Data/hora: 29/05/2024 06:30:18

Provedor de dados: | Zoologia
País: | Brazil
Título: | Evaluation of lugol solution used for counting rumen ciliates
Autores: | D'Agosto,Marta; Carneiro,Maria Elisa.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Rumen ciliate; Quantitative evaluation; Lugol solution; Technique.
Resumo: | The present study proposes a modification of the technique described by PURSER &amp;amp; MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000300011
Editor: | Sociedade Brasileira de Zoologia
Relação: | 10.1590/S0101-81751999000300011
Formato: | text/html
Fonte: | Revista Brasileira de Zoologia  v.16 n.3 1999
Fonte: | info:eu-repo/semantics/openAccess