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| Cario, Anais; Mizgier, Alex; Thiel, Axel; Jebbar, Mohamed; Oger, Phil M.. |
| Most Thermococcales accumulate di-myo-inositol-phosphate (DIP) as an organic solute as a response to heat stress. We have studied the accumulation of this osmolyte in the high-hydrostatic pressure adapted hyperthermophile Thermococcus barophilus. We found no accumulation of DIP under any of the stress conditions tested, although this archaeon harbors the 3 DIP synthesis genes. Lack of synthesis is due to the lack of expression of TERMP_01135 coding for the second step of DIP synthesis. In contrast to other species, the T. barophilus synthesis operon is interrupted by a four gene locus, in reverse orientation. Restoring an operon like structure at the DIP locus restored DIP synthesis, but did not have an impact on growth characteristics, suggesting that... |
| Tipo: Text |
Palavras-chave: High hydrostatic pressure; Heat stress; Di-myo-inositol-phosphate; Thermococcus barophilus. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38113/37216.pdf |
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