Data/hora: 10/06/2024 07:33:04

Provedor de dados: | Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
País: | Japan
Título: | Effect of Zona Incision by Piezo-Micromanipulator (ZIP) on In Vitro Fertilization in 21 Transgenic Mice Lines
Autores: | KAWASE, Yosuke; TACHIBE, Takanori; HANI, Toshio; TATEISHI, Hiromi; JISHAGE, Kou-ichi; SUZUKI, Hiroshi; 鈴木, 宏志.
Data: | 2009
Ano: | 2009
Palavras-chave: | Frozen-thawed spermatozoa; IVF; ZIP.
Resumo: | Zona incision using a piezo-micromanipulator (ZIP) has been demonstrated to be effective for in vitro fertilization (IVF) using cryopreserved C57BL/6 spermatozoa. In this study, ZIP oocytes inseminated with frozen-thawed genetically modified C57BL/6J or FVB mice spermatozoa (21 lines) showed fertilization rates of 22-75% and live fetus rates of 8-49%. In 6 of the lines, the fertilization rates for oocytes compared with ZIP (42-75%) were significantly higher than that of nontreated oocytes (0-50%). Using only 90 oocytes for IVF with ZIP, 5 breeding pairs were produced from cryopreserved genetically modified mice spermatozoa. Our results indicate that application of the ZIP technique is effective for IVF using cryopreserved genetically modified mouse spermatozoa.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/3017
Editor: | 日本実験動物学会
Formato: | application/pdf
Fonte: | 日本実験動物学会