Data/hora: 04/06/2024 08:35:01

Provedor de dados: | 17
País: | United Kingdom
Título: | Accurate estimation of homologue-specific DNA concentration-ratios in cancer samples allows long-range haplotyping
Autores: | Scott L. Carter; Matthew Meyerson; Gad Getz.
Data: | 2011-10-05
Ano: | 2011
Palavras-chave: | Cancer; Genetics &amp; Genomics; Bioinformatics.
Resumo: | Interpretation of allelic copy measurements at polymorphic markers in cancer samples presents distinctive challenges and opportunities. Due to frequent gross chromosomal alterations occurring in cancer (aneuploidy), many genomic regions are present at homologous-allele imbalance. Within such regions, the unequal contribution of alleles at heterozygous markers allows for direct phasing of the haplotype derived from each individual parent. In addition, genome-wide estimates of homologue specific copy- ratios (HSCRs) are important for interpretation of the cancer genome in terms of fixed integral copy-numbers. We describe HAPSEG, a probabilistic method to interpret bi- allelic marker data in cancer samples. HAPSEG operates by partitioning the genome into segments of distinct copy number and modeling the four distinct genotypes in each segment. We describe general methods for fitting these models to data which are suit- able for both SNP microarrays and massively parallel sequencing data. In addition, we demonstrate a specially tailored error-model for interpretation of systematic variations arising in microarray platforms. The ability to directly determine haplotypes from cancer samples represents an opportunity to expand reference panels of phased chromosomes, which may have general interest in various population genetic applications. In addition, this property may be exploited to interrogate the relationship between germline risk and cancer phenotype with greater sensitivity than is possible using unphased genotype. Finally, we exploit the statistical dependency of phased genotypes to enable the fitting of more elaborate sample-level error-model parameters, allowing more accurate estimation of HSCRs in cancer samples.
Tipo: | Manuscript

Identificador:

http://precedings.nature.com/documents/6494/version/1

oai:nature.com:10101/npre.2011.6494.1

http://hdl.handle.net/10101/npre.2011.6494.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License