Data/hora: 13/06/2024 02:54:52

Provedor de dados: | Revista Ciência Agronômica (RCA)
País: | Brazil
Título: | Physiological quality evaluation of Piptadenia stipulacea (Benth.) Ducke seeds by tetrazolium test
Autores: | Pereira,Kleane Targino Oliveira; Paiva,Emanoela Pereira de; Souza Neta,Maria Lilia de; Benedito,Clarisse Pereira; Torres,Salvador Barros.
Data: | 2020-01-01
Ano: | 2020
Palavras-chave: | Fabaceae; Biochemical test; Viability; Forestry species; Triphenyl formazan.
Resumo: | ABSTRACT The tetrazolium test is one of the promising alternatives to determine the viability of dormant, recalcitrant and slow-germinating seeds. The objective of this study was to adapt the tetrazolium test methodology to estimate the viability and vigor of Piptadenia stipulacea (Benth.) Ducke seeds. For this, a sample of seeds was classified into three sublots using round sieves, corresponding to six, five and four millimeters in diameter, respectively. Moisture content, imbibition curve, germination, emergence, germination and emergence speed indices, mean germination time and seedling emergence were initially evaluated. The second stage was conducted using a completely randomized experimental design, in 3 x 9 factorial scheme, corresponding to three sublots and nine combinations between concentrations and time of immersion in tetrazolium salt (0.05%/2 h; 0.05%/4 h; 0.05%/6 h; 0.075%/2 h; 0.075%/4 h; 0.075%/6 h; 0.1%/2 h; 0.1%/4 h; 0.1%/6 h), totaling twenty-seven treatments with four replicates of 25 seeds, evaluated separately at 35 and 40 °C. For each treatment, the seeds were divided into four classes: viable and vigorous seeds (class I), viable and non-vigorous (class II), unviable (class III) and dead (class IV). The period of 8 h of pre-imbibition and 4 h of staining in tetrazolium solution at 0.075%, under 35 or 40 °C, is adequate to estimate the viability and vigor of P. stipulacea seeds.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000100419
Editor: | Universidade Federal do Ceará
Relação: | 10.5935/1806-6690.20200019
Formato: | text/html
Fonte: | Revista Ciência Agronômica  v.51 n.1 2020
Fonte: | info:eu-repo/semantics/openAccess