Data/hora: 13/06/2024 23:43:48

Provedor de dados: | 17
País: | United Kingdom
Título: | Homeoprotein Hbx4 represses adhesion molecule governing cytokinesis and development
Autores: | Ji-Sun Kim; Ji-Hui Seo; Hyung-Soon Yim; Sa-Ouk Kang.
Data: | 2010-02-02
Ano: | 2010
Palavras-chave: | Developmental Biology; Molecular Cell Biology.
Resumo: | Homeobox genes encode proteins with a highly conserved DNA-binding motif and provoke morphological diversification of body segments by differentially controlling the expression of downstream targets. Here, we have identified _hbx4_, one of many homeobox genes in _Dictyostelium discoideum_ and investigated its role during growth and development. In suspension, Hbx4-overexpressing cells, Hbx4^OE^, showed defects in cytokinesis and growth rate. During development, Hbx4^OE^ and _hbx4_-disrupting cells, _hbx4&amp;#xaf;_ made differences in shape of mound and slug, cell-type proportioning from wild type KAx3 cells. These phenotypes were similar to those of mutant defective in _cadA_ encoding Ca^2+^-dependent cell adhesion molecule so that we investigated the relationship between _hbx4_ and _cadA_. Overexpression of Hbx4 inhibited the expression of _cadA_ and cAMP also failed to stimulate _cadA_ in Hbx4^OE^. Furthermore, gel mobility shift assay showed the promoter of _cadA_ contained Hbx4-binding site, indicating Hbx4 negatively regulates the expression of _cadA_. Proteome analysis revealed that overexpression of Hbx4 repressed the _rdiA_ and _abpB_ encoding rho guanine nucleotide dissociation inhibitor1, RhoGDI1 and actin bundling protein 34, ABP34, respectively. And the overexpression of _cadA_ in Hbx4^OE^ cells rescued the defects and increased mRNA level of _rdiA_, _abpB_ and one of Rho GTPase, _rac1b_. These results suggested that Hbx4 can modulate cytokinesis, cell sorting and cell-type proportioning by repressing _cadA_ that regulates GTPase-dependent signaling pathway.
Tipo: | Manuscript

Identificador:

http://precedings.nature.com/documents/4194/version/1

oai:nature.com:10101/npre.2010.4194.1

http://hdl.handle.net/10101/npre.2010.4194.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License