Firstly, the influence of the primary nitrogen source and the salts composition was investigated. Subsequently, soybean protein isolate was supplemented with arginine (0.0–3.20 g L-1), threonine (0.0–1.44 g L-1), ornithine (0.0–4.08 g L-1), and glutamate (0.0–8.16 g L-1), according to a two-level central composite rotatable design. A medium containing... Tipo: Info:eu-repo/semantics/article Palavras-chave: Clavulanic acid; Streptomyces clavuligerus; Ferrous sulfate; Amino acids supplementation. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400832 Protoplast formation and regeneration from Streptomyces clavuligerus NRRL 3585 and clavulanic acid production 58 Carneiro-da-Cunha,Maria das Graças; Lima Filho,José Luiz de; Campos-Takaki,Galba Maria de. Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/mL), in an osmotic solution for 90 min at 28ºC. The frequency of regenerated protoplasts in the lag phase was 1.7x10³ CFU/mL (28.97%), in the beginning of the exponential phase 0.4x10² CFU/mL (31.67%), in the exponential growth phase 2.5x10³ CFU/mL (46.30%) and 1.0x10(5) CFU/mL in stationary phase (48.45%). Antibiotic production and activity of regenerated protoplasts were observed in all phases, except in the lag phase. The protoplast formation... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Protoplast; Clavulanic acid. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400014 Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1 58 Thumar,Jignasha T.; Singh,Satya P.. An alkaliphilic and salt- tolerant actinomycete, Streptomyces clavuligerus strain Mit-1, was isolated from Mithapur, the western coast of India. The organism was Gram-positive, having filamentous, long thread like structure. The sporulation started after two days of growth and the optimum level of alkaline protease (130 U/ml) was produced during the early stationary phase. The strain could grow and produce protease with 0-10% NaCl (w/v), the optimum being 5% NaCl (w/v). Growth and protease production was optimum at pH 9 with substantial decline at neutral pH. Sucrose and gelatin were the best carbon and nitrogen sources respectively, whereas gelatin broth was the preferred medium for protease production. Mit-1 produced substantial protease with various... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Salt-tolerant actinomycetes; Alkaline protease; Enzymatic potential; Protease optimization. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400033 Clavulanic acid production by the MMS 150 mutant obtained from wild type Streptomyces clavuligerus ATCC 27064 58 Vasconcelos,Eliton da Silva; Lima,Vanderlei Aparecido de; Goto,Leandro Seiji; Cruz-Hernández,Isara Lourdes; Hokka,Carlos Osamu. Clavulanic acid (CA) is a powerful inhibitor of the beta-lactamases, enzymes produced by bacteria resistants to penicillin and cefalosporin. This molecule is produced industrially by strains of Streptomyces clavuligerus in complex media which carbon and nitrogen resources are supplied by inexpensive compounds still providing high productivity. The genetic production improvement using physical and chemical mutagenic agents is an important strategy in programs of industrial production development of bioactive metabolites. However, parental strains are susceptible to loss of their original productivity due genetic instability phenomenona. In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are compared with the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Clavulanic acid; Streptomyces clavuligerus; Mutation; Lipase. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400005 Registros recuperados: 7 Primeira ... 1 ... Última

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