Data/hora: 10/06/2024 14:00:27

Provedor de dados: | 102
País: | Brazil
Título: | Identification of rapd marker linked to blast resistance gene in a somaclone of rice cultivar Araguaia
Autores: | ARAÚJO,LEILA G.; PRABHU,ANNE S.; FILIPPI,MARTA C.
Data: | 2002-04-01
Ano: | 2002
Palavras-chave: | Molecular markers; Disease resistance; Somaclonal variation; Pyricularia grisea.
Resumo: | The gene Pi-ar confers resistance to Pyricularia grisea race IB-45 in a somaclone derived from immature panicles of the susceptible rice (Oryza sativa) cultivar Araguaia. RAPD technique was used to identify molecular markers linked to this gene utilizing bulked segregant analysis. Initially, the two parental DNAs from the resistant donor SC09 and 'Araguaia' were analyzed using random primers. Of the 240 primers tested, 203 produced amplification products. The two parental DNAs along with the resistant and susceptible bulks of F2 population were screened using 48 primers that differentiated resistant and susceptible parents. Even though eight primers differentiated the resistant bulk from the susceptible bulk, as well as somaclone SC09 and 'Araguaia', only one primer, OPC02 ('GTGAGGCGTC'), was found to be tightly linked (1.7cM) to the resistance gene of somaclone SC09.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000200010
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582002000200010
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.27 n.2 2002
Fonte: | info:eu-repo/semantics/openAccess