Data/hora: 11/06/2024 09:58:29

Provedor de dados: | Revista Ciência Agronômica (RCA)
País: | Brazil
Título: | Fusarium oxysporum and F. verticillioides associated with damping-off in Pinus spp.
Autores: | Maciel,Caciara Gonzatto; Walker,Clair; Santos,Ricardo Feliciano dos; Muniz,Marlove Fátima Brião; Brum,Daniele Lemos.
Data: | 2017-03-01
Ano: | 2017
Palavras-chave: | ITS; Elongation factor (tef1); Pinus taeda; Pinus elliottii.
Resumo: | ABSTRACT Occurrence of Fusarium spp. is one of the problems, most limiting to growth of seedlings, in nurseries. This pathogen can be transmitted via seeds and causes damages to the seedlings during pre- and post-emergence stages. The present study aimed to identify Fusarium spp. at the species level based on morphological and molecular characteristics and to verify the pathogenicity of these isolates in seeds lots of Pinus elliottii and P. taeda. For this, we used two Fusarium isolates and five lots of Pinus spp. seeds. Morphological characterization was performed based on a key, specific to Fusarium spp. identification, whereas, molecular identification was carried out by amplification and sequencing of the regions from internal transcribed spacer (ITS) and the elongation factor 1-α (tef1). The pathogenicity test was conducted through the contact of the seeds with fungal culture for 48 h, followed by sowing them in sand. The variables evaluated were emergency speed index, percentage of emergency, non-emergency seeds, symptomatic seedlings, and seedling damping-off. One isolate, F1UFSM, was identified as F. verticillioides and another isolate, F2UFSM, was identified as F. oxysporum. Both the isolates were pathogenic to the seeds of Pinus spp., causing a reduction in the percentage of emergence and seedling damping-off.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100134
Editor: | Universidade Federal do Ceará
Relação: | 10.5935/1806-6690.20170015
Formato: | text/html
Fonte: | Revista Ciência Agronômica  v.48 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess