Data/hora: 13/06/2024 08:15:30

Provedor de dados: | 1
País: | Brazil
Título: | Morphology and viability of castor bean genotypes pollen grains
Autores: | Diamantino, Maria Selma Alves Silva; Costa, Maria Angélica Pereira de Carvalho; Soares, Taliane Leila; Morais, Daniel Vieira; Silva, Simone Alves; Souza, Everton Hilo de.
Data: | 2016-01-01
Ano: | 2016
Palavras-chave: | Fitotecnia Ricinus communis L.; In vitro germination; Colorimetric analysis; Pollen tube Melhoramento Vegetal.
Resumo: | The objective of this work was to characterize the morphology and viability of the pollen of 15 genotypes of castor bean (Ricinus communis L.) and to generate information that can assist in the selection of highly promising male parents for future use in genetic improvement programs aimed at producing seeds for oil extraction. Acetolysis and scanning electron microscopy was used to characterize the morphology of the pollen. The viability of the pollen grains was estimated by in vitro germination and colorimetric analysis (acetocarmine 2% and 2, 3, 5-triphenyltetrazolium chloride 1%). For the in vitro germination, pollen grains were grown in 10 types of solidified culture medium consisting of different concentrations of sucrose, boric acid, calcium nitrate, magnesium sulfate and potassium nitrate. The pollen grains had the following characteristics: medium size, isopolar and subspheroidal shape, radial symmetry, circular ambit, 3-colporate, elongated endoapertures, tectate exine and granulated sexine. The acetocarmine dye overestimated pollen viability. The media M5 and M8 were the most efficient at promoting the germination of pollen grains. The studied genotypes had high levels of viability and can therefore be used as male parents in genetic improvement programs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25981

10.4025/actasciagron.v38i1.25981

Editor: | Universidade Estadual de Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25981/pdf_115
Formato: | application/pdf

Fonte:

Acta Scientiarum. Agronomy; v. 38, n. 1 (2016); 77-83

Acta Scientiarum. Agronomy; v. 38, n. 1 (2016); 77-83

1807-8621

1679-9275