Data/hora: 02/06/2024 07:29:09

Provedor de dados: | 19
País: | Japan
Título: | Exposure to 50-Hz electric fields on stress response initiated by infection with the protozoan parasite, Toxoplasma gondii, in mice
Autores: | Hori, Takuya; Harakawa, Shinji; Inoue, Noboru; Nagasawa, Hideyuki; Kariya, Tatsuya.
Data: | 2006
Ano: | 2006
Palavras-chave: | 50-Hz; Electric field; Hsp-60; Stress response; Survival rate.
Resumo: | The present study was aimed to examine whether a 50-Hz electric field (EF) affects biological stress responses. Male C57BL/6J mice were exposed to either 50-Hz EF (45 kV/m) or given a sham exposure for 30 minutes per day for 1, 2 or 4 weeks and then either mock inoculated or inoculated with the protozoan parasite, Toxoplasma gondii (T. gondii) PLK strain. Survival rate was calculated in mice infected with T. gondii after exposure to EF and the serum antibody levels to heat shock protein 60 (Hsp-60), and mRNA levels for Hsp-60, IFN-γ and IL-12p35 in mouse peripheral leucocytes were determined. As a result, no statistically significant difference was found in survival rate between mice exposed to EF and those in the sham exposure group. When mice were inoculated with T. gondii, plasma level of anti-Hsp-60 antibody increased gradually until day 16 after inoculation and remained at an elevated value until the end of the experimental period. On day 2 and 9 after inoculation, the increment in anti-Hsp-60 antibody level was significantly suppressed in mice exposed to EF as compared to those in the sham exposure group (p&lt;0.05). Differences in changes in mRNA levels in Hsp-60, IFN- γ and IL-12p35 were not statistically significant. Taken together, our findings indicate that exposure to 50-Hz EF does not affect viability of mice infected with T. gondii but seems to slightly play inhibitory on the early phase of stress responses.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/997
Editor: | National Research Center for Protozoan Diseases
Formato: | application/pdf