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| Registros recuperados: 40 | |
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| Martínez Aispuro, José Alfredo. |
| El presente trabajo tuvo como objetivo reducir y/u optimizar la concentración de algunos nutrimentos en las dietas de cerdos de engorda que puede resultar benéfica en la formulación de raciones. Se realizaron 3 experimentos para evaluar el nivel de lisina, el uso de alcaloides y xilanasas en dietas para cerdos de engorda. En el experimento 1 se determinaron los niveles óptimos biológicos (NOB) de lisina total para cerdos en las etapas de crecimiento y finalización en el comportamiento productivo, las características de la canal, y la concentración de urea en plasma. El NOB para ganancia diaria de peso (GDP), conversión alimenticia, y ganancia de carne magra fue 0.88% de lisina total; mientras que el NOB para consumo de alimento fue 0.961% de lisina total.... |
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Palavras-chave: Lisina; Alcaloides; Dietas con baja proteína; Xilanasas; Energía; Proteína; Lysine; Alkaloids; Low protein diets; Xylanase; Energy; Protein; Ganadería; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/2159 |
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| Villena,Gretty K; Gutiérrez-Correa,Marcel. |
| Lignocellulolytic enzyme production by Aspergillus niger was compared both in submerged fermentation (SF) and biofilm fermentation (BF) at varying water activities. Maximal filter paper activity, endoglucanase and xylanase activities were much higher in BF (2.96, 4.7 and 4.61 IU ml-1, respectively) than in SF cultures (1.71, 1.31 and 2.3 IU ml-1, respectively) but biomass yields were lower in BF than in SF (0.338 g g-1 and 0.431 g g-1, respectively). In the presence of 20% ethylene glycol (a w = 0.942) the enzyme activities decreased in both systems but BF still had higher levels (1.0, 1.0 and 2.6 IU ml-1, respectively) than SF cultures (0.6, 0.7 and 1.5 IU ml-1, respectively). An increase in xylanase specific activity of more than 2 fold (from 4.2 to 10.2... |
| Tipo: Journal article |
Palavras-chave: Biofilm; Cellulase; Ethylene glycol; Water activity; Xylanase. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100012 |
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| Zhang,Guangya; Ge,Huihua. |
| Background: Support vector machine (SVM), a novel powerful machine learning technology, was used to develop the non-linear quantitative structure-property relationship (QSPR) model of the G/11 xylanase based on the amino acid composition. The uniform design (UD) method was applied to optimize the running parameters of SVM for the first time. Results: Results showed that the predicted optimum temperature of leave-one-out (LOO) cross-validation fitted the experimental optimum temperature very well, when the running parameter C, ξ, and γ was 50, 0.001 and 1.5, respectively. The average root-mean-square errors (RMSE) of the LOO cross-validation were 9.53ºC, while the RMSE of the back propagation neural network (BPNN), was 11.55ºC. The... |
| Tipo: Journal article |
Palavras-chave: Amino acid composition; Optimum temperature; Support vector machine; Uniform design; Xylanase. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100007 |
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| Ahmed,Sibtain; Imdad,Syeda Sana; Jamil,Amer. |
| Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T.... |
| Tipo: Journal article |
Palavras-chave: C. thermophilum; Kinetics; T. harzianum; Xylanase. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300003 |
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| Dulermo, Remi; Brunel, Francois; Dulermo, Thierry; Ledesma-amaro, Rodrigo; Vion, Jeremy; Trassaert, Marion; Thomas, Stephane; Nicaud, Jean-marc; Leplat, Christophe. |
| Background The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and the hybrid hp4d promoter. Recently, new hp4d-inspired promoters have been created that couple various numbers of UAS1 tandem elements with the minimal LEU2 promoter or the TEF promoter. Three different protein-secretion signaling sequences can be used: preLip2, preXpr2, and preSuc2. Results To our knowledge, our study is the first to use a set of vectors with promoters of variable strength to produce proteins of industrial interest. We used the more conventional TEF and hp4d promoters along with five new hybrid promoters: 2UAS1-pTEF,... |
| Tipo: Text |
Palavras-chave: Yarrowia lipolytica; Protein production; RedStar2; Glucoamylase; Xylanase; Hybrid promoters. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00428/54003/55193.pdf |
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| Sousa,RF; Dourado,LRB; Lopes,JB; Fernandes,ML; Kato,RK; Nascimento,DCN; Sakomura,NK; Lima,SBP; Ferreira,GJBC. |
| ABSTRACT The present study was conducted to evaluate the performance, carcass yield, and histomorphometry of the small intestine of broilers fed yeast sugarcane supplemented with enzymatic blend from 22 to 42 days of age. Seven hundred broiler chicks were distributed in a completely randomized design in a factorial design (2x3+1), two levels of the enzyme blend (0 and 200 g/ton), three protein levels (0%, 6% and 12%) and a control diet. The level of 6% yeast showed higher feed intake and weight gain after 33 days. From 22 to 42 days there was no significant effect (p<0.05) to the use of yeast in animal performance, carcass yield and cuts. At 42 days an interaction between the factors for width and crypt depth in the duodenum and jejunum, respectively,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cane sugar; Microscopy; Metabolism; Saccharomyces cerevisiae; Xylanase. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200300 |
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| Dourado,LRB; Sakomura,NK; Barbosa,NAA; Bonato,MA; Kawuauchi,IM; Fernandes,JBK; Costa,FGP. |
