Data/hora: 16/06/2024 07:09:01

Provedor de dados: 	102	
País: 	Brazil	
Título: 	Expression of Grapevine leafroll-associated virus 3 coat protein gene in Escherichia coli and production of polyclonal antibodies	
Autores: 	Fajardo,Thor V.M.; Barros,Danielle R.; Nickel,Osmar; Kuhn,Gilmar B.; Zerbini,F. Murilo.	
Data: 	2007-12-01	
Ano: 	2007	
Palavras-chave: 	Grapevine; GLRaV-3; Recombinant protein; Serology; Antibodies.	
Resumo: 	Grapevine leafroll-associated virus 3 (GLRaV-3), the main viral species of the grapevine leafroll complex, causes yield and quality reduction in grapes (Vitis spp.). The coat protein gene was RT-PCR-amplified from total RNA extracted from infected grapevine leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pRSET-C expression vector. The recombinant plasmid was used to transform Escherichia coli BL21:DE3 and express the capsid protein. The coat protein, fused to a 6 His-tag, was purified by affinity chromatography using an Ni-NTA resin. The identity of the purified protein was confirmed by SDS-PAGE and Western blot. The in vitro-expressed protein was quantified and used for rabbit immunizations. The antiserum was shown to be sensitive and specific for the detection of GLRaV-3 in grapevine extracts in Western blot and DAS-ELISA assays, with no unspecific or heterologous reactions against other non-serologically related viruses being observed.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000600007	
Editor: 	Sociedade Brasileira de Fitopatologia	
Relação: 	10.1590/S0100-41582007000600007	
Formato: 	text/html	
Fonte: 	Fitopatologia Brasileira  v.32 n.6 2007	
Fonte: 	info:eu-repo/semantics/openAccess