Data/hora: 13/06/2024 04:05:49

Provedor de dados: | Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
País: | Japan
Título: | Transcriptional analyses of Trypanosoma brucei gambiense from infected mice and in vitro culture
Autores: | Kuboki, Noritaka; Kibe, Michael K; Thekisoe, Oriel M. M.; Sugimoto, Chihiro; Inoue, Noboru.
Data: | 2007-06
Ano: | 2007
Palavras-chave: | Differential expression; Infection; Trypanosoma brucei.
Resumo: | With the hypothesis that African trypanosomes could have in vivo specific genes for adaptation to host’s environment, the present study was conducted by using suppressive subtractive hybridization (SSH) technique to seek the highly expressed genes especially in host. A total of 328 clones from the in vivo SSH library and that of 160 clones from the in vitro SSH library were analyzed in order to determine their expression levels, but none of the above-mentioned genes showed differential expression. This indicates that no trypanosome genes could be differentially expressed either the in vivo or in vitro propagated trypanosomes. Alternatively, there might be limitation for detecting specifically expressed genes in African trypanosomes using this method, because of their polycistronic gene expression.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/1052
Editor: | National Research Center for Protozoan Diseases
Formato: | application/pdf
Fonte: | National Research Center for Protozoan Diseases