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Registros recuperados: 29 | |
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Díaz,Rubén; Téllez-Téllez,Maura; Sánchez,Carmen; Bibbins-Martínez,Martha D; Díaz-Godínez,Gerardo; Soriano-Santos,Jorge. |
Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lacc4, Lacc6, Lacc9 and Lacc10) in these cultures were also studied. Results: The highest laccases activity was obtained in cultures grown at initial pH of 4.5 and the lowest in cultures grown at initial pH of 8.5. Isoenzyme profiles were different in all the cases. Lacc1, Lacc4, Lacc6 and Lacc10 were expressed in all the cultures. Conclusions: The initial pH of the growing medium is an important factor for regulating the expression of laccase genes, having an... |
Tipo: Journal article |
Palavras-chave: Laccases; Pleurotus ostreatus; Submerged fermentation; Zymogram. |
Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400006 |
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Ruiz, Nicolas; Dubois, Nolwenn; Wielgosz Collin, Gaetane; Du Pont, Thibaut; Berge, Jean-pascal; Pouchus, Yves-francois; Barnathan, Gilles. |
A marine-related Trichoderma longibrachiatum strain isolated from mussels in a farming shellfish area was investigated for total lipid (TL) production, total lipid fatty acids (TLFA), and phospholipid fatty acids (PLFA). Fungal biomass was produced from two different marine-like culture ways, on agar surface and submerged fermentations (ASF and SmF, respectively), allowing useful comparisons. ASF produced a rather higher biomass amount than SmF with similar TL content. All fatty acids (FA), identified by gas chromatography-mass spectrometry (GC-MS), were ranged from 14 to 30 carbon atoms. Similar FA compositions were found in TL and phospholipids (PL) from biomasses obtained by both fermentation systems, including oleic (up to 15.3% of total FA mixture for... |
Tipo: Text |
Palavras-chave: Conjugated fatty acids; Fatty acids; Lipids; Submerged fermentation; Agar surface fermentation; Trichoderma longibrachiatum. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4199.pdf |
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PAULA,CAIO C.P. DE; MONTOYA,QUIMI V.; MEIRELLES,LUCAS A.; FARINAS,CRISTIANE S.; RODRIGUES,ANDRÉ; SELEGHIM,MIRNA H.R.. |
Abstract Abstract: Isolation and screening of new fungal strains from extreme and understudied environments, such as caves, is a promising approach to find higher yields enzyme producers. Cellulolytic fungal strains isolated from a Brazilian cave were evaluated for their enzymatic production after submerged (SmF) and solid-state fermentation (SSF). After SmF, three strains were selected for their high enzymatic activities: Aspergillus ustus for endoglucanase (4.76 U/mg), Talaromyces bruneus for β-glucosidase (11.71 U/mg) and Aspergillus sp. (CBMAI 1926) for total cellulase (1.70 U/mg). After SSF, these strains, showed better yields compared to the reference strain Aspergillus niger 3T5B8. Aspergillus sp. (CBMAI 1926) stood out as a new species that... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulases; Fungu’s cave; Solid state fermentation; Submerged fermentation; Subterranean environment. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500601 |
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Silveira,Guilherme Garcia da; Oliveira,Gustavo Monteiro de; Ribeiro,Eloizio Julio; Monti,Rubens; Contiero,Jonas. |
The aim of this work was to study the effect of carbon and nitrogen sources in submerged fermentation, and the casein effect on solid-state fermentation on rennin production by Mucor miehei. Biomass peaks reached 6.7; 8.1 and 8 g/L and enzymatic production peaks of 1,066; 857 and 480 Soxhlet Units (S.U.) for glucose concentration of 18; 25 and 35 g/L respectively. Flasks with baffles showed biomass peaks of 6.7; 8.3 and 10 g/L and enzyme activity peaks of 648; 279 and 300 S.U. for the same glucose concentration. The values of 923 and 667 S.U. were obtained when corn steep liquor and Proflo (Cottonseed Nutrients from Traders ®) were used. In SSF system the enzymatic activities were 414, 264 and 167 S.U., when using HCl 0.2 N, 0.3 N and 0.4 N respectively.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mucor miehei; Rennin; Clotting activity; Casein; Solid-state fermentation; Submerged fermentation. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800009 |
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Vandenberghe,Luciana P. S; Soccol,Carlos R; Pandey,Ashok; Lebeault,Jean-Michel. |
