Data/hora: 09/06/2024 11:48:51

Provedor de dados: | 1
País: | Brazil
Título: | QTLs identification for characteristics of the root system in upland rice through DNA microarray
Autores: | Terra, Thiago Gledson Rios; Rodrigues, Haroldo Silva; Rangel, Paulo Hideo Nakano; Tomaz, Rafael Simões; Cruz, Cosme Damião; Borém, Aluízio.
Data: | 2016-09-02
Ano: | 2016
Palavras-chave: | 5.01.03.05-9 genomic; Oryza sativa L.; DNA chip; QTL mapping; Abiotic stress Melhoramento Vegetal.
Resumo: | The aim of this work is the construction of a genetic map and identification of quantitative trait loci (QTLs) that control characteristics of the root system of rice. We evaluated a F2:3 population composed of 150 families from the cross between the varieties IAC 165 × BRS Primavera. Genotyping was performed in the F2 population using 3,742 SNP (Single Nucleotide Polymorphism) markers. The evaluation of the root system in the F3 population was performed through a large-scale phenotyping method based on image generation with a CI-600 root scanner and on quantification through the WinRhizo® software. The experiment was arranged in a randomized block design with three replications performed under greenhouse. The variables analyzed were root length, root surface area and root volume at the depths of 5 to 25 cm and 25 to 45 cm. The SNP markers analysis allowed the construction of the genetic map with a full length of 1424 cM. The linkage group with the largest coverage area was number 3 with 270 cM (100 SNPs), followed by linkage group 1 with 249 cM (170 SNPs) and linkage group 2 with 163 cM (99 SNPs). The genetic analysis allowed the detection of QTLs for all the characteristics.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30534

10.4025/actasciagron.v38i4.30534

Editor: | Universidade Estadual de Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30534/pdf
Formato: | application/pdf

Fonte:

Acta Scientiarum. Agronomy; v. 38, n. 4 (2016); 457-466

Acta Scientiarum. Agronomy; v. 38, n. 4 (2016); 457-466

1807-8621

1679-9275

Fonte: | Direitos autorais 2016 Acta Scientiarum. Agronomy