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Registros recuperados: 32 | |
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Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle. |
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the... |
Tipo: Text |
Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf |
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Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle. |
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010... |
Tipo: Text |
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf |
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Dang, C.; Gonzalez, P.; Mesmer-dudons, N.; Bonami, J-r; Caill-milly, Nathalie; De Montaudouin, X.. |
Recently, Manila clam, Ruditapes philippinarum, populations have suffered mortalities in Arcachon Bay (SW France). Mortality was associated with extensive lesions of the posterior adductor muscle, which become progressively brown and calcified. Ultrastructural observations by transmission electron microscopy revealed tissue degradation with necrotized muscle fibres and granulocytomas. Unenveloped virus-like particles (VLPs) were detected in muscle, granulocytic, epithelial and rectal cells. VLPs were abundant in the extracellular space, in the cytoplasm (free or enclosed in vesicles) and in the nucleoplasm of granulocytes. Nuclei and mitochondria of granulocytes displayed changes which suggested reactive oxygen species production and apoptosis induction.... |
Tipo: Text |
Palavras-chave: Adductor muscle; Apoptosis; Brown muscle disease; Necrosis; Ruditapes philippinarum; Transmission electron microscopy. |
Ano: 2009 |
URL: https://archimer.ifremer.fr/doc/00000/11130/7991.pdf |
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Catroxo,M. H. B; Bersano,J. G; Martins,A. M. C. P. F; Petrella,S; Portugal,M. A. S. C; Souza,O. S. |
The myxomatosis is a contagious worldwide disease caused by poxvirus which infects domestic and wild rabbits. In the present study we present two distinct outbreaks of myxomatosis when raising rabbits, one for commercial purpose of production of meat and skins and, another one for the commercialization of ornamental rabbits. The observed signs were ocular, auricular, nasal, testis lesions and many times scattered throughout the body of the animals. The lesions were characterized by formation of nodules that by palpation disclosed gummy or gelatinous aspect. At the transmission electron microscopy, all the skin and crust samples were analyzed by negative staining technique. A great number of particles with morphology similar to the poxvirus, some enveloped... |
Tipo: Journal article |
Palavras-chave: Myxomatosis; Poxvirus; Rabbits; Transmission electron microscopy. |
Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200039 |
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Catroxo,M. H. B; Miranda,L.B; Lavorenti,A; Petrella,S; Melo,N. A; Martins,A. M. C. P. R. F. |
Coronaviruses are enveloped, positive-stranded RNA, belongs to the Nidovirales order, Coronaviridae family and are classified into three groups. Groups 3a and 3c comprising the avian coronaviruses. They are worldwide in distribution and can cause respiratory, enteric and in some cases hepatitis and neurological diseases in a wide variety of animals, resulting in economic losses to breeders and breeding. In this study, we describe an outbreak of diarrhea in a herd of capybaras found in the city of Piracicaba, São Paulo, Brazil. The animals had sudden symptoms of anorexia, profuse and watery diarrhea, yellowish or greenish, dehydration, weight loss, sharp and twisting of limbs leading to death in less than a week, mostly weaned and with an age of three... |
Tipo: Journal article |
Palavras-chave: Coronavirus; Capybaras; Transmission electron microscopy. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200035 |
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Catroxo,M. H. B; Pongiluppi,T; Melo,N. A; Milanelo,L; Petrella,S; Martins,A. M. C. P. F; Rebouças,M. M. |
Avianpox is a highly contagious disease infecting both commercial and wild birds, causing great damages to breeders and breeding. Caused by DNA viruses of the family Poxviridae, genus Avipoxvirus, if manifest through 3 forms, cutaneous, diphtheric and septicemic. In June 2003, during illegal commercialization of Brazilian birds, 800 wild birds (Paroaria dominicana, Sporophila caerulescens and Sporophila albogularis) were apprehended and being forwarded to the CRAS (Wild Animals Recovery Center), Tietê Ecological Park. After one month, birds presented cutaneous lesions in the beak and feet and anorexia, emaciation, locomotion difficulties, diarrhea, dehydration and death. Among the 800 birds, 500 died and 40 these (15 Paroaria dominicana, 15 Sporophila... |
