Data/hora: 03/06/2024 04:42:08

Provedor de dados: | 17
País: | United Kingdom
Título: | 3D model of lamprey estrogen receptor with estradiol and 15[alpha]-hydroxy-estradiol
Autores: | Michael E. Baker; David J. Chang; Charlie Chandsawangbhuwana.
Data: | 2009-01-09
Ano: | 2009
Palavras-chave: | Developmental Biology; Ecology; Earth &amp; Environment.
Resumo: | Lamprey, a basal vertebrate, contains orthologs of the estrogen receptor (ER), progesterone receptor and corticoid receptor.  A perplexing property of lamprey is that 15[alpha]-hydroxy-steroids are active steroids.  For example, 15[alpha]-hydroxy-estradiol [15[alpha]-OH-E2] is the estrogen, instead of estradiol (E2).  To investigate how 15[alpha]-OH-E2 binds lamprey ER, we constructed a 3D model of the lamprey ER with E2 and 15[alpha]-OH-E2.  Our 3D model shows that S[delta] on Met-409 can form a hydrogen bond with the 15[alpha]-hydroxyl on 15[alpha]-OH-E2.  In human ER[alpha], the corresponding residue Ile-424 has a van der Waals contact with 15[alpha]-OH-E2.  BLAST analysis of GenBank indicates that among vertebrate ERs, only lamprey ER contains a methionine at this position.  Thus, the contact between S[delta] on Met-409 and 15[alpha]-OH-E2 is unique.  Our 3D model of lamprey ER should prove useful in virtual screening of chemical libraries to identify compounds for controlling reproduction in sea lamprey, an environmental pest in the Great Lakes in the USA.
Tipo: | Manuscript

Identificador:

http://precedings.nature.com/documents/2768/version/1

oai:nature.com:10101/npre.2009.2768.1

http://hdl.handle.net/10101/npre.2009.2768.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License