Data/hora: 16/06/2024 09:01:41

Provedor de dados: | 102
País: | Brazil
Título: | Transgenic passionfruit expressing RNA derived from Cowpea aphid-borne mosaic virus is resistant to passionfruit woodiness disease
Autores: | Alfenas,Poliane F.; Braz,Antonio Sérgio K.; Torres,Leonardo B.; Santana,Enilton N.; Nascimento,Ana Verônica S. do; Carvalho,Murilo G. de; Otoni,Wagner C.; Zerbini,F. Murilo.
Data: | 2005-02-01
Ano: | 2005
Palavras-chave: | Passionfruit woodiness virus; PWV; Potyvirus; Resistance.
Resumo: | Sixteen transgenic yellow passionfruit (Passiflora spp.) plants (R0) were obtained which express a non-translatable transgenic RNA corresponding to the 3' region of the NIb gene and the 5' region of the CP gene, derived from the genome of a Brazilian isolate of Cowpea aphid-borne mosaic virus (CABMV). The transgenic plants were propagated by stem cuttings and challenged by sap inoculation with isolates CABMV-MG1 and CABMV-PE1. One transgenic plant (TE5-10) was resistant to the isolate CABMV-MG1, but susceptible to CABMV-PE1. The remaining transgenic plants developed systemic symptoms, equal to non-transformed plants, when inoculated with either isolate. The absence of virus in TE5-10 plants was confirmed by indirect ELISA. Transcription analysis of the transgene demonstrated that the TE5-10 plant did not accumulate transgenic mRNA, even before inoculation. After inoculation, viral RNA was only detected in plants inoculated with CABMV-PE1. These results confirm that the transgenic plant TE5-10 is resistant to isolate CABMV-MG1, and suggest that the resistance mechanism is post-transcriptional gene silencing, which is already activated in the transgenic plants before virus inoculation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100006
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582005000100006
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.30 n.1 2005
Fonte: | info:eu-repo/semantics/openAccess