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Working Optimally with Serial Sections in Glycol Methacrylate Resin BABT
Leitão,Carlos André Espolador.
ABSTRACT The present work presents an optimized form for distributing serial sections in a glycol methacrylate slide set. This consists of filling the first row with sections from each slide, thus proceeding in the same way with the other rows. Therefore each slide will contain sections of three distinct sample segments.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytology; Histology; Historesin; Microscopy; Plant anatomy; Technique.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100416
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Radioautographology: the proposal of a new concept BJMBR
Nagata,T..
A new concept termed "radioautographology" is advocated. This term was synthesized from "radioautography" and "ology", expressing a new science derived from radioautography. The concept of radioautographology (RAGology) is that of a science whose objective is to localize radioactive substances in the biological structure of objects and to analyze and study the significance of these substances in the biological structure. On the other hand, the old term radioautography (RAG) is the technique used to demonstrate the pattern of localization of various radiolabeled compounds in specimens. The specimens used in biology and medicine are cells and tissues. They are fixed, sectioned and placed in contact with the radioautographic emulsions, which are exposed and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Light microscopy; Electron microscopy; Radioautography; Technique; Application.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800001
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Transtracheal puncture: a forgotten procedure BJMBR
Almeida,E.P.; Almeida,A.C.; Almeida,F.F.; Montessi,J.; Gomes,C.A.; Ferreira,L.E.V.V.C..
Transtracheal puncture has long been known as a safe, low-cost procedure. However, with the advent of bronchoscopy, it has largely been forgotten. Two researchers have suggested the use of α-amylase activity to diagnose salivary aspiration, but the normal values of this enzyme in tracheobronchial secretions are unknown. We aimed to define the normal values of α-amylase activity in tracheobronchial secretions and verify the rate of major complications of transtracheal puncture. From October 2009 to June 2011, we prospectively evaluated 118 patients without clinical or radiological signs of salivary aspiration who underwent transtracheal puncture before bronchoscopy. The patients were sedated with a solution of lidocaine and diazepam until they reached a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transtracheal puncture; Aspiration; Complications; Technique; Amylase; Report values.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800725
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Histoire des engins et techniques de pêche ArchiMer
Beucher, Jean-paul; Deschamps, Gerard; Peronnet, Isabelle; Duhamel, Erwan; Barthelemy, Patricia.
L'homme a rapidement diversifié ses engins de pêche et on remarquera que les techniques alternatives qui sont actuellement pressenties pour remplacer le chalut prépondérant, sont des techniques très anciennes.
Tipo: Text Palavras-chave: Chalut; Senne; Filet; Casier; Drague; Pêche; Capture; Technique; Engin de pêche; Histoire; Evolution; Fishing; Capture; Technics; Fishing gear; History; Evolution.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00002/11355/7928.pdf
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Comparaison de deux méthodes de prélèvement pour le dénombrement des larves de Crassostrea gigas en zone conchylicole ArchiMer
His, Edouard; Borel, Michel; Robert, Rene; Laborde, Jean-louis.
La numération des larves d'huître dans le plancton renseigne les ostréiculteurs sur la date de pose des collecteurs. La technique utilisée est très ancienne (BOURY, 1928) ; pour chaque station trois prélèvements par traits de filets pendant 10 minutes sont effectués : deux en surface (filet 200 et filet 130) et un à 1 mètre de profondeur (filet 130). Une méthode par pompage avec mesure instantanée du volume d'eau filtrée a été mise au point (prélèvements en surface et à 1 mètre de profondeur, et rétention des véligères sur filets 200). La comparaison des deux techniques met en évidence l'inadaptation de l'ancienne méthode. Le choix de la porosité (70 µm pour les filets 200) est mal adapté à la récolte des larves D (stade 1) dont le nombre peut être sous...
Tipo: Text Palavras-chave: Numération; Technique; Reproduction; Crassostrea gigas; Larves; Naissain; Recrutement; Ostréiculture.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-6444.PDF
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Les marinades des produits de la mer ArchiMer
Knockaert, Camille.
