Data/hora: 12/06/2024 01:08:37

Provedor de dados: | 1
País: | Brazil
Título: | ‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds
Autores: | Silva, Lílian Abadia da; Sales, Juliana de Fátima; Oliveira, João Almir; Pinho, Édila Vilela de Resende Von; Santos, Heloisa Oliveira dos; Soares, Marcos Antônio.
Data: | 2015-05-04
Ano: | 2015
Palavras-chave: | 5.01.03.00-8 drying; Germination; Isoenzymes; Respiration rate; Heat-resistant proteins Produção e beneficiamento de sementes.
Resumo: | This study aimed to investigate the desiccation tolerance of Rhamnidium elaeocarpum Reissek seeds through physiological and biochemical alterations. Fruit were collected from the municipality of Jaupaci, Goias State, Brazil, when the water content of their seeds had a 37% wet basis (w.b.) and the seeds were subsequently kept in a drying oven with air circulation at a temperature of 35ºC until a water content was reached of 20 to 12% (w.b.). The following parameters were evaluated: germination, germination speed index, emergence, emergence speed index, electrical conductivity and respiration rate. Furthermore, the electrophoretic profile of the isoenzymes: catalase, peroxidase and esterase, the enzymatic activities of endo-β-mannanase and α-amylase and the electrophoretic analysis of heat-resistant proteins were performed. A higher germination speed and respiratory rate was obtained for seeds with a water content of 12% w.b., and the activity of catalase isoforms was more pronounced in seeds with a higher degree of dehydration, which was in contrast to peroxidase, showed decreased activity. The seeds contained heat-resistant proteins of low molecular weight that ranged from 48.7 to 13.2 kDa. We found that the acquisition of drying tolerance in R. Elaeocarpum Reissek seeds is associated more with catalase than with esterase and peroxidase.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425

10.4025/actasciagron.v37i2.19425

Editor: | Universidade Estadual de Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425/pdf_67
Formato: | application/pdf

Fonte:

Acta Scientiarum. Agronomy; v. 37, n. 2 (2015); 181-189

Acta Scientiarum. Agronomy; v. 37, n. 2 (2015); 181-189

1807-8621

1679-9275