Data/hora: 09/06/2024 13:54:17

Provedor de dados: | 114
País: | Argentina
Título: | Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry
Autores: | Asinari,F; Cafrune,E.E; Guzmán,F.A; Conci,L.R; Conci,V.C.
Data: | 2016-06-01
Ano: | 2016
Palavras-chave: | Fragaria x ananassa; Molecular techniques; Screening viruses; Virus diagnosis.
Resumo: | The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3&amp;#8217;UTR region of the viral genome. The cloned cDNA segment was labeled and used as a probe. Six RNA extraction protocols were evaluated, and the modified cetyltrimethylammonium bromide (CTAB) method showed the highest sensitivity level. Leaves at different phenological stages and petioles were evaluated; the highest reaction was observed in old leaves and in petioles.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2016000100004
Editor: | Facultad de Ciencias Agropecuarias. Universidad Nacional de Córdoba
Formato: | text/html
Fonte: | Agriscientia  v.33 n.1 2016
Fonte: | info:eu-repo/semantics/openAccess