Marinade is a light pickling brine, eventually spiced or sugared, acidified with vinegar or other allowed organic acid. Marinades are half-preserved food that do not have undergo a preliminary treatment in order to eliminate all micro-organisms. The principes of marinading, the importance of sea-products quality, their acidification , and the subsequent pasteurization are explained in this book. Some examples of industrial recipes are given. Legal recommendations are given as annexes.
Tipo: Text Palavras-chave: Seafood /Fishery industry legislation; Commercial species; Processed fishery products; Conditionnement; Technique; Marinage; Valorisation; Méthodologie; Conservation; Traitement produit pêche.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/rapport-630.pdf
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Essais d'élevage de moules sur tubes amovibles dans le Pertuis Breton ArchiMer
Dardignac-corbeil, Marie-jose.
Lors de la réunion interministérielle tenue à l'Hôtel Matignon le 25 juillet 1980, il fut décidé que la production conchylicole française devait être développée afin de résorber le déficit commercial extérieur pour les moules et d'accroître nos ventes d'huîtres à l'étranger. De 1979 à 1981, la production mytilicole a évolué entre 60 000 et 80000 tonnes et les importations entre 23 600 et 35 700 tonnes; en 1981, le déficit se montait à plus de 81 millions de francs. Pour développer la production, trois voies sont possibles : - l'amélioration des rendements dans les zones déjà exploitées - l'extension des élevages à d'autres secteurs où les techniques habituelles peuvent être utilisées, c·'est à dire sur l'estran - la mise en valeur de sites inaccessibles...
Tipo: Text Palavras-chave: Pertuis Breton; Mytilus edulis; Elevage; Tubes amovibles; Technique; Croissance.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/00045/15585/12987.pdf
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Prégrossissement et élevage de palourdes japonaise en marais : technique entre filet ArchiMer
Zanette, Yvan; Heral, Maurice; Peyre, Roger.
Another breeding technique was worked out for Japanese clam Ruditapes philippinarum. It consists in breeding it in a large two-fold net dipped into mud.
Tipo: Text Palavras-chave: Technique; Elevage; Ruditapes philippinarum.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/acte-2942.pdf
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Diversification des techniques de pêche artisanales en corse. Pêche de la crevette Pandalide plesionika edwards II. Technique - prospection - étude biologique - commercialisation ArchiMer
Guennegan, Yvon.
INTRODUCTION : « The fishing industry is still flourishing » but in Corsica as elsewhere, it has already been declining; sea floors are getting depleted. Several vessels have laid up. Where are we in 1989? Corsica fishing is strongly seasonal. The bulk of the activity takes place from spring time to early fall and focuses on bottom fish and sea crayfish. Three types of fishing can be identified: - Trawl fishing on the eastern coast plateau, (16 trawlers). - Trammel fishing to catch rock fish and sea crayfish on the western coast of the island (300 low power and tonnage ships, i.e. 50 KW and 4TJB). - Pond fishing (a few small units). Total catching amounts to roughly 1 000 tons. The fishing fleet is very dispersed along 1 080 km of coast and sometimes faces...
Tipo: Text Palavras-chave: Marketing; Biological study; Fish shrimp; Prospection; Technique; Pandalide plesionika edwards II; Corsica; Artisanal fishings.
Ano: 1990 URL: https://archimer.ifremer.fr/doc/1990/rapport-1759.pdf
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Dénombrement rapide des coliformes dans les coquillages. Efficacité des milieux A1 -M & MUG ArchiMer
Montfort, Patrick.
