Data/hora: 09/06/2024 04:17:42

Provedor de dados: | Tropical Plant Pathology: fitopatologia brasileira
País: | Brazil
Título: | Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
Autores: | Almeida,Álvaro M. R.; Sakai,Junichi; Hanada,Kaoru; Oliveira,Thalita G.; Belintani,Priscila; Kitajima,Elliot W.; Souto,Eliezer R.; Novaes,Tanara G. de; Nora,Paulo S.
Data: | 2005-08-01
Ano: | 2005
Palavras-chave: | Nucleotide sequence; Elisa; Host range.
Resumo: | A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582005000400005
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.30 n.4 2005
Fonte: | info:eu-repo/semantics/openAccess