Tipo: Journal article |
Palavras-chave: Poxvirus; Paroaria dominicana; Sporophila caerulescens; Sporophila albogularis; Transmission electron microscopy. |
Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200043 |
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Benigno,Maria Ivone Mendes; Liberti,Edson Aparecido; Conde Jr,Airton Mendes; de Sousa Leal,Noélia Maria; Lopes Rodrigues,Karla Emanuelle; Mota,Vanessa Benigno; Amstalden,Eliane Maria Ingrid. |
Twelve adult male Wistar rats (220 g average weight) were divided in 3 experimental groups: GI -15. GII 30 and GIII 60 days, after mandibular molar extraction, with three experimental animals and one control per group. Qualitatively, ultrastructural changes of protein filaments from myofibrils of these muscles and ipsilateral to the extractions were observed. Ultrastructure asymmetry and disorganization of Z line and I band, in the experimental group GII, of Medial Pterigoid muscle (MPT) were observed. The temporomandibular dysfunction, stimulated by the unilateral extractions of mandibular molars in rats may lead to modifications in the Z line and I band, which showed to be sensitive to this dysfunction. Changes in the MPT muscle, probably related to... |
Tipo: Journal article |
Palavras-chave: Skeletal muscular fibre; Masticator muscles; Occlusal alterations; Temporomandibular joint; Transmission electron microscopy. |
Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200007 |
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Catroxo,M. H. B; Taniguchi,D. L; Melo,N. A; Milanelo,L; Petrella,S; Alves,M; Martins,A. M. C. R. P. F; Rebouças,M. M. |
The barn-owl (Tyto Alba) and striped-owl (Rhinoptynx clamator) belong respectively to the families Tytonidae and Strigidae. Avian paramyxoviruses have been isolated from a variety of species of wild and domestic birds wordlwide causing diverse clinical symptoms and signs. Paramyxoviruses belong to the family Paramyxoviridae and Avulovirus genus, including nine serotypes (APMV 1 to 9). The lymphoid leukosis is a retrovirus-induced neoplasia. The avian retroviruses belong to the Retroviridae family and to the Alpharetrovirus genus. Coronaviruses can cause respiratory and enteric disease in several species of birds. They belong to the Coronaviridae family and to the groups 3a e 3c. In this study, we describe the presence of viruses in four owls, two barn owls... |
Tipo: Journal article |
Palavras-chave: Paramyxovirus; Coronavirus; Retrovirus; Owls; Transmission electron microscopy. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200047 |
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Revajová, Viera; Loószová, Adriana; Goldová, Maria; Zibrín, Martin; Herich, Robert; Levkut, Mikulas. |
In order to morphological study of non-specific host immune response, cross-species experimental infection of Leghorn chicks with partridge specific coccidium, Eimeria procera (106 oocyst/per birds) was done. Schizonts of E. procera were histologically observed in all the parts of small intestine and caeca of chicks. Heterophilic and lymphocytic infiltration in lamina propria, and damage of villi intestine were observed. Schizonts were studied by electron microscope after 12, 30, and 60 hours post-infection (h pi). Results demonstrated immature and structurally damaged schizonts at 12 h pi, delayed maturation of first generation schizonts at 30 h pi, and phagocytosis of parasites at 60 h pi by intraepithelial lymphocytes. |
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Palavras-chave: Eimeria procera; Transmission electron microscopy; Specific and non-specific host; Intraepithelial lymphocytes. |
Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/144 |
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Alves,Eduardo; Leite,Breno; Pascholati,Sérgio Florentino; Ishida,Maria Lúcia; Andersen,Peter Craig. |
Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ultrastructure; Scanning electron microscopy; Transmission electron microscopy; Immunolabel. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200011 |
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Registros recuperados: 32 | |
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