Data/hora: 06/06/2024 01:22:11

Provedor de dados: | Tropical Plant Pathology: fitopatologia brasileira
País: | Brazil
Título: | Coat protein and RNAs of Cole latent virus are not present within chloroplasts of Chenopodium quinoa-infected cells
Autores: | Belintani,Priscila; Gaspar,José O.
Data: | 2003-01-01
Ano: | 2003
Palavras-chave: | Brassica; Electron microscopy; Carlavirus; Virus replication; Plant virus.
Resumo: | Cole latent virus (CoLV), genus Carlavirus, was studied by electron microscopy and biochemical approaches with respect both to the ultrastructure of the Chenopodium quinoa infected cells and to its association with chloroplasts. The CoLV was observed to be present as scattered particles interspersed with membranous vesicles and ribosomes or as dense masses of virus particles. These virus particles reacted by immunolabelling with a polyclonal antibody to CoLV. Morphologically, chloroplasts, mitochondria and nuclei appeared to be unaltered by virus infection and virus particles were not detected in these organelles. However, virus particle aggregates were frequently associated with the outer membrane of chloroplasts and occasionally with peroxisomes. Chloroplasts were purified by Percoll gradient, and the coat protein and virus-associated RNAs were extracted and analyzed by Western and Northern blots respectively. Coat protein and CoLV-associated RNAs were not detected within this organelle. The results presented in this work indicate that the association CoLV/chloroplasts, observed in the ultrastructural studies, might be a casual event in the host cell, and that the virus does not replicate inside the organelle.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000100012
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582003000100012
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.28 n.1 2003
Fonte: | info:eu-repo/semantics/openAccess