Data/hora: 11/06/2024 06:42:24

Provedor de dados: 	Nature Precedings	
País: 	United Kingdom	
Título: 	Angiogenesis and Vasculogenesis at 7-Day of Reperfused Acute Myocardial Infarction	
Autores: 	Sheng  Kang; Yue-jin Yang; Qing-zhi Wang; Yue L. I. Li; Yi Tian; Yu-tong Cheng; Wei-feng Shen.	
Data: 	2008-07-10	
Ano: 	2008	
Palavras-chave: 	Molecular Cell Biology; Bioinformatics.	
Resumo: 	Objectives &amp;#xd;&amp;#xa;This study is to investigate the angiogenesis and vasculogenesis at the first week of reperfused acute myocardial infarction (AMI).&amp;#xd;&amp;#xa;Methods &amp;#xd;&amp;#xa;16 of mini-swines (20 to 30 Kg) were randomly assigned to the sham-operated group and the AMI group. The acute myocardial infarction and reperfusion model was created and the pig tail catheter was performed to monitor hemodynamics before left anterior descending coronary artery (LAD) occlusion, 90 min of LAD occlusion and 120 min of LAD reperfusion. Pathologic myocardial tissue was collected at 7-day of LAD reperfusion and further assessed by immunochemistry, dual immunochemistry, in-situ hybridization, real-time quantitative polymerase chain reaction and western blot. &amp;#xd;&amp;#xa;Results &amp;#xd;&amp;#xa;The infarcted area had higher FLK1 mRNA expression than sham-operated area and the normal area (all P&amp;#x3c;0.05), and the infarcted and marginal areas showed higher CD146 protein expression than the sham-operated area (all P&amp;#x3c;0.05), but the microvessel density (CD31 positive expression of microvessels/HP) was not significantly different between the infarcted area and the sham-operated area (8.92&amp;#xb1;3.05 vs 6.43&amp;#xb1;1.54) at 7-day of reperfused acute myocardial infarction (P&amp;#x3e;0.05). &amp;#xd;&amp;#xa;Conclusions &amp;#xd;&amp;#xa;FLK1 and CD146 expression significantly increase in the infarcted and marginal areas, and the microvessel density is not significantly different between the infarcted area and the sham-operated area, suggesting that angiogenesis and vasculogenesis in the infarcted area appear to high frequency of increase in 7-day of reperfused myocardial infarction. &amp;#xd;&amp;#xa;	
Tipo: 	Manuscript	
Identificador: 	http://precedings.nature.com/documents/2066/version/1

oai:nature.com:10101/npre.2008.2066.1

http://hdl.handle.net/10101/npre.2008.2066.1	
Fonte: 	Nature Precedings	
Fonte: 	Creative Commons Attribution 3.0 License