Data/hora: 06/06/2024 05:29:50

Provedor de dados: 	103	
País: 	Brazil	
Título: 	Genotoxic effects of the diesel water-soluble fraction on the seahorse Hippocampus reidi (Teleostei: Syngnathidae) during acute exposure	
Autores: 	Santos,Celina Alcoforado; Novaes,Larissa Simões; Gomes,Levy Carvalho.	
Data: 	2010-01-01	
Ano: 	2010	
Palavras-chave: 	Comet assay; Diesel; Marine fish; Micronuclei; Toxicity.	
Resumo: 	The high toxicity of diesel components makes oil spills a threat to the biota in coastal marine environments. The genotoxic effect of the diesel water-soluble fraction (DWSF) on Hippocampus reidi (Ginsburg, 1933) was assessed. Fish were exposed to three different DWSF dilutions for up to 96 hours, and genotoxicity was analyzed using the micronuclei test and the comet assay. The micronuclei test revealed no significant differences between any of the DWSF dilutions and the control in the 24-hours period; however, micronuclei increased in fish exposed to 1:500 and 1:100 DWSF dilutions for 96 hours. For all dilutions, there was an increase in micronuclei in fish exposed for 96 hours when compared to those exposed for 24 hours. The tested dilutions increased frequencies of cell classes 2 (medium damage) and 3 (large damage) in the comet assay after 24 and 96 hours. Fish exposed to DWSF 1:100 exhibited a higher frequency of class 4 (apoptosis) cells in the 96-hours period. All dilutions increased the comet score when compared to the control at 24 and 96 hours. The micronuclei and comet tests were efficient in detecting DWSF genotoxic effects in H. reidi.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600017	
Editor: 	Sociedade Brasileira de Zoologia	
Relação: 	10.1590/S1984-46702010000600017	
Formato: 	text/html	
Fonte: 	Zoologia (Curitiba)  v.27 n.6 2010	
Fonte: 	info:eu-repo/semantics/openAccess