Data/hora: 10/06/2024 17:06:27

Provedor de dados: 	19	
País: 	Japan	
Título: 	Determination of Prostaglandins in the Colostrum of Dairy Cattle by Thermospray High-Performance Liquid Chromatography-Mass Spectrometry.	
Autores: 	FUKUSHIMA, Michihiro; NAKANO, Masuo; ENDO, Tsuyoshi; NEGISHI, Takashi; 福島, 道広.	
Data: 	1993	
Ano: 	1993	
Palavras-chave: 	Thermospray liquid chromatography-mass spectrometry; Prostaglandin; Colostrum; Dairy cattle.	
Resumo: 	A thermospray high-performance liquid chromatography-mass spectrometry (TSP HPLC-MS) method is described for the analysis of prostaglandins (PGs) E1 and E2, which have several physiological actions at the trace level. HPLC separation was carried out on a reversed-phase column by using 50mM ammonium acetate (pH 4.5)-acetonitrile (7:3, v/v) as the mobile phase at a flow rate of 1.0ml/min. In MS detection, the vaporizer temperature was set at 220°C and the ion-source block temperature at 264°C. The mass spectra obtained from PGs typically exhibited the [M+NH4]+ ion (5 to 30% relative intensity) and several fragment ions due to sequential losses of water from both the [M+H]+ and [M+NH4]+ ions. PGE1 (46.9ppb) and PGE2 (1.9ppb) in the colostrum of dairy cattle were identified and quantitated. The PGE1 concentration in the colostrum and mature milk of dairy cattle showed a similar pattern to that in humans.	
Idioma: 	Inglês	
Identificador: 	http://ir.obihiro.ac.jp/dspace/handle/10322/4042	
Editor: 	日本分析化学会	
Fonte: 	日本分析化学会