Citric acid is the most important organic acid produced in tonnage and is extensively used in food and pharmaceutical industries. It is produced mainly by submerged fermentation using Aspergillus niger or Candida sp. from different sources of carbohydrates, such as molasses and starch based media. However, other fermentation techniques, e.g. solid state fermentation and surface fermentation, and alternative sources of carbon such as agro-industrial residues have been intensively studied showing great perspective to its production. This paper reviews recent developments on citric acid production by presenting a brief summary of the subject, describing micro-organisms, production techniques, and substrates, etc. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citric acid; Aspergillus niger; Submerged fermentation; Solid state fermentation; Substrates. |
Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300001 |
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Alberton,Luiz Romulo; Vandenberghe,Luciana Porto de Souza; Assmann,Ricardo; Fendrich,Ricardo Cancio; Rodriguéz-León,José; Soccol,Carlos Ricardo. |
The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptomyces; Xylanase; Submerged fermentation; Solid-state agro-industrial fermentation; Residues. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022 |
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Lima,Laisy Garcia Ribeiro; Gonçalves,Márcia Monteiro Machado; Couri,Sonia; Melo,Verônica Ferreira; Sant’Ana,Gizele Cardoso Fontes; Costa,Antônio Carlos Augusto da. |
Abstract This study aimed to evaluate the effects of variables on the process of lipases production by Aspergillus niger C by submerged fermentation (SmF). The production assays were performed in shake flasks for 72 hours at 150 rpm and 32°C. First, a fractional factorial design 25-1 (FFD) was carried out to evaluate the effect of the following process variables: sucrose, ammonium sulphate, soybean oil, yeast extract concentration and pH. After the selection of the variables that significantly influenced the lipase production, a central composite rotational design 22 (CCRD) was used, aiming to find the most favorable operational conditions. The selected assay condition (15.0 g.L-1 sucrose, 4.0 g.L-1 ammonium sulphate, 4.0 g.L-1 soybean oil and 1.0 g.L-1... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipases; Submerged fermentation; Aspergillus nige; Experimental design.. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100214 |
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Nascimento,Rodrigo Pires do; Alves Junior,Nelson; Coelho,Rosalie Reed Rodrigues. |
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptomyces malaysiensis; Brewer's spent grains; Corn steep liquor; Proteinases; Submerged fermentation. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400020 |
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Smaniotto,Alessandra; Skovronski,Aline; Rigo,Elisandra; Tsai,Siu Mui; Durrer,Ademir; Foltran,Lillian Liva; Paroul,Natália; Luccio,Marco Di; Oliveira,J. Vladimir; Oliveira,Débora de; Treichel,Helen. |
Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificity for short chain alcohols and fatty acids. The precipitated extract showed a good thermal stability, higher than that for crude enzymatic extracts. The 'CC' and 'CP' enzymes presented high activities after exposure to pH 6.5 and 40 ºC. On the other hand, the 'IC' and 'IP' extracts kept their activities in a wide range of pH memory but presented preference for higher reaction temperatures. Preliminary... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporidiobolus pararoseus; 'synthetic lipase'; Submerged fermentation; Enzyme characterization. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100043 |
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Smaniotto,Alessandra; Skovronski,Aline; Rigo,Elisandra; Tsai,Siu Mui; Durrer,Ademir; Foltran,Lillian Liva; Di Luccio,Marco; Oliveira,J. Vladimir; Oliveira,Débora de; Treichel,Helen. |
The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the results obtained in the first experimental design (2(4-1)), yeast extract and NaCl concentrations were tested to further optimization by response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0, 6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporidiobolus pararoseus; Experimental design; 'synthetic lipase'; Submerged fermentation. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400033 |
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Registros recuperados: 29 | |
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