Data/hora: 09/06/2024 05:48:39

Provedor de dados: | 117
País: | Chile
Título: | Encapsulated Somatic Embryos and Zygotic Embryos for Obtaining Artificial Seeds of Rauli-Beech (Nothofagus alpina (Poepp. &amp;amp; Endl.) Oerst.)
Autores: | Cartes R,Priscila; Castellanos B,Hermes; Ríos L,Darcy; Sáez C,Katia; Spierccolli H,Scarlette; Sánchez O,Manuel.
Data: | 2009-03-01
Ano: | 2009
Palavras-chave: | Rauli-beech; Somatic embryogenesis; Synthetic seed; Sodium alginate.
Resumo: | Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. &amp;amp; Endl.) Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and 2%, as did the immersion time in calcium chloride (CaCl2), which acts as complexing agent. The artificial endosperm components of the Murashige and Skoog medium (MS) were added, supplemented with 0.5 mg L-1 indolacetic acid (IAA), 0.5 mg L-1 naphthaleneacetic acid (NAA), 2 mg L-1 6-benzylaminopurine (BAP) and 30 g L-1 sucrose. The germinative behaviors of encapsulated somatic and zygotic embryos were evaluated after 4 wk. Comparing the percentages of germination reached by encapsulated somatic and zygotic embryos it was observed that they had similar germinative behavior according to the type of encapsulation applied. However, zygotic embryos substantially exceeded the germination levels reached by somatic embryos, 100% vs. 45% respectively.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100014
Editor: | Instituto de Investigaciones Agropecuarias, INIA
Formato: | text/html
Fonte: | Chilean journal of agricultural research  v.69 n.1 2009