Data/hora: 13/06/2024 01:30:58

Provedor de dados: | Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
País: | Japan
Título: | Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential
Autores: | Dautu, George; Munyaka, Biscah; Carmen, Gabriella; Zhang, Guohong; Omata, Yoshitaka; Xuan, Xuenan; Igarashi, Makoto.
Data: | 2007-07
Ano: | 2007
Palavras-chave: | Toxoplasma gondii; DNA; Deoxyribonucleic acid; DNA immunization; MIC2; Microneme protein-2; M2AP; MIC2 associated protein; AMA1; Apical membrane antigen-1; BAG1; Bradyzoite antigen-1; IgG; Immunoglobulin G.
Resumo: | A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-γ synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/1031
Editor: | Elsevier
Formato: | application/pdf