Data/hora: 16/06/2024 09:31:34

Provedor de dados: | Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
País: | Japan
Título: | In vitro and in vivo effects of the phytohormone inhibitor fluridone against Neospora caninum infection
Autores: | Ybañez, Rochelle Haidee D.; Leesombun, Arpron; Nishimura, Maki; Matsubara, Ryuma; Kojima, Mikiko; Sakakibara, Hitoshi; Nagamune, Kisaburo; Nishikawa, Yoshifumi.
Data: | 2016-08
Ano: | 2016
Palavras-chave: | Neospora caninum; Fluridone; Abscisic acid.
Resumo: | Neospora caninum causes abortion and stillbirth in cattle. Identification of effective drugs against this parasite remains a challenge. Previous studies have suggested that disruption of abscisic acid (ABA)-mediated signaling in apicomplexan parasites such as Toxoplasma gondii offers a new drug target. In this study, the ABA inhibitor, fluridone (FLU), was evaluated for its action against N. caninum. Production of endogenous ABA within N. caninum was confirmed by ultra-performance liquid chromatography-tandem quadruple mass spectrometry. Subsequently, FLU treatment efficacy was assessed using in vitro. Results revealed that FLU inhibited the growth of N. caninum and T. gondii in vitro (IC50 143.1 +/- 43.96 mu M and 330.6 +/- 5238 mu M, respectively). However, FLU did not affect parasite replication at 24 h post-infection, but inhibited egress of N. caninum thereafter. To evaluate the effect of FLU in vivo, N. caninum-infected mice were treated with FLU for 15 days. FLU treatment appeared to ameliorate acute neosporosis induced by lethal parasite challenge. Together, our data shows that ABA might control egress in N. caninum. Therefore, FLU has potential as a candidate drug for the treatment of acute neosporosis.
Idioma: | Inglês

Identificador:

http://ir.obihiro.ac.jp/dspace/handle/10322/4491

info:doi/10.1016/j.parint.2016.03.009

Editor: | Elsevier
Fonte: | This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. &lt;http://creativecommons.org/lisenses/by-nc-nd/4.0/&gt;