Le dénombrement des coliformes fécaux constitue la référence en matière d'évaluation de la salubrité des zones conchylicoles, de la qualité des bivalves destinés à la consommation humaine. Chacun jugera de l'intérêt et de l'efficacité que peut présenter une investigation diligente en ce domaine. Or, les techniques microbiologiques utilisées à l'heure actuelle se révèlent incompatibles avec ces objectifs voire avec les impératifs des laboratoires. Nous avons donc entrepris de tester deux nouveaux milieux (A1-M et MUG) afin de juger de leur fiabilité. L'étude statistique fait appraître une corrélation étroite entre le milieu A1-M et le bouillon BLBV, nous confortant  dans la poursuite de cette application à savoir l'évaluation de la fréquence du nombre de...
Tipo: Text Palavras-chave: Conchyliculture; Salubrité; Microbiologie; Coliformes; Technique.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00430/54161/55493.pdf
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Elaboration d'une technique d'évaluation des stocks conchylicoles : biomasses en élevage en 2001, et développement d'une méthode simplifiée à l'usage de la profession ArchiMer
Le Moine, Olivier; Razet, Daniel; Geairon, Philippe.
La connaissance des biomasses en élevage est un atout majeur en terme d'aménagement (restructurations, nettoyages collectifs de zones, etc . .. ) ainsi que dans la gestion de la campagne de commercialisation. La Section Régionale Conchylicole, ainsi que L'Organisation des Producteurs d'huîtres de Marennes Oléron, conscients des avantages qu'apporte cette connaissance, a contractualisé avec le Laboratoire Conchylicole de Poitou Charentes la mise au point de techniques plus rapides, et simplifiées à usage de la profession en ce qui concerne l'évaluation des stocks commercialisables dans la campagne. Les évaluations de stocks conchylicoles font partie des travaux que les laboratoires côtiers de l'lFREMER accomplissent de manière périodique, tous les cinq ans...
Tipo: Text Palavras-chave: Conchyliculture; Estimation stock; Technique; Biomasse; Echantillonnage; Bassin de Marennes Oléron.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00095/20613/18248.pdf
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Compte-rendu des résultats d'analyses effectuées en histologie - Année 1995 ArchiMer
Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno.
Dans le cadre du Réseau zoosanitaires CEE (huître plate) et suivi d'autres espèces, 890 individus provenant des zones 5, 6 et 7 ont été examinés sur l'ensemble de l'année 1995 par différentes techniques : 472 analyses sous coupes histologiques, 328 analyses sur appositions d'organes, 90 analyses sur milieu au thioglycolate.
Tipo: Text Palavras-chave: Pathologie; Analyse; Histologie; Technique; Bivalves; Ostrea edulis; Huître plate; Mytilus edulis; Ruditapes philippinarum; Ruditapes decussatus; Cerastoderma edule; Mortalité.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00044/15504/12877.pdf
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Observations sur le cycle complet d'élevage de palourdes et de clams en surélévation ArchiMer
Latrouite, Daniel; Claude, Serge.
First rearing attempts with the european clams Ruditapes decussatus, the pacific clams Ruditapes philippinarum and the clam Mercenaria mercenaria have been realized by placing the bood into grating cages above the ground. This technic permits a good survival during the so-called "pre-growth" stage. However, if rearing of juveniles is persued beyond 2 centimeters, growth is strongly delayed and mortality increases due to valve distortion and shell infection by parasitic worms.
Tipo: Text Palavras-chave: Elevage; Palourde; Ruditapes philippinarum; Ruditapes decussatus; Clams; Mercenaria mercenaria; Technique.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/00021/13261/10308.pdf
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A bioassay method for black flies (Diptera: Simuliidae) using larvicides Neotropical Entomology
Araújo-Coutinho,Carlos J.P.C.; Figueiró,Ronaldo; Viviani,Andréia P.; Nascimento,Érika S.; Cavados,Clara F.G..
This note presents an alternative method for bioassays using black fly larvae, with which we intend to provide a simpler but effective tool for accessing data on larvicide efficacy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Efficacy; Technique.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300022
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Evaluation of lugol solution used for counting rumen ciliates Zoologia
D'Agosto,Marta; Carneiro,Maria Elisa.
The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rumen ciliate; Quantitative evaluation; Lugol solution; Technique.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000300011
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