|
|
|
|
| |
|
| |
|
|
Fabioux, Caroline; Corporeau, Charlotte; Quillien, Virgile; Favrel, Pascal; Huvet, Arnaud. |
This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas,... |
Tipo: Text |
Palavras-chave: Vasa; RNAi; Marine bivalve; Germline; Crassostrea gigas. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6641.pdf |
| |
|
|
Fabioux, Caroline; Pouvreau, Stephane; Leroux, Frederique; Huvet, Arnaud. |
The vasa gene is,a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a vasa orthologue in Crassostrea gigas (Oyvlg). OyVLG contained the eight consensus domains of the DEAD-box including those providing RNA unwinding activity. The expression pattern of Oyvlg was examined in adult oyster tissues at different reproductive stages. Its expression was restricted to germline cells both in males and females, including germinal stem cells and auxiliary cells. The expression of Oyvlg, strongest in early germ cells, decreased as the maturation... |
Tipo: Text |
Palavras-chave: RNA helicase; DEAD box; Vasa; Germline; Gametogenesis; Oyster. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-646.pdf |
| |
|
|
|