| Two metabolism assays were carried out to determine corn and soybean meal metabolizable energy when enzymes were added. In the first trial, 35 cockerels per studied feedstuff (corn and soybean meal) were distributed in a completely randomized experimental design with four treatments of seven replicates of one bird each. The evaluated treatments were: ingredient (corn and soybean meal) with no enzyme addition, with the addition of an enzyme complex (xylanase, amylase, protease - XAP), xylanase, or phytase. Precise feeding method was used to determine true metabolizable energy corrected for nitrogen balance (TMEn). The use of enzymes did not result in any differences (p>0.05) in soybean meal TMEn, but phytase improved corn TMEn in 2.3% (p=0.004). In the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Forced feeding; Phytase; Protease; Xylanase. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100008 |
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| Neumann,Mikael; Leão,Guilherme Fernando Mattos; Horst,Egon Henrique; Stuani,Osmair Flavio; Sangali,Cleiton Pagliari; Castilho,Ricardo. |
| ABSTRACT The objective of this study was to evaluate the effects of supplementation of two different enzymatic complexes, fibrolytic (NSPases) or amylolytic (EXP3066) enzymes, on growth performance, digestibility, behavior, and carcass characteristics of feedlot cattle. Thirty-six ½ Angus yearling bulls with an average initial weight of 391±5.0 kg were used in this experiment blocked by weight in a completely randomized design divided in three treatments: control, NSPases, and EXP3066. The basal diet was composed of 85% whole corn grain and 15% nucleus. NSPases increased average daily gain on days 63 and 84, and gain:feed ratio on days 42, 63, and 84. Total carcass weight and carcass daily gain were improved by 4.8% and up to 6.0% with EXP3066 and NSPases... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Corn; High-energy diet; Non-starch polysaccharides; Xylanase. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100611 |
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| Santana,Yânez André Gomes; Vasconcelos,Vânia Rodrigues; Alves,Arnaud Azevêdo; Silva,Suzana Coimbra de Moura Lustosa e; Garcez,Bruno Spindola. |
| ABSTRACT The effect of treatment with xylanase and β-glucanase was evaluated for gas production and the ruminal degradation of nutrients from the hay of Tifton 85 grass and the stubble of maize, sorghum, peanut, sunflower and sesame crops. Two commercial fibrolytic enzymes were used (Dyadic xylanase PLUS - Xylanase; BrewZyme LP-β-glucanase), added to the hay at doses of 7.5 units of endoglucanase and 0.46 units of xylanase per 500 mg/gDM, for the cellulase and xylanase products respectively. The chemical composition of the hay was determined for no enzyme application and 24 hours after enzyme treatment, and the in vitro gas production and in situ microbial degradation was estimated for dry matter, organic matter, neutral detergent fibre and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arachis hypogaea; Β-glucanase; Helianthus annuus; Sesamum indicum; Sorghum bicolor; Xylanase. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000100167 |
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| Joshi,Chetna; Khare,Sunil Kumar. |
| Regulation of xylanase production in two thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile was investigated. The expression of xylanase was found to be inducible in both the cases. Various carbon sources were tested so as to identify the inducers. Soy flour and oat spelt xylan induced maximum level of xylanase in Scytalidium thermophilum and Sporotrichum thermophile respectively. Induction of xylanase in Scytalidium thermophilum led to simultaneous induction of cellulase. The zymography of enzyme preparations revealed that different carbon sources caused differential expression of multiple isoforms of xylanase in Scytalidium thermophilum, but same isoforms were expressed by Sporotrichum thermophile irrespective of the carbon source... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Induction; Isoforms; Xylanase; Biobleaching. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003 |
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| Alberton,Luiz Romulo; Vandenberghe,Luciana Porto de Souza; Assmann,Ricardo; Fendrich,Ricardo Cancio; Rodriguéz-León,José; Soccol,Carlos Ricardo. |
| The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptomyces; Xylanase; Submerged fermentation; Solid-state agro-industrial fermentation; Residues. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022 |
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| Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil. |
| ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407 |
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| Cordeiro,Carlos Alberto Martins; Martins,Meire Lelis Leal; Luciano,Angélica Bárbara; Silva,Roberta Freitas da. |
| An aerobic, thermophilic, xylanolytic bacterium was isolated from local soil. The results of 16S rRNA sequence comparisons indicated that the isolate was closely related to Bacillus caldoxylolyticus and Bacillus sp strain AK1. These organisms exhibited 94% levels of ribossomal DNA sequence homology. Studies on the xylanase characterisation from liquid cultures grown on beechwood xylan revealed that the enzyme retained 100% of activity for 2 hours at temperatures ranging from 30 to 50º C, while at 60, 70 and 100º C, 10%, 11% and 29% of the original activities were lost, respectively. The optimum pH of the enzyme was found to be between 6.5 and 7.0. After incubation of crude enzyme solution for 24 hours at 25º C and at pH 5.5 to 8.0, a decrease of about 12%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylanase; Thermophilic bacterium; Bacillus sp. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600002 |
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| Registros recuperados: 40 | |
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