Data/hora: 12/06/2024 14:16:52

Provedor de dados: | Nature Precedings
País: | United Kingdom
Título: | Tumor necrosis factor and caspase in response to soluble or microparticle-incorporated drugs in Mycobacterium tuberculosis infection
Autores: | Awadh Bihari Yadav; Amit Misra.
Data: | 2007-09-28
Ano: | 2007
Palavras-chave: | Immunology; Pharmacology.
Resumo: | We compared the effects of microparticles (MP) containig anti-tubercular drugs and those of the drugs themselves on the host macrophage (M&amp;#x3a6;) response to infection with Mycobacterium tuberculosis H37Ra (Mtb). &amp;#xd;&amp;#xa;Mice infected intravenously with M. tb. were either administered rifampicin and isoniazid by oral gavage or through inhalation of biodegradable MP containing the two anti-tubercular drugs. Bronchoalveolar lavage (BAL) was perfomed to recover lung M&amp;#x3a6;, which were cultured and the supernatant analysed for TNF&amp;#x3b1; by ELISA. The murine M&amp;#x3a6; cell line J774 or the human monocyte line THP-1 differentiated with phorbol myristate were infected in vitro and treated with MP or soluble drugs. The kinetics of secretion of TNF&amp;#x3b1; were determined. Caspase-3 activity after infection and treatment was assesed using a substrate cleavage detection kit.&amp;#xd;&amp;#xa;MP, but not soluble drugs, strongly induced TNF&amp;#x3b1; in infected cells. Uninfected cells also responded to MP, although less strongly. Caspase-3 was observed to be upregulated&amp;#xd;&amp;#xa;We conclude that MP treatment induces infected M&amp;#x3a6; to upregulate the Th1 cytokine TNF&amp;#x3b1; and Caspase-3, creating conditions for induction of apoptosis in these cells as a strategy to overcome infection.&amp;#xd;&amp;#xa;
Tipo: | Poster

Identificador:

http://precedings.nature.com/documents/1190/version/1

oai:nature.com:10.1038/npre.2007.1190.1

http://dx.doi.org/10.1038/npre.2007.1190.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License