Data/hora: 06/06/2024 09:44:08

Provedor de dados: | 102
País: | Brazil
Título: | Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus
Autores: | Souza Júnior,Manoel T.; Nickel,Osmar; Gonsalves,Dennis.
Data: | 2005-08-01
Ano: | 2005
Palavras-chave: | Carica papaya; Potyvirus; Resistance; Breeding; Virus.
Resumo: | Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400004
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582005000400004
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.30 n.4 2005
Fonte: | info:eu-repo/semantics